Actin dynamics switches two distinct modes of endosomal fusion in yolk sac visceral endoderm cells.
actin
cell biology
fusion
late endosome
lysosome
mouse
mouse embryo
yolk sac visceral endoderm
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
23 Oct 2024
23 Oct 2024
Historique:
medline:
23
10
2024
pubmed:
23
10
2024
entrez:
23
10
2024
Statut:
epublish
Résumé
Membranes undergo various patterns of deformation during vesicle fusion, but how this membrane deformation is regulated and contributes to fusion remains unknown. In this study, we developed a new method of observing the fusion of individual late endosomes and lysosomes by using mouse yolk sac visceral endoderm cells that have huge endocytic vesicles. We found that there were two distinct fusion modes that were differently regulated. In homotypic fusion, two late endosomes fused quickly, whereas in heterotypic fusion they fused to lysosomes slowly. Mathematical modeling showed that vesicle size is a critical determinant of these fusion types and that membrane fluctuation forces can overcome the vesicle size effects. We found that actin filaments were bound to late endosomes and forces derived from dynamic actin remodeling were necessary for quick fusion during homotypic fusion. Furthermore, cofilin played a role in endocytic fusion by regulating actin turnover. These data suggest that actin promotes vesicle fusion for efficient membrane trafficking in visceral endoderm cells.
Identifiants
pubmed: 39441732
doi: 10.7554/eLife.95999
pii: 95999
doi:
pii:
Substances chimiques
Actins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministry of Education, Culture, Sports, Science and Technology
ID : KAKENHI (17024006)
Organisme : Japan Society for the Promotion of Science
ID : KAKENHI (JP22H04926)
Organisme : Takeda Science Foundation
ID : 2022039209
Informations de copyright
© 2024, Koike et al.
Déclaration de conflit d'intérêts
SK, MT, MT, TO, TN, KK, MM No competing interests declared
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