Separation and purification of antimicrobial substances from Paenibacillus polymyxa KH-19 and analysis of its physicochemical characterization.
Paenibacillus polymyxa
Pectobacterium carotovorum BC2
Antimicrobial protein
Bioinformatics analysis
Isolation and purification
Protein structure prediction
Journal
Antonie van Leeuwenhoek
ISSN: 1572-9699
Titre abrégé: Antonie Van Leeuwenhoek
Pays: Netherlands
ID NLM: 0372625
Informations de publication
Date de publication:
24 Oct 2024
24 Oct 2024
Historique:
received:
20
02
2024
accepted:
01
10
2024
medline:
24
10
2024
pubmed:
24
10
2024
entrez:
24
10
2024
Statut:
epublish
Résumé
Soft rot is one of the top ten most dangerous plant pathogens in agricultural production, storage, and transport, and the use of microorganisms and their metabolites to control soft rot is a current research hotspot. In this study, we identified the antimicrobial substance in the metabolite of Paenibacillus polymyxa KH-19, and determined that the antimicrobial substance of this strain was an active protein. The protein was completely precipitated at 40-60% ammonium sulphate saturation and showed good inhibitory effects against seven pathogenic bacteria including Pectobacterium carotovorum BC2 and seven pathogenic fungi including Pyricularia oryzae. The MIC of the protein was 51.563 µg/mL, temperature acid-base UV and light stability insensitive to protease, with high-temperature resistance. The antimicrobial protein was isolated and purified by DEAE-anion exchange column and Sephadex G-75 gel filtration chromatography, and the LC-MS/MS assay identified the protein as lysophosphatidyl esterase with a molecular weight of 25.255 kDa. The purified antimicrobial protein increased the inhibitory effect against P. carotovorum BC2, with the diameter of the circle of inhibition being 26.50 ± 0.915 mm. Bioinformatics analysis showed that the protein has the molecular formula of C
Identifiants
pubmed: 39446216
doi: 10.1007/s10482-024-02029-w
pii: 10.1007/s10482-024-02029-w
doi:
Substances chimiques
Anti-Infective Agents
0
Bacterial Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
23Subventions
Organisme : Foundation Project of Heilongjiang Academy of Sciences
ID : KY2022SW03
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.
Références
Abdallah Y, Yang M, Zhang M, Masum MMI, Ogunyemi SO, Hossain A, An Q, Yan C, Li B (2019) Plant growth promotion and suppression of bacterial leaf blight in rice by Paenibacillus polymyxa Sx3. Lett Appl Microbiol 68(5):423–429
doi: 10.1111/lam.13117
pubmed: 30659625
Ash C, Priest FG, Collins MD (1994) Paenibacillus gen. nov. and Paenibacillus polymyxa comb. nov. IJSB 44:852–857
Belén CR, Nadia DRV, Cecilia MMT, Carlos HAA, Misael MB, María SVM (2021) Paenibacillus polymyxa NMA1017 as a potential biocontrol agent of Phytophthora tropicalis, causal agent of cacao black pod rot in Chiapas. Mexico Antonie Van Leeuwenhoek 114(1):55–68
doi: 10.1007/s10482-020-01498-z
Belén CR, Jeniffer CKD, Marí CAC, J AI, María SVM, Paulina ELS, (2020) Inhibition of Rhizoctonia solani RhCh-14 and Pythium ultimum PyFr-14 by Paenibacillus polymyxa NMA1017 and Burkholderia cenocepacia CACua-24: a proposal for biocontrol of phytopathogenic fungi. Microbiol Res 230:126347
doi: 10.1016/j.micres.2019.126347
Benjamin MZ, Christopher TS, Jonathan KO, Michael WWA, Robert MK (2018) A synthetic enzymatic pathway for extremely thermophilic acetone production based on the unexpectedly thermostable acetoacetate decarboxylase from Clostridium acetobutylicum. Biotechnol Bioeng 115(12):2951–2961
doi: 10.1002/bit.26829
Cao XF(2022)Lysophospholipids-underestimated molecules of the unique phospholipidome of Campylobacter jejuni.Utrecht University, Netherlands.
Cao X, Putten JPM, Wösten MMSM (2023) Biological functions of bacterial lysophospholipids. Adv Microb Physiol 82:129–154
doi: 10.1016/bs.ampbs.2022.10.001
pubmed: 36948653
Chen Xl, Hao ZB, Wang GH, Jin J, Liu JD (2007) Purification of antifungal protein from Paenibacillus polymyxa BRF-1. Chinese J Biol Control 23(2):156–159
Choi SK, Park SY, Kim R, Lee CH, Kim JYF, Park SH (2008) Identification and functional analysis of the fusaricidin biosynthetic gene of Paenibacillus polymyxa E681. Biochem Biophys Res Commun 365(1):89–95
doi: 10.1016/j.bbrc.2007.10.147
pubmed: 17980146
Czajkowski R, Pérombelon MCM, Veen JA, Wolf JM (2011) Control of blackleg and tuber soft rot of potato caused by Pectobacterium and Dickeya species: a review. Mol Plant Pathol 60:999–1013
doi: 10.1111/j.1365-3059.2011.02470.x
Davidsson PR, Kariola T, Niemi O, Palva ET (2013) Pathogenicity of and plant immunity to soft rot pectobacteria. Front Plant Sci 4:191
doi: 10.3389/fpls.2013.00191
pubmed: 23781227
pmcid: 3678301
Deng Y, Lu ZX, Lu FX, Zhang C, Wang Y, Zhao HZ, Bie XM (2011) Identification of LI-F type antibiotics and di-n-butyl phthalate produced by Paenibacillus polymyxa. J Microbiol Meth 85(3):175–182
doi: 10.1016/j.mimet.2011.02.013
Deng Y(2012)Identification of antimicrobial substances produced by Paenibacillus polymyxa JSa-9 and application in biocontrol of wheat. Nanjing Agricultual University (NanJing, China).
