3D Neurovascular Unit Tissue Model to Assess Responses to Traumatic Brain Injury.
3D human tissue
immune response
neurovascular unit
tissue engineering
traumatic brain injury
Journal
Journal of biomedical materials research. Part A
ISSN: 1552-4965
Titre abrégé: J Biomed Mater Res A
Pays: United States
ID NLM: 101234237
Informations de publication
Date de publication:
23 Oct 2024
23 Oct 2024
Historique:
revised:
26
09
2024
received:
09
07
2024
accepted:
07
10
2024
medline:
23
10
2024
pubmed:
23
10
2024
entrez:
23
10
2024
Statut:
aheadofprint
Résumé
The neurovascular unit (NVU) is a critical interface in the central nervous system that links vascular interactions with glial and neural tissue. Disruption of the NVU has been linked to the onset and progression of neurodegenerative diseases. Despite its significance the NVU remains challenging to study in a physiologically relevant manner. Here, a 3D cell triculture model of the NVU is developed that incorporates human primary brain microvascular endothelial cells, astrocytes, and pericytes into a tissue system that can be sustained in vitro for several weeks. This tissue model helps recapitulate the complexity of the NVU and can be used to interrogate the mechanisms of disease and cell-cell interactions. The NVU tissue model displays elevated cell death and inflammatory responses following mechanical damage, to emulate traumatic brain injury (TBI) under controlled laboratory conditions, including lactate dehydrogenase (LDH) release, elevated inflammatory markers TNF-α and monocyte chemoattractant cytokines MCP-2 and MCP-3 and reduced expression of the tight junction marker ZO-1. This 3D tissue model serves as a tool for deciphering mechanisms of TBIs and immune responses associated with the NVU.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Israel Science Foundation
ID : 3605/21
Organisme : U.S. Department of Defense
Organisme : NIH HHS
ID : 10.13039/100000002
Pays : United States
Organisme : NIH HHS
ID : P41EB027062
Pays : United States
Organisme : NIH HHS
ID : R01AG061838
Pays : United States
Organisme : Department of Defense
ID : W81XWH2211065
Organisme : Department of Defense
ID : W911NF-23-1-0276
Informations de copyright
© 2024 Wiley Periodicals LLC.
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