Interaction of SARS-CoV-2 Spike protein with ACE2 induces cortical actin modulation, including dephosphorylation of ERM proteins and reduction of cortical stiffness.
ACE2
Cell stiffness
Cortical actin
ERM
SARS-CoV-2
Journal
Human cell
ISSN: 1749-0774
Titre abrégé: Hum Cell
Pays: Japan
ID NLM: 8912329
Informations de publication
Date de publication:
22 Oct 2024
22 Oct 2024
Historique:
received:
29
05
2024
accepted:
30
08
2024
medline:
22
10
2024
pubmed:
22
10
2024
entrez:
22
10
2024
Statut:
epublish
Résumé
Cell surface cortical actin is a regulatory target for viral infection. We aimed to investigate the effect of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on host cell cortical stiffness, an indicator of cortical actin structure. The receptor-binding domain (RBD) of SARS-CoV-2 Spike (S) protein induced a reduction in cortical stiffness in ACE2-expressing cells. The interaction of RBD with ACE2 caused the inactivation of Ezrin/Radixin/Moesin (ERM) proteins. We further investigated the effects of the RBD of SARS-CoV-2 Omicron variants, BA.1 and BA.5. These RBDs influenced cortical stiffness depending on their affinity for ACE2. Our study provides the first evidence that the interaction of the SARS-CoV-2 S protein with ACE2 induces mechanobiological signals and attenuates the cortical actin.
Identifiants
pubmed: 39436480
doi: 10.1007/s13577-024-01142-2
pii: 10.1007/s13577-024-01142-2
doi:
Substances chimiques
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Angiotensin-Converting Enzyme 2
EC 3.4.17.23
Actins
0
Microfilament Proteins
0
Cytoskeletal Proteins
0
ACE2 protein, human
EC 3.4.17.23
moesin
144131-77-1
Membrane Proteins
0
ezrin
0
radixin
144517-21-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3Subventions
Organisme : Japan Society for the Promotion of Science
ID : JP21K04797
Organisme : Japan Society for the Promotion of Science
ID : JP24K15731
Organisme : Ministry of Education, Culture, Sports, Science and Technology
ID : JPMXP0621467946
Organisme : Adaptable and Seamless Technology Transfer Program through Target-Driven R and D
ID : JPMJTR21U4
Organisme : Institute of Environmental Science and Technology, The University of Kitakyushu
ID : Grant for Young Scientists
Informations de copyright
© 2024. The Author(s) under exclusive licence to Japan Human Cell Society.
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