Whole-chromosome oligo-painting in licorice unveils interspecific chromosomal evolutionary relationships and possible origin of triploid genome species.
chromosomal evolutionary relationships
licorice
oligo‐FISH
origins
polyploid evolution
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
25 Oct 2024
25 Oct 2024
Historique:
revised:
24
09
2024
received:
01
05
2024
accepted:
12
10
2024
medline:
25
10
2024
pubmed:
25
10
2024
entrez:
25
10
2024
Statut:
aheadofprint
Résumé
Licorice is one of the most extensively studied medicinal plants in the world, whose roots and rhizomes have long been used as both a sweetener and an essential component in numerous herbal preparations. However, the genus Glycyrrhiza has a complex composition, and the interspecies chromosomal relationships, origin, and evolution are still largely unclear. Here, we develop a set of whole-chromosome painting probes that allowed identification of all eight chromosomes of licorice on same metaphase chromosomes. Comparative chromosome painting analyses in seven different Glycyrrhiza species revealed that the genus Glycyrrhiza maintained extraordinarily conserved chromosomal synteny after about 3-12 million years of divergence. No cytologically visible inter-chromosomal rearrangements were identified in any species. By comparative chromosomal karyotype analyses, we revealed interspecific chromosome evolutionary relationships and dramatic variable chromosomal karyotype after independent divergence and demonstrated that G. prostrate was the most closely related to the ancestral type among the seven Glycyrrhiza species. Furthermore, we also discovered a G. glandulosa seed with distinct triploid-genome for the first time in China, suggesting the existence of a polyploid evolutionary pathway in the genus Glycyrrhiza, which challenges the previous notion that only diploids of licorice existed in nature. This study expands our knowledge of the chromosome evolution of licorice and will lay an important foundation for the genome origin and evolution studies in the genus Glycyrrhiza.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Shihezi University Youth Top-notch Training Project
ID : BJZK202403
Organisme : Science and Technology Project of Bingtuan
ID : 2023AB052
Organisme : Science and Technology Project of Bingtuan
ID : 2023CB008
Organisme : Science and Technology Project of Bingtuan
ID : 2024DA021
Organisme : Tianchi Talent Project of Xinjiang
ID : CZ001604
Organisme : High level Talents Scientific Startup Project of Shihezi University
ID : RCZK202362
Organisme : Science and Technology Project of Huyanghe City
ID : QS2023008
Organisme : National Natural Science Foundation of China
ID : 32260083
Informations de copyright
© 2024 Society for Experimental Biology and John Wiley & Sons Ltd.
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