Fan RM, Zhang YW, Feng Y, Chen R, Sang XY, Jiang N (2023) Research progress of TatD-like DNase in Zoomotic parasites and pathogenic bacteria. Chin J Vet Sci 43(7):1582–1586
Fang ZD (1998) Research Methods for Plant Diseases, 3rd edn. Chinese Agriculture Press, Beijing, pp 248–249
Grady EN, Macdonald J, Liu L, Richman A, Yuan ZC (2016) Current knowledge and perspectives of Paenibacillus: a review. Microb Cell Fact 15(1):203. https://doi.org/10.1186/s12934-016-0603-7
doi: 10.1186/s12934-016-0603-7
pubmed: 27905924
pmcid: 5134293
Guo SS, Zhang JZ (2019) Research progress of Paenibacillus polymyxa and its lipopeptide compounds. Chinese J Pestic Sci 21(5–6):787–798
He X, Lu T, Zhou X (2021) Whole genome sequencing and comparative genomics analysis of Pectobacterium carotovorum identifies key pathogenic genes. Mol Phylogenet Evol 162:107–114
doi: 10.1016/j.ympev.2021.107114
Hossain A, Ali MA, Lin L, Luo J, You Y, Masum MMI, Jiang Y, Wang Y, Li B, An Q (2023) Biocontrol of soft rot dickeya and pectobacterium pathogens by broad-spectrum antagonistic bacteria within Paenibacillus polymyxa Complex. Microorganisms 11(4):817. https://doi.org/10.3390/microorganisms11040817
doi: 10.3390/microorganisms11040817
pubmed: 37110240
pmcid: 10142376
Irith W, Kai H, Hancock REW (2008) Agar and broth dilution methods to determine the minimal inhibitory concentration(MIC) of anitimicrobial substances. Nature Protocol 3(2):163–175
doi: 10.1038/nprot.2007.521
Jin MF, Ding KW, Lin MZ, Wu SQ, Su F (2020) The extraction and purification of the antifungal substances to the Fusarium oxysporum from the Paenibacillus polymyxa S960. J Anhui Agricul Univer 47(5):798–804
Khan Z, Kim SG, Jeon YH, Son SH, Kim YH (2008) A plant growth promoting rhizobacterium, Paenibacillus polymyxa strain GBR-1, suppresses root-knot nematode. Bioresour Technol 99(8):3016–3023
doi: 10.1016/j.biortech.2007.06.031
pubmed: 17706411
Li S, Zhao ZA, Li M, Gu ZR, Bai C, Huang WD (2002) Purification and characterization of a Novel Chitinase from Bacillus brevis. Acta Biochim Biophys Sin (Shanghai) 34(6):690–696
Lin MZ, Jin MF, Xu K, He LC, Cheng DL (2018) Phosphate-solubilizing bacteria improve the phytoremediation efficiency of Wedelia trilobata for Cu-contaminated soil. Int J Phytoremediation 20(8):813–822
doi: 10.1080/15226514.2018.1438351
pubmed: 29775097
Liu SD, Liu HM, Zhou L, Cheng ZG, Wan J, Pan Y, Xu G, Huang F, Wang M, Xiong YY, Hu GY (2022) Enhancement of antibacterial and growth-promoting effects of Paenibacillus polymyxa by optimizing its fermentation process. J Appl Microbiol 133:2954–2965
doi: 10.1111/jam.15750
pubmed: 35938320
Ma M, Wang C, Ding Y, Li L, Shen D, Jiang X, Guan D, Cao F, Chen H, Feng R, Wang X, Ge Y, Yao L, Bing X, Yang X, Li J, Du B (2011) Complete genome sequence of Paenibacillus polymyxa SC2, a strain of plant growth-promoting rhizobacterium with broad-spectrum antimicrobial activity. J Bacteriol 193(1):311–312
doi: 10.1128/JB.01234-10
pubmed: 21037012
Mansfield J, Genin S, Magori S, Citovsky V, Sriariyanum M, Ronald P, Dow M, Verdier V, Beer SV, Machado MA, Toth I, Salmond G, Foster GD (2012) Top 10 plant pathogenic bacteria in molecular plant pathology. Mol Plant Pathol 13:614–629
doi: 10.1111/j.1364-3703.2012.00804.x
pubmed: 22672649
pmcid: 6638704
Martin DF, Priest FG, Told C, Goodfellow M (1980) Distribution of beta-guconases within the genus Bacillus. Appl Environ Microbiol 40(6):1136–1138
doi: 10.1128/aem.40.6.1136-1138.1980
pubmed: 7458311
pmcid: 291733
Piuri M, Sanchez-Rivas C, Ruzal SM (1998) A novel antimicrobial activity of a Paenibacillus polymyxa strain isolated from regional fermented sausages. Lett Appl Microbiol 27(1):9–13
doi: 10.1046/j.1472-765X.1998.00374.x
pubmed: 9722991
Ran JJ, Jiao LX, Zhao RX, Zhu MM, Shi JR, Xu BC, Pan L (2021) Characterization of a novel antifungal protein produced by Paenibacillus polymyxa isolated from the wheat rhizosphere. J Sci Food Agric 101(5):1901–1909
doi: 10.1002/jsfa.10805
pubmed: 32895910
Ravi AV, Musthafa KS, Jegathammbal G, Kathiresan K, Pandian SK (2007) Screening and evaluation of probiotics as a biocontrol agent against pathogenic Vibrios in marine aquaculture. Lett Appl Microbiol 45(2):219–223
doi: 10.1111/j.1472-765X.2007.02180.x
pubmed: 17651222
Raza W, Yang W, Shen QR (2008) Paenibacillus polymyxa: antibiotics, hydrolytic enzymes and hazard assessment. J. Plant Pathol. 90:419–430
Samuel JH, Tanita W, Allen L, Benjamin RM, Julia S, Azita L, Erez Y, Rotem S, Philip JK (2022) Phage anti-CBASS and anti-Pycsar nucleases subvert bacterial immunity. Nature 605:522–543
doi: 10.1038/s41586-022-04716-y
Timmusk S, West P, Gow NAR, Huffstutler RP (2009) Paenibacillus polymyxa antagonizes oomycete plant pathogens Phytophthora palmivora and Pythium aphanidermatum. J Appl Microbiol 106(5):1473–1481
doi: 10.1111/j.1365-2672.2009.04123.x
pubmed: 19226403
Velkov T, Thompson PE, Nation RL, Li J (2010) Structure–activity elationships of polymyxin antibiotics. J Med Chem 53:1898–1916
doi: 10.1021/jm900999h
pubmed: 19874036
pmcid: 2907661
Vyacheslav SG, Kristina VT, Alexander AA, Elena NS, Alexander AS, Yulia PF, Irina VY (2020) Isolation, structure, and potential biotechnological applications of the exopolysaccharide from Paenibacillus polymyxa 92. Carbohydr Polym 15(232):115780
Wang GL, Wang XY, Song J, Wang HT, Ruan CF, Zhang WS, Guo Z, Li Guo WZ (2023) Cotton peroxisome-localized lysophospholipase counteracts the toxic effects of Verticillium dahliae NLP1 and confers wilt resistance. Plant J 115:452–469. https://doi.org/10.1111/tpj.16236
doi: 10.1111/tpj.16236
pubmed: 37026387
Wang JS, Xu JQ, Jin HY, Hou SH, Feng JW (2019) Isolation identification and drug resistance analysis of Proteus Teratosus. China Animal Husbandry & Veterinary Medicine 46(5):264–271 ((in Chinese))
Weselowski B, Nathoo N, Eastman AW, MacDonald J, Yuan ZC (2016) Isolation, identification and characterization of Paenibacillus polymyxa CR1 with potentials for biopesticide, biofertilization, biomass degradation and biofuel production. BMC Microbiol 16:1–10
doi: 10.1186/s12866-016-0860-y
Yi W, Chen C, Gan X (2022a) Active metabolites from the Endophyte Paenibacillus polymyxa Y-1 of dendrobium nobile for the control of rice bacterial diseases. Front Chem 10:1–11a
doi: 10.3389/fchem.2022.879724
Yi W, Chen C, Gan X (2022b) Polymyxin B
doi: 10.3389/fcimb.2022.866357
Yuan Y, Zhang XY, Zhao Y, Zhang H, Zhou YF, Gao J (2019) A novel PL9 pectate lyase from Paenibacillus polymyxa KF-1: cloning, expression, and its application in pectin degradation. Int J Molecul Sci 20:1–17
Zhai Y, Zhu JX, Tan TM, Xu JP, Shen AR, Yang XB, Li JL, Zeng LB, Wei L (2021) Isolation and characterization of antagonistic Paenibacillus polymyxa HX-140 and its biocontrol potential against Fusarium wilt of cucumber seedlings. BMC Microbiol 21(1):75
doi: 10.1186/s12866-021-02131-3
pubmed: 33676418
pmcid: 7936408
Zhao DL (2006) Primary Study of the Antifungal Activity of Bacillus polymyxa JW-725[D]. Sichuan University, Chengdu