Multi-omics profiling reveals the molecular mechanism of Bifidobacterium animalis BB04 in co-culture with Wickerhamomyces anomalus Y-5 to induce bifidocin A synthesis.


Journal

World journal of microbiology & biotechnology
ISSN: 1573-0972
Titre abrégé: World J Microbiol Biotechnol
Pays: Germany
ID NLM: 9012472

Informations de publication

Date de publication:
26 Oct 2024
Historique:
received: 29 08 2024
accepted: 16 10 2024
medline: 26 10 2024
pubmed: 26 10 2024
entrez: 25 10 2024
Statut: epublish

Résumé

Bacteriocin is a kind of natural substance that can effectively inhibit bacteria, but its production usually limited by environment. Co-culture is a strategy to stimulate bacteriocin production. Bifidocin A produced by Bifidobacterium animalis BB04, is a novel bacteriocin with a broad-spectrum antimicrobial active of foodborne bacteria. In order to enhance bifidocin A production, bacteriocin-inducing strains were screened firstly in co-cultivation. Then, the molecular mechanism of co-cultural induction was investigated by transcriptomic and proteomic analysis. Finally, the key inducing metabolites were identified by using targeted metabolomic technology. The results showed that Wickerhamomyces anomalus Y-5 in co-cultivation could significantly enhance bifidocin A production, with a 3.00-fold increase compared to mono-culture. The induction may not depend on direct contact with cells and may instead be attributed to be continuous exposure to inducing substances at specific concentration. In co-cultivation, W. anomalus Y-5 up-regulated Hxk2 and Tap42 to activate Glucose-cAMP and Tor and HOG-MAPK pathway, stimulated the expression of the retrograde gene, produced glutamine and glycerol to maintain activity. During this process, glutamine, inosine, guanosine, adenine, uracil, fumaric acid and pyruvic acid produced by W. anomalus Y-5 could induce the synthesis of bifidocin A. In conclusion, W. anomalus Y-5 in co-cultivation induced the synthesis of bifidocin A by regulating various signaling pathways to produce inducing substances. These findings establish a foundation for high-efficient synthesis of bifidocin A and provide a new perspective into the industrial production of bacteriocin.

Identifiants

pubmed: 39455466
doi: 10.1007/s11274-024-04172-y
pii: 10.1007/s11274-024-04172-y
doi:

Substances chimiques

Bacteriocins 0
Anti-Bacterial Agents 0
Bacterial Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

366

Subventions

Organisme : National Natural Science Foundation of China
ID : 31871772
Organisme : Basic and Applied Basic Research Foundation of Guangdong Province
ID : 2022A1515140021

Informations de copyright

© 2024. The Author(s), under exclusive licence to Springer Nature B.V.

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Auteurs

Yangshuo Liu (Y)

School of Food and Health, Beijing Technology and Business University, Beijing, 100048, China.

Rong Nie (R)

School of Food and Health, Beijing Technology and Business University, Beijing, 100048, China.

Kaisheng Shen (K)

School of Food and Health, Beijing Technology and Business University, Beijing, 100048, China.

Xinjie Diao (X)

School of Food and Health, Beijing Technology and Business University, Beijing, 100048, China.

Guorong Liu (G)

School of Food and Health, Beijing Technology and Business University, Beijing, 100048, China. liuguorong@th.btbu.edu.cn.
Key Laboratory of Geriatric Nutrition and Health, Ministry of Education, Beijing Technology and Business University, Beijing, 100048, China. liuguorong@th.btbu.edu.cn.
Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing, 100048, China. liuguorong@th.btbu.edu.cn.

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