Structural basis for regulation of a CBASS-CRISPR-Cas defense island by a transmembrane anti-σ factor and its ECF σ partner.

CRISPR-Cas Systems Sigma Factor / metabolism Myxococcus xanthus / metabolism Bacterial Proteins / metabolism Models, Molecular Cryoelectron Microscopy Bacteriophages / metabolism Catalytic Domain Protein Multimerization

Journal

Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440

Informations de publication

Date de publication:
25 Oct 2024
Historique:
medline: 25 10 2024
pubmed: 25 10 2024
entrez: 25 10 2024
Statut: ppublish

Résumé

How CRISPR-Cas and cyclic oligonucleotide-based antiphage signaling systems (CBASS) are coordinately deployed against invaders remains unclear. We show that a locus containing two CBASS and one type III-B CRISPR-Cas system, regulated by the transmembrane anti-σ DdvA and its cognate extracytoplasmic function (ECF) σ DdvS, can defend

Identifiants

DOI: 10.1126/sciadv.adp1053 PMID: 39454004
pubmed: 39454004
doi: 10.1126/sciadv.adp1053
doi:

Substances chimiques

Sigma Factor 0
Bacterial Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

eadp1053

Auteurs

Diego Bernal-Bernal (D)

Departamento de Genética y Microbiología, Área de Genética (Unidad Asociada al IQF-CSIC), Universidad de Murcia, 30100 Murcia, Spain.
Instituto de Química Física "Blas Cabrera," CSIC (IQF-CSIC), 28006 Madrid, Spain.
Instituto Biofisika (UPV/EHU, CSIC), 48940 Leioa, Spain.
ORCID: 0009-0000-6518-6297

David Pantoja-Uceda (D)

Instituto de Química Física "Blas Cabrera," CSIC (IQF-CSIC), 28006 Madrid, Spain.
ORCID: 0000-0003-4390-6972

Jorge Pedro López-Alonso (JP)

Instituto Biofisika (UPV/EHU, CSIC), 48940 Leioa, Spain.
ORCID: 0000-0003-2081-2607

Alfonso López-Rojo (A)

Departamento de Genética y Microbiología, Área de Genética (Unidad Asociada al IQF-CSIC), Universidad de Murcia, 30100 Murcia, Spain.
ORCID: 0009-0009-8960-8699

José Antonio López-Ruiz (JA)

Departamento de Genética y Microbiología, Área de Genética (Unidad Asociada al IQF-CSIC), Universidad de Murcia, 30100 Murcia, Spain.

Marisa Galbis-Martínez (M)

Departamento de Genética y Microbiología, Área de Genética (Unidad Asociada al IQF-CSIC), Universidad de Murcia, 30100 Murcia, Spain.
ORCID: 0000-0003-1408-5298

Borja Ochoa-Lizarralde (B)

Instituto Biofisika (UPV/EHU, CSIC), 48940 Leioa, Spain.
ORCID: 0000-0002-5532-0218

Igor Tascón (I)

Instituto Biofisika (UPV/EHU, CSIC), 48940 Leioa, Spain.
Ikerbasque, Basque Foundation for Science, 48009 Bilbao, Spain.
ORCID: 0000-0003-2526-6238

Montserrat Elías-Arnanz (M)

Departamento de Genética y Microbiología, Área de Genética (Unidad Asociada al IQF-CSIC), Universidad de Murcia, 30100 Murcia, Spain.
ORCID: 0000-0003-1980-3092

Iban Ubarretxena-Belandia (I)

Instituto Biofisika (UPV/EHU, CSIC), 48940 Leioa, Spain.
Ikerbasque, Basque Foundation for Science, 48009 Bilbao, Spain.
ORCID: 0000-0003-4082-4349

S Padmanabhan (S)

Instituto de Química Física "Blas Cabrera," CSIC (IQF-CSIC), 28006 Madrid, Spain.
ORCID: 0000-0002-8772-0152

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Structural basis for regulation of a...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteur sigma › Structural basis for regulation of a...
questionsmedicales.fr › Bactéries › Proteobacteria › Deltaproteobacteria › Myxococcales › Myxococcus › Myxococcus xanthus › Structural basis for regulation of a...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Structural basis for regulation of a...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Structural basis for regulation of a...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › Structural basis for regulation of a...
questionsmedicales.fr › Virus › Bactériophages › Structural basis for regulation of a...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Éléments structuraux des protéines › Domaine catalytique › Structural basis for regulation of a...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Multimérisation de protéines › Structural basis for regulation of a...