A comparative study of extraction free detection of HBV DNA using sodium dodecyl sulfate, N-lauroylsarcosine sodium salt, and sodium dodecyl benzene sulfonate.

Humans DNA, Viral / blood Sodium Dodecyl Sulfate / chemistry Hepatitis B virus / genetics Benzenesulfonates Hepatitis B / diagnosis Female Male Surface-Active Agents / chemistry Dried Blood Spot Testing / methods Middle Aged Hepatitis B Surface Antigens / blood
HBV DNA N-Lauroylsarcosine sodium salt Sodium dodecyl benzene sulfonate Sodium dodecyl sulfate Surfactant qPCR

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
26 Oct 2024
Historique:
received: 20 07 2024
accepted: 09 10 2024
medline: 26 10 2024
pubmed: 26 10 2024
entrez: 25 10 2024
Statut: epublish

Résumé

This study aimed to develop an extraction-free method for quantitative and qualitative detection of HBV DNA compared to traditional nucleic acid extraction. Paired serum and dried blood spot (DBS) samples from 67 HBsAg-positive and 67 HBsAg-negative individuals were included. Two samples with known HBV DNA titers (~ 10

Identifiants

DOI: 10.1038/s41598-024-75944-7 PMID: 39455809
pubmed: 39455809
doi: 10.1038/s41598-024-75944-7
pii: 10.1038/s41598-024-75944-7
doi:

Substances chimiques

DNA, Viral 0
Sodium Dodecyl Sulfate 368GB5141J
dodecylbenzenesulfonic acid 60NSK897G9
Benzenesulfonates 0
Surface-Active Agents 0
Hepatitis B Surface Antigens 0

Types de publication

Journal Article Comparative Study

Langues

eng

Sous-ensembles de citation

IM

Pagination

25442

Subventions

Organisme : Japan Society for the Promotion of Science (JSPS) Core-to-Core Program
ID : JPJSCCB20210010
Organisme : Japan Society for the Promotion of Science (JSPS) Core-to-Core Program
ID : JPJSCCB20240009
Organisme : Ministry of Health, Labour and Welfare of Japan
ID : JPMH19HC1001
Organisme : Ministry of Health, Labour and Welfare of Japan
ID : JPMH22HC1001
Organisme : Fund for the promotion of Joint International Research (Fostering Joint International Research-B)
ID : JP18KK0262

Informations de copyright

© 2024. The Author(s).

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Auteurs

Ko Ko (K)

Department of Epidemiology, Infectious Disease Control and Prevention, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami, Hiroshima, 734-8551, Japan.
Project Research Center for Epidemiology and Prevention of Viral Hepatitis and Hepatocellular Carcinoma, Hiroshima University, Hiroshima, Japan.

Lyubov Mikhailovna Lokteva (LM)

Department of Epidemiology, Infectious Disease Control and Prevention, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami, Hiroshima, 734-8551, Japan.
Research Institute of Virology, Tashkent, Uzbekistan.

Golda Ataa Akuffo (GA)

Department of Epidemiology, Infectious Disease Control and Prevention, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami, Hiroshima, 734-8551, Japan.
Project Research Center for Epidemiology and Prevention of Viral Hepatitis and Hepatocellular Carcinoma, Hiroshima University, Hiroshima, Japan.

Zayar Phyo (Z)

Department of Epidemiology, Infectious Disease Control and Prevention, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami, Hiroshima, 734-8551, Japan.
Project Research Center for Epidemiology and Prevention of Viral Hepatitis and Hepatocellular Carcinoma, Hiroshima University, Hiroshima, Japan.

Chanroth Chhoung (C)

Department of Epidemiology, Infectious Disease Control and Prevention, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami, Hiroshima, 734-8551, Japan.
Project Research Center for Epidemiology and Prevention of Viral Hepatitis and Hepatocellular Carcinoma, Hiroshima University, Hiroshima, Japan.

E Bunthen (E)

Department of Epidemiology, Infectious Disease Control and Prevention, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami, Hiroshima, 734-8551, Japan.
National Payment Certification Agency, National Social Protection Council, Ministry of Economic and Finance, Phnom Penh, Cambodia.

Serge Ouoba (S)

Department of Epidemiology, Infectious Disease Control and Prevention, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami, Hiroshima, 734-8551, Japan.
Unité de Recherche Clinique de Nanoro (URCN), Institut de Recherche en Science de La Santé (IRSS), Nanoro, Burkina Faso.

Aya Sugiyama (A)

Department of Epidemiology, Infectious Disease Control and Prevention, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami, Hiroshima, 734-8551, Japan.
Project Research Center for Epidemiology and Prevention of Viral Hepatitis and Hepatocellular Carcinoma, Hiroshima University, Hiroshima, Japan.

Tomoyuki Akita (T)

Department of Epidemiology, Infectious Disease Control and Prevention, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami, Hiroshima, 734-8551, Japan.
Project Research Center for Epidemiology and Prevention of Viral Hepatitis and Hepatocellular Carcinoma, Hiroshima University, Hiroshