Chemical composition of anti-microbially active fractions derived from extract of filamentous fungus Keratinophyton Lemmensii including three novel bioactive compounds.

Humans Cell Line, Tumor Anti-Infective Agents / pharmacology Microbial Sensitivity Tests Antineoplastic Agents / pharmacology Antioxidants / pharmacology
Fungal metabolites Keratinophilic fungi Okaramines

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
25 Oct 2024
Historique:
received: 06 08 2024
accepted: 07 10 2024
medline: 26 10 2024
pubmed: 26 10 2024
entrez: 25 10 2024
Statut: epublish

Résumé

Screening for new bioactive microbial metabolites, we found a novel okaramine derivative, for which we propose the trivial name lemmokaramine, as well as two already known okaramine congeners - okaramine H and okaramine J - responsible for antimicrobial activity of the recently described microscopic filamentous fungus, Keratinophyton lemmensii BiMM-F76 (= CCF 6359). In addition, two novel substances, a new cyclohexyl denominated lemmensihexol and a new tetrahydroxypyrane denominated lemmensipyrane, were purified and characterized. The compounds were isolated from the culture extract of the fungus grown on modified yeast extract sucrose medium by means of flash chromatography followed by preparative HPLC. The chemical structures were elucidated by NMR and LC-MS. The new okaramine (lemmokaramine) exerted antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts and fungi and anticancer activity against different mammalian cell lines (Caco-2, HCT116, HT29, SW480, MCM G1, and MCM DLN). Furthermore, we found a significant antioxidant effect of lemmokaramine following H

Identifiants

DOI: 10.1038/s41598-024-75510-1 PMID: 39455635
pubmed: 39455635
doi: 10.1038/s41598-024-75510-1
pii: 10.1038/s41598-024-75510-1
doi:

Substances chimiques

Anti-Infective Agents 0
Antineoplastic Agents 0
Antioxidants 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

25310

Informations de copyright

© 2024. The Author(s).

Références

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Auteurs

Roman Labuda (R)

Unit of Food Microbiology, Department for Farm Animals and Veterinary Public Health, Institute of Food Safety, Food Technology and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210, Vienna, Austria. roman.labuda@vetmeduni.ac.at.
Core Facility Bioactive Molecules, Screening and Analysis and Research Platform Bioactive Microbial Metabolites (BiMM), Konrad Lorenz Strasse 24, 3430, Tulln an der Donau, Austria. roman.labuda@vetmeduni.ac.at.

Markus Bacher (M)

Core Facility Bioactive Molecules, Screening and Analysis and Research Platform Bioactive Microbial Metabolites (BiMM), Konrad Lorenz Strasse 24, 3430, Tulln an der Donau, Austria.
Institute of Chemistry of Renewable Resources, Department of Chemistry, University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad Lorenz Strasse 24, 3430, Tulln an der Donau, Austria.

Thomas Rosenau (T)

Institute of Chemistry of Renewable Resources, Department of Chemistry, University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad Lorenz Strasse 24, 3430, Tulln an der Donau, Austria.
Johan Gadolin Process Chemistry Centre, Åbo Akademi University, Porthansgatan 3, 20500, Turku, Finland.

Hannes Gratzl (H)

Core Facility Bioactive Molecules, Screening and Analysis and Research Platform Bioactive Microbial Metabolites (BiMM), Konrad Lorenz Strasse 24, 3430, Tulln an der Donau, Austria.
Department of Agrobiotechnology (IFA-Tulln), Institute of Bioanalytics and Agro-Metabolomics, University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad Lorenz Strasse 20, 3430, Tulln an der Donau, Austria.

Maria Doppler (M)

Core Facility Bioactive Molecules, Screening and Analysis and Research Platform Bioactive Microbial Metabolites (BiMM), Konrad Lorenz Strasse 24, 3430, Tulln an der Donau, Austria.
Department of Agrobiotechnology (IFA-Tulln), Institute of Bioanalytics and Agro-Metabolomics, University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad Lorenz Strasse 20, 3430, Tulln an der Donau, Austria.

Sonja Hager (S)

Department for Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Waehringerstrasse 38, 1090, Vienna, Austria.

Doris Marko (D)

Department for Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Waehringerstrasse 38, 1090, Vienna, Austria.

Christoph Wiesner (C)

Department of Medical and Pharmaceutical Biotechnology, IMC University of Applied Sciences Krems AT, 3500, Krems, Austria.

Monika Očková (M)

Department of Medical and Pharmaceutical Biotechnology, IMC University of Applied Sciences Krems AT, 3500, Krems, Austria.

Nicole Ollinger (N)

FFoQSI - Austrian Competence Centre for Feed and Food Quality, Safety & Innovation, Stelzhamerstr. 23, 4600, Wels, Austria.

Martin Wagner (M)

Unit of Food Microbiology, Department for Farm Animals and Veterinary Public Health, Institute of Food Safety, Food Technology and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210, Vienna, Austria.

Christoph Schüller (C)

Core Facility Bioactive Molecules, Screening and Analysis and Research Platform Bioactive Microbial Metabolites (BiMM), Konrad Lorenz Strasse 24, 3430, Tulln an der Donau, Austria.
Department of Applied Genetics and Cell Biology, Institute of Microbial Genetics, University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad Lorenz Strasse 24, 3430, Tulln an der Donau, Austria.

Joseph Strauss (J)

Core Facility Bioactive Molecules, Screening and Analysis and Research Platform Bioactive Microbial Metabolites (BiMM), Konrad Lorenz Strasse 24, 3430, Tulln an der Donau, Austria. joseph.strauss@boku.ac.at.
Department of Applied Genetics and Cell Biology, Institute of Microbial Genetics, University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad Lorenz Strasse 24, 3430, Tulln an der Donau, Austria. joseph.strauss@boku.ac.at.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Chemical composition of anti-microbially...
questionsmedicales.fr › Cellules › Cellules cultivées › Cellules cancéreuses en culture › Lignée cellulaire tumorale › Chemical composition of anti-microbially...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Chemical composition of anti-microbially...
questionsmedicales.fr › Techniques d'investigation › Évaluation préclinique de médicament › Tests de sensibilité microbienne › Chemical composition of anti-microbially...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Antinéoplasiques › Chemical composition of anti-microbially...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Agents protecteurs › Antioxydants › Chemical composition of anti-microbially...