Supercharged fluorescent proteins detect lanthanides via direct antennae signaling.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
24 Oct 2024
24 Oct 2024
Historique:
received:
30
08
2023
accepted:
28
09
2024
medline:
25
10
2024
pubmed:
25
10
2024
entrez:
25
10
2024
Statut:
epublish
Résumé
A sustainable operation for harvesting metals in the lanthanide series is needed to meet the rising demand for rare earth elements across diverse global industries. However, existing methods are limited in their capacity for detection and capture at environmentally and industrially relevant lanthanide concentrations. Supercharged fluorescent proteins have solvent-exposed, negatively charged residues that potentially create multiple direct chelation pockets for free lanthanide cations. Here, we demonstrate that negatively supercharged proteins can bind and quantitatively report concentrations of lanthanides via an underutilized lanthanide-to-chromophore pathway of energy transfer. The top-performing sensors detect lanthanides in the micromolar to millimolar range and remain unperturbed by environmentally significant concentrations of competing metals. As a demonstration of the versatility and adaptability of this energy transfer method, we show proximity and signal transmission between the lanthanides and a supramolecular assembly of supercharged proteins, paving the way for the detection of lanthanides via programmable protein oligomers and materials.
Identifiants
pubmed: 39448572
doi: 10.1038/s41467-024-53106-7
pii: 10.1038/s41467-024-53106-7
doi:
Substances chimiques
Lanthanoid Series Elements
0
Luminescent Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
9200Subventions
Organisme : United States Department of Defense | United States Army | US Army Corps of Engineers | Engineer Research and Development Center (U.S. Army Engineer Research and Development Center)
ID : W912HZ-22-Q-0016
Organisme : United States Department of Defense | United States Army | US Army Corps of Engineers | Engineer Research and Development Center (U.S. Army Engineer Research and Development Center)
ID : W912HZ-22-2-0037
Organisme : United States Department of Defense | United States Army | US Army Corps of Engineers | Engineer Research and Development Center (U.S. Army Engineer Research and Development Center)
ID : W912HZ-22-2-0037
Organisme : United States Department of Defense | United States Army | U.S. Army Research, Development and Engineering Command | Army Research Office (ARO)
ID : W911NF2220246
Organisme : United States Department of Defense | United States Army | U.S. Army Research, Development and Engineering Command | Army Research Office (ARO)
ID : W911NF2320089
Organisme : United States Department of Defense | United States Army | U.S. Army Research, Development and Engineering Command | Army Research Office (ARO)
ID : W911NF2220246
Informations de copyright
© 2024. The Author(s).
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