Assessment of toxicity changes induced by exposure of human cells to lunar dust simulant.
AHH-1 cell
BEAS-2B cell
Lunar dust simulant
RNA-seq
Toxicity of lunar dust
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
21 10 2024
21 10 2024
Historique:
received:
07
03
2024
accepted:
02
08
2024
medline:
22
10
2024
pubmed:
22
10
2024
entrez:
21
10
2024
Statut:
epublish
Résumé
The toxicity of lunar dust (LD) to astronauts' health has been confirmed in the Apollo missions and subsequent biological experiments. Therefore, it is crucial to understand the biological toxicity of lunar dust for future human missions to the Moon. In this study, we exposed human lung epithelial cells (BEAS-2B) and peripheral blood B lymphocytes (AHH-1) to varying concentrations (0, 500, 1000, and 1500 μg/ml) of a lunar dust simulant (LDS) called CLDS-i for 24 and 48 h. The results provided the following key findings: (1) LDS induction of cell damage occurred through oxidative stress, with the levels of reactive oxygen species (ROS) in BEAS-2B cells being dependent on the duration of exposure. (2) Necrosis and early apoptosis were observed in BEAS-2B cells and AHH-1 cells, respectively. In addition, both cells showed lysosomal damage. (3) Genes CXCL1, SPP1, CSF2, MMP1, and POSTN are implicated in immune response and cytoskeletal arrangement regulation in BEAS-2B cells. Considering the similarities in composition and properties between CLDS-i and real lunar dust, our findings not only enhance the understanding of LDS toxicity, but also contribute to a better comprehension of the genomic alterations and molecular mechanisms underlying cellular toxicity induced by LD. These insights will contribute to the development of a biotoxicology framework aimed at safeguarding the health of astronauts and, consequently, facilitating future human missions to the Moon.
Identifiants
pubmed: 39433758
doi: 10.1038/s41598-024-69259-w
pii: 10.1038/s41598-024-69259-w
doi:
Substances chimiques
Reactive Oxygen Species
0
Cosmic Dust
0
Dust
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
24781Subventions
Organisme : the National Natural Science Foundation of China
ID : Grant No.12172146
Informations de copyright
© 2024. The Author(s).
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