Optimized partial freezing protocol enables 10-day storage of rat livers.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
25 Oct 2024
25 Oct 2024
Historique:
received:
14
06
2024
accepted:
16
10
2024
medline:
25
10
2024
pubmed:
25
10
2024
entrez:
25
10
2024
Statut:
epublish
Résumé
Preserving organs at subzero temperatures with halted metabolic activity holds the potential to prolong preservation and expand the donor organ pool for transplant. Our group recently introduced partial freezing, a novel approach in high-subzero storage at -15 °C, enabling 5-day storage of rodent livers through precise control over ice nucleation and unfrozen fraction. However, increased vascular resistance and tissue edema suggested a need for improvements to extend viable preservation. Here, we describe an optimized partial freezing protocol with key optimizations, including an increased concentration of polyethylene glycol (PEG) to enhance membrane stability while minimizing shear stress during cryoprotectant unloading with an acclimation period and a maintained osmotic balance through an increase in bovine serum albumin (BSA). These approaches ensured the viability during preservation and recovery processes, promoting liver function and ensuring optimal preservation. This was evidenced by increased oxygen consumption, decreased vascular resistance, and edema. Ultimately, we show that using the optimized protocol, livers can be stored for 10 days with comparable vascular resistance and lactate levels to 5 days, outperforming the viability of time-matched static cold stored (SCS) livers as the current gold standard. This study represents a significant advancement in expanding organ availability through prolonged preservation, thereby revolutionizing transplant medicine.
Identifiants
pubmed: 39448774
doi: 10.1038/s41598-024-76674-6
pii: 10.1038/s41598-024-76674-6
doi:
Substances chimiques
Cryoprotective Agents
0
Polyethylene Glycols
3WJQ0SDW1A
Serum Albumin, Bovine
27432CM55Q
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
25260Subventions
Organisme : NIH HHS
ID : R01DK096075
Pays : United States
Organisme : NIH HHS
ID : R01DK114506
Pays : United States
Organisme : NIH HHS
ID : K99/R00 HL1431149
Pays : United States
Organisme : NIH HHS
ID : R01DK096075
Pays : United States
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
ID : SNSF PZ00P3-185927
Informations de copyright
© 2024. The Author(s).
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