Nanoplastics: Immune Impact, Detection, and Internalization after Human Blood Exposure by Single-Cell Mass Cytometry.
CyTOF
biocompatibility
blood exposure
immune cells
pollution
safety
Journal
Advanced materials (Deerfield Beach, Fla.)
ISSN: 1521-4095
Titre abrégé: Adv Mater
Pays: Germany
ID NLM: 9885358
Informations de publication
Date de publication:
24 Oct 2024
24 Oct 2024
Historique:
revised:
07
10
2024
received:
06
09
2024
medline:
25
10
2024
pubmed:
25
10
2024
entrez:
25
10
2024
Statut:
aheadofprint
Résumé
The increasing exposure to nanoplastics (NPs) raises significant concerns for human health, primarily due to their potential bioaccumulative properties. While NPs have recently been detected in human blood, their interactions with specific immune cell subtypes and their impact on immune regulation remain unclear. In this proof-of-concept study, model palladium-doped polystyrene NPs (PS-Pd NPs) are utilized to enable single-cell mass cytometry (CyTOF) detection. The size-dependent impact of carboxylate polystyrene NPs (50-200 nm) is investigated across 15 primary immune cell subpopulations using CyTOF. By taking advantage of Pd-doping for detecting PS-Pd NPs, this work evaluates their impact on human immune-cells at the single-cell level following blood exposure. This work traces PS-Pd NPs in 37 primary immune-cell subpopulations from human blood, quantifying the palladium atom count per cell by CyTOF while simultaneously assessing the impact of PS-Pd NPs on cell viability, functionality, and uptake. These results demonstrate that NPs can interact with, interfere with, and translocate into several immune cell subpopulations after exposure. In vivo distribution experiments in mice further confirmed their accumulation in immune cells within the liver, blood, and spleen, particularly in monocytes, macrophages, and dendritic cells. These findings provide valuable insights into the impact of NPs on human health.
Identifiants
pubmed: 39449193
doi: 10.1002/adma.202413413
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2413413Subventions
Organisme : the European Union's Horizon 2020 Research and Innovation Programme under Marie Sklodowska-Curie
Organisme : Dipartimento di Biologia, Università degli Studi di Padova
ID : STARS Consolidator Grant (DETECTION)
Organisme : Erasmus+
ID : 2022-1-TR01-KA-VET-000088373
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
ID : PCEFP2_186856
Organisme : H2020 Marie Skłodowska-Curie Actions
ID : 101029140
Organisme : HORIZON EUROPE Marie Sklodowska-Curie Actions
ID : 101086184
Informations de copyright
© 2024 The Author(s). Advanced Materials published by Wiley‐VCH GmbH.
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