Epigenetic modulation via the C-terminal tail of H2A.Z.

Histones / metabolism Humans Nucleosomes / metabolism HeLa Cells Epigenesis, Genetic Heterochromatin / metabolism Animals Chromatin / metabolism Euchromatin / metabolism Cell Nucleus / metabolism

Journal

Nature communications

ISSN: 2041-1723

Titre abrégé: Nat Commun

Pays: England

ID NLM: 101528555

Informations de publication

Date de publication:
24 Oct 2024

Historique:

received: 08 07 2022

accepted: 12 10 2024

medline: 25 10 2024

pubmed: 25 10 2024

entrez: 25 10 2024

Statut: epublish

Résumé

H2A.Z-nucleosomes are present in both euchromatin and heterochromatin and it has proven difficult to interpret their disparate roles in the context of their stability features. Using an in situ assay of nucleosome stability and DT40 cells expressing engineered forms of the histone variant we show that native H2A.Z, but not C-terminally truncated H2A.Z (H2A.Z∆C), is released from nucleosomes of peripheral heterochromatin at unusually high salt concentrations. H2A.Z and H3K9me3 landscapes are reorganized in H2A.Z∆C-nuclei and overall sensitivity of chromatin to nucleases is increased. These tail-dependent differences are recapitulated upon treatment of HeLa nuclei with the H2A.Z-tail-peptide (C9), with MNase sensitivity being increased genome-wide. Fluorescence correlation spectroscopy revealed C9 binding to reconstituted nucleosomes. When introduced into live cells, C9 elicited chromatin reorganization, overall nucleosome destabilization and changes in gene expression. Thus, H2A.Z-nucleosomes influence global chromatin architecture in a tail-dependent manner, what can be modulated by introducing the tail-peptide into live cells.

Identifiants

DOI: 10.1038/s41467-024-53514-9 PMID: 39448645

pubmed: 39448645

doi: 10.1038/s41467-024-53514-9

pii: 10.1038/s41467-024-53514-9

doi:

Substances chimiques

Histones 0

Nucleosomes 0

Heterochromatin 0

Chromatin 0

histone H2A.F-Z 0

Euchromatin 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

9171

Subventions

Organisme : Nemzeti Kutatási, Fejlesztési és Innovációs Hivatal (NKFI Office)

ID : K138524, K128770

Organisme : European Cooperation in Science and Technology (COST)

ID : CA15214, CA18127

Organisme : EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020)

ID : no. 739593

Informations de copyright

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