Structuring lipid nanoparticles, DNA, and protein corona into stealth bionanoarchitectures for in vivo gene delivery.

Nanoparticles / chemistry Protein Corona / chemistry DNA / chemistry Animals Humans Gene Transfer Techniques Mice Genetic Therapy / methods COVID-19 / virology Lipids / chemistry SARS-CoV-2 / genetics Transfection / methods Polyethylene Glycols / chemistry Female Particle Size Liposomes

Journal

Nature communications

ISSN: 2041-1723

Titre abrégé: Nat Commun

Pays: England

ID NLM: 101528555

Informations de publication

Date de publication:
23 Oct 2024

Historique:

received: 27 12 2023

accepted: 16 10 2024

medline: 23 10 2024

pubmed: 23 10 2024

entrez: 22 10 2024

Statut: epublish

Résumé

Lipid nanoparticles (LNPs) play a crucial role in addressing genetic disorders, and cancer, and combating pandemics such as COVID-19 and its variants. Yet, the ability of LNPs to effectively encapsulate large-size DNA molecules remains elusive. This is a significant limitation, as the successful delivery of large-size DNA holds immense potential for gene therapy. To address this gap, the present study focuses on the design of PEGylated LNPs, incorporating large-sized DNA, departing from traditional RNA and ionizable lipids. The resultant LNPs demonstrate a unique particle morphology. These particles were further engineered with a DNA coating and plasma proteins. This multicomponent bionanoconstruct exhibits enhanced transfection efficiency and safety in controlled laboratory settings and improved immune system evasion in in vivo tests. These findings provide valuable insights for the design and development of bionanoarchitectures for large-size DNA delivery, opening new avenues for transformative gene therapies.

Identifiants

DOI: 10.1038/s41467-024-53569-8 PMID: 39438484

pubmed: 39438484

doi: 10.1038/s41467-024-53569-8

pii: 10.1038/s41467-024-53569-8

doi:

Substances chimiques

Protein Corona 0

DNA 9007-49-2

Lipid Nanoparticles 0

Lipids 0

Polyethylene Glycols 3WJQ0SDW1A

Liposomes 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

9119

Informations de copyright

Références

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