Effect of particle size on dissolution of different chemical components in Codonopsis pilosula.
Codonopsis pilosula
UHPLC‐Q‐Exactive Orbitrap MS/MS
boiling powder
immunosuppression
molecular docking
Journal
Biomedical chromatography : BMC
ISSN: 1099-0801
Titre abrégé: Biomed Chromatogr
Pays: England
ID NLM: 8610241
Informations de publication
Date de publication:
23 Oct 2024
23 Oct 2024
Historique:
received:
04
09
2024
accepted:
02
10
2024
medline:
23
10
2024
pubmed:
23
10
2024
entrez:
23
10
2024
Statut:
aheadofprint
Résumé
Codonopsis pilosula (Franch.) Nannf. is a traditional herb for treating immunosuppression. C. pilosula boiling powder (CP-BP) contains particles of a small size made from C. pilosula decoction pieces (CP-DP). It is still unclear how changes in particle size during the decoction process affect the dissolution of various chemical components in C. pilosula. Herein, an ultra-high-performance liquid chromatography-quadrupole-Exactive Orbitrap mass spectrometry technique was established to characterize the components of CP-BP and CP-DP decoctions. The contents of the components were evaluated based on the relative peak area, extract yield, and alcohol solubility rate. A total of 71 compounds were finally identified, and their content in the CP-BP decoction was generally higher than that in the CP-DP decoction. Alkaloids had the highest average content, whereas terpenoids were the most affected by changes in particle size. In addition, immunosuppression was used as model to investigate whether these changes have practical significance. The results of network pharmacology suggested that the phosphoinositide 3-kinase (PI3K)-Akt pathway may be a potential pathway of C. pilosula for treating immunosuppression. The results of molecular docking indicated that compounds with large content variations have good docking affinity with key targets (epidermal growth factor receptor [EGFR], prostaglandin-endoperoxide synthase 2 [PTGS2], and peroxisome proliferator-activated receptor gamma [PPARG]). These results provide an important reference for further development and use of C. pilosula.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e6026Subventions
Organisme : Supporting plan for leading talents above provincial level in Yantai
ID : 10073801
Organisme : Shandong Taishan Scholars Young Expert Project
ID : tsqn202103110
Organisme : Shandong Province Youth Talents Introducing and Cultivating Program-Innovative Research
ID : 10073004
Informations de copyright
© 2024 John Wiley & Sons, Ltd.
Références
Bai, R. B., Zhang, Y. J., Fan, J. M., Jia, X. S., Li, D., Wang, Y. P., Zhou, J., Yan, Q., & Hu, F. D. (2020). Immune‐enhancement effects of oligosaccharides from Codonopsis pilosula on cyclophosphamide induced immunosuppression in mice. Food and Function, 11, 3306–3315. https://doi.org/10.1039/c9fo02969a
Chen, J. J., Li, T., Huang, D. H., Gong, W. X., Tian, J. S., Gao, X. X., Qin, X. M., Du, G. H., & Zhou, Y. Z. (2023). Integrating UHPLC‐MS/MS quantitative analysis and exogenous purine supplementation to elucidate the antidepressant mechanism of Chaigui granules by regulating purine metabolism. Journal of Pharmaceutical Analysis, 13, 1562–1576. https://doi.org/10.1016/j.jpha.2023.08.008
Damoulakis, G., Gambardella, L., Rossman, K. L., Lawson, C. D., Anderson, K. E., Fukui, Y., Welch, H. C., Der, C. J., Stephens, L. R., & Hawkins, P. T. (2014). P‐Rex1 directly activates RhoG to regulate GPCR‐driven Rac signalling and actin polarity in neutrophils. Journal of Cell Science, 127, 2589–2600. https://doi.org/10.1242/jcs.153049
