Development of a colloidal gold immunochromatographic test strip for detecting the smooth Brucella.
Gold Colloid
/ chemistry
Chromatography, Affinity
/ methods
Brucellosis
/ diagnosis
Brucella
/ immunology
Animals
Antibodies, Monoclonal
/ immunology
Sensitivity and Specificity
Humans
Brucella melitensis
/ immunology
Brucella abortus
/ immunology
Reagent Strips
Brucella suis
/ immunology
Lipopolysaccharides
/ analysis
Immunoassay
/ methods
Colloidal gold immunochromatographic assay (GICA)
LPS
Monoclonal antibody
Rapid test
Smooth Brucella
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
23 10 2024
23 10 2024
Historique:
received:
28
05
2024
accepted:
09
10
2024
medline:
24
10
2024
pubmed:
24
10
2024
entrez:
24
10
2024
Statut:
epublish
Résumé
Brucellosis, caused by Gram-negative Brucella, spreads in human and animal populations through contact with infected animals and products. Developing a rapid and sensitive detection technology for pathogen is crucial to reduce the risk of this disease transmitting between animal populations and to humans. We produced a monoclonal antibody LPS-6B5, which shows high affinity to LPS and limited cross-reactivity with other bacteria. Based on LPS-6B5, a colloidal gold immunochromatographic assay (GICA) was developed which demonstrates high sensitivity and specificity in detecting cultured B. melitensis, B. abortus and B. suis. The Gold Immunochromatographic Assay (GICA) strips exhibited the most sensitive detection limits, with a value of 7.8125 × 10
Identifiants
pubmed: 39443541
doi: 10.1038/s41598-024-76026-4
pii: 10.1038/s41598-024-76026-4
doi:
Substances chimiques
Gold Colloid
0
Antibodies, Monoclonal
0
Reagent Strips
0
Lipopolysaccharides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
25068Subventions
Organisme : Postgraduate Research & Practice Innovation Program of Jiangsu Province
ID : KYCX23-2963
Organisme : Xuzhou Science and Technology Bureau
ID : KC23306
Organisme : Medical Research Program of Jiangsu Commission of Health
ID : Z2023080
Informations de copyright
© 2024. The Author(s).
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