Quantifying mitochondrial protein import by mePROD

Proteomics / methods Mitochondrial Proteins / metabolism Humans Isotope Labeling / methods Tandem Mass Spectrometry / methods Mitochondria / metabolism Protein Transport Chromatography, Liquid / methods
Mass spectrometry Mitochondria Mitochondrial protein import Proteomics SILAC TMT multiplex Translation mePROD pSILAC

Journal

Methods in enzymology
ISSN: 1557-7988
Titre abrégé: Methods Enzymol
Pays: United States
ID NLM: 0212271

Informations de publication

Date de publication:
2024
Historique:
medline: 26 10 2024
pubmed: 26 10 2024
entrez: 25 10 2024
Statut: ppublish

Résumé

Mitochondrial protein import is crucial for maintaining cellular health and homeostasis. Disruptions in this process have been linked to various diseases. Traditional methods for studying mitochondrial protein import predominantly focus on individual proteins and lack the dynamic resolution needed to fully appreciate the complexity of mitochondrial proteostasis and protein trafficking. To address these limitations, we developed a technique called mitochondria-specific multiplexed enhanced protein dynamics (mePROD

Identifiants

DOI: 10.1016/bs.mie.2024.07.017 PMID: 39455229
pubmed: 39455229
pii: S0076-6879(24)00347-1
doi: 10.1016/bs.mie.2024.07.017
pii:
doi:

Substances chimiques

Mitochondrial Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

449-474

Informations de copyright

Copyright © 2024. Published by Elsevier Inc.

Auteurs

Süleyman Bozkurt (S)

Institute of Molecular Systems Medicine, Faculty of Medicine, Goethe University, Frankfurt am Main, Germany.

Bhavesh S Parmar (BS)

Institute of Molecular Systems Medicine, Faculty of Medicine, Goethe University, Frankfurt am Main, Germany.

Christian Münch (C)

Institute of Molecular Systems Medicine, Faculty of Medicine, Goethe University, Frankfurt am Main, Germany; Cardio-Pulmonary Institute, Frankfurt am Main, Germany. Electronic address: ch.muench@em.uni-frankfurt.de.

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Classifications MeSH

questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Protéomique › Quantifying mitochondrial protein import by mePROD
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines mitochondriales › Quantifying mitochondrial protein import by mePROD
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Quantifying mitochondrial protein import by mePROD
questionsmedicales.fr › Techniques d'investigation › Marquage isotopique › Quantifying mitochondrial protein import by mePROD
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Quantifying mitochondrial protein import by mePROD
questionsmedicales.fr › Cellules › Structures cellulaires › Fractions subcellulaires › Mitochondries › Quantifying mitochondrial protein import by mePROD
questionsmedicales.fr › Métabolisme › Transport biologique › Transport des protéines › Quantifying mitochondrial protein import by mePROD
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Quantifying mitochondrial protein import by mePROD