Fan, C. L., Wang, G., Chen, M., Li, Y., Tang, X. Y., & Dai, Y. (2023). Therapeutic potential of alkaloid extract from Codonopsis radix in alleviating hepatic lipid accumulation: Insights into mitochondrial energy metabolism and endoplasmic reticulum stress regulation in NAFLD mice. Chinese Journal of Natural Medicines, 21, 411–422. https://doi.org/10.1016/S1875-5364(23)60403-0
Fruman, D. A., Chiu, H. Y., Hopkins, B. D., Bagrodia, S., Cantley, L. C., & Abraham, R. T. (2017). The PI3K pathway in human disease. Cell, 170, 605–635. https://doi.org/10.1016/j.cell.2017.07.029
Gong, Z., Li, Q., Shi, J. Y., Li, P. S., Hua, L., Shultz, L. D., & Ren, G. W. (2023). Immunosuppressive reprogramming of neutrophils by lung mesenchymal cells promotes breast cancer metastasis. Science Immunology, 8, eadd5204. https://doi.org/10.1126/sciimmunol.add5204
Guerau‐de‐Arellano, M., Piedra‐Quintero, Z. L., & Tsichlis, P. N. (2022). Akt isoforms in the immune system. Frontiers in Immunology, 13, 990874. https://doi.org/10.3389/fimmu.2022.990874
Han, L., Bai, L. L., Qu, C. J., Dai, E. Y., Liu, J., Kang, R., Zhou, D., Tang, D. L., & Zhao, Y. N. (2021). PPARG‐mediated ferroptosis in dendritic cells limits antitumor immunity. Biochemical and Biophysical Research Communications, 576, 33–39. https://doi.org/10.1016/j.bbrc.2021.08.082
He, J. Y., Zhu, S., Goda, Y., Cai, S. Q., & Komatsu, K. (2014). Quality evaluation of medicinally‐used Codonopsis species and Codonopsis radix based on the contents of pyrrolidine alkaloids, phenylpropanoid and polyacetylenes. Journal of Natural Medicines, 68, 326–339. https://doi.org/10.1007/s11418-013-0801-0
Jiang, Y. P., Guo, Q. L., Liu, Y. F., & Shi, J. G. (2016). Codonopiloneolignanin A, a polycyclic neolignan with a new carbon skeleton from the roots of Codonopsis pilosula. Chinese Chemical Letters, 27, 55–58. https://doi.org/10.1016/j.cclet.2015.11.009
Jiang, Y. P., Liu, Y. F., Guo, Q. L., Xu, C. B., Zhu, C. G., & Shi, J. G. (2016). Sesquiterpene glycosides from the roots of Codonopsis pilosula. Acta Pharmaceutica Sinica B, 6, 46–54. https://doi.org/10.1016/j.apsb.2015.09.007
Johann, A. M., von Knethen, A., Lindemann, D., & Brüne, B. (2006). Recognition of apoptotic cells by macrophages activates the peroxisome proliferator‐activated receptor‐gamma and attenuates the oxidative burst. Cell Death and Differentiation, 13, 1533–1540. https://doi.org/10.1038/sj.cdd.4401832
Kim, E. Y., Kim, J. A., Jeon, H. J., Kim, S., Kim, Y. H., Kim, H. Y., & Whang, W. K. (2014). Chemical fingerprinting of Codonopsis pilosula and simultaneous analysis of its major components by HPLC‐UV. Archives of Pharmacal Research, 37, 1148–1158. https://doi.org/10.1007/s12272-014-0335-3
Kulkarni, S., Sitaru, C., Jakus, Z., Anderson, K. E., Damoulakis, G., Davidson, K., Hirose, M., Juss, J., Oxley, D., Chessa, T. A., Ramadani, F., Guillou, H., Segonds‐Pichon, A., Fritsch, A., Jarvis, G. E., Okkenhaug, K., Ludwig, R., Zillikens, D., Mocsai, A., … Hawkins, P. T. (2011). PI3Kβ plays a critical role in neutrophil activation by immune complexes. Science Signaling, 4, ra23. https://doi.org/10.1126/scisignal.2001617
Li, Y. H., Liu, Y. S., Sun, Y., Ma, S. M., Ma, C. M., Zhou, H. P., Chen, G. E., Liu, L., & Cai, D. (2022). Study on the mechanism of Yupingfeng powder in the treatment of immunosuppression based on UPLC•QTOF•MS, network pharmacology and molecular biology verification. Life Sciences, 289, 120211. https://doi.org/10.1016/j.lfs.2021.120211
Liu, G., Wei, X. P., Li, H. J., Gao, S. M., Lin, W. W., Cheng, M., Yao, S. J., & Zhang, B. G. (2018). Studies and reports on edible situation of Codonopsis radix. Modern Chinese Medicine, 20, 892–898. https://doi.org/10.13313/j.issn.1673-4890.20180211002
Marshall, N. A., Galvin, K. C., Corcoran, A. B., Boon, L., Higgs, R., & Mills, K. H. (2012). Immunotherapy with PI3K inhibitor and toll‐like receptor agonist induces IFN‐γ+IL‐17+ polyfunctional T cells that mediate rejection of murine tumors. Cancer Research, 72, 581–591. https://doi.org/10.1158/0008-5472.CAN-11-0307
Mei, Q. X., Zheng, Y. L., Luo, R., Liang, Q., & Jin, S. Y. (2023). The ancient application and modern research progress of traditional Chinese medicine boiled powder. Lishizhen Medicine and Materia Medica Research, 34, 2225–2228. https://doi.org/10.3969/j.issn.1008-0805.2023.09.49
Ming, K., He, M., Su, L. L., Du, H. X., Wang, D. Y., Wu, Y., & Liu, J. G. (2020). The inhibitory effect of phosphorylated Codonopsis pilosula polysaccharide on autophagosomes formation contributes to the inhibition of duck hepatitis A virus replication. Poultry Science, 99, 2146–2156. https://doi.org/10.1016/j.psj.2019.11.060
Qin, F. Y., Cheng, L. Z., Yan, Y. M., Liu, B. H., & Cheng, Y. X. (2018). Two novel proline‐containing catechin glucoside from water‐soluble extract of Codonopsis pilosula. Molecules, 23, 180. https://doi.org/10.3390/molecules23010180
Sabbah, D. A., Hajjo, R., & Sweidan, K. (2020). Review on epidermal growth factor receptor (EGFR) structure, signaling pathways, interactions, and recent updates of EGFR inhibitors. Current Topics in Medicinal Chemistry, 20, 815–834. https://doi.org/10.2174/1568026620666200303123102
Shi, J. C., Gao, X. X., Zhang, A. R., Qin, X. M., & Du, G. H. (2022). Characterization of multiple chemical components of GuiLingJi by UHPLC‐MS and 1H NMR analysis. Journal of Pharmaceutical Analysis, 12, 460–469. https://doi.org/10.1016/j.jpha.2021.09.013
Su, J. S., Qin, F. Y., Liu, Y., & Zhang, Y. (2021). Four new polyynes from Codonopsis pilosula collected in Yunnan province, China. Natural Product Research, 35, 3548–3555. https://doi.org/10.1080/14786419.2020.1712390
Yang, C. X., Gou, Y. Q., Chen, J. Y., An, J., Chen, W. X., & Hu, F. D. (2013). Structural characterization and antitumor activity of a pectic polysaccharide from Codonopsis pilosula. Carbohydrate Polymers, 98, 886–895. https://doi.org/10.1016/j.carbpol.2013.06.079
Yang, W. L., & Frucht, H. (2001). Activation of the PPAR pathway induces apoptosis and COX‐2 inhibition in HT‐29 human colon cancer cells. Carcinogenesis, 22, 1379–1383. https://doi.org/10.1093/carcin/22.9.1379
Zheng, T., Cheng, L. Z., Yan, Y. M., Liu, B. H., Qin, F. Y., Xu, F. R., & Cheng, Y. X. (2018). Two new triterpenoids from the roots of Codonopsis pilosula. Molecules, 23, 383. https://doi.org/10.3390/molecules23020383
Zou, Y. F., Li, C. Y., Fu, Y. P., Feng, X., Peng, X., Feng, B., Li, L. X., Jia, R. Y., Huang, C., Song, X., Lv, C., Ye, G., Zhao, L., Li, Y. P., Zhao, X. H., Yin, L. Z., & Yin, Z. Q. (2022). Restorative effects of inulin from Codonopsis pilosula on intestinal mucosal immunity, anti‐inflammatory activity and gut microbiota of immunosuppressed mice. Frontiers in Pharmacology, 13, 786141. https://doi.org/10.3389/fphar.2022.786141
Zou, Y. F., Zhang, Y. Y., Paulsen, B. S., Rise, F., Chen, Z. L., Jia, R. Y., Li, L. X., Song, X., Feng, B., Tang, H. Q., Huang, C., Ye, G., & Yin, Z. Q. (2021). New pectic polysaccharides from Codonopsis pilosula and Codonopsis tangshen: Structural characterization and cellular antioxidant activities. Journal of the Science of Food and Agriculture, 101, 6043–6052. https://doi.org/10.1002/jsfa.11261
Zou, Y. F., Zhang, Y. Y., Paulsen, B. S., Rise, F., Chen, Z. L., Jia, R. Y., Li, L. X., Song, X., Feng, B., Tang, H. Q., Huang, C., & Yin, Z. Q. (2020). Structural features of pectic polysaccharides from stems of two species of radix Codonopsis and their antioxidant activities. International Journal of Biological Macromolecules, 159, 704–713. https://doi.org/10.1016/j.ijbiomac.2020.05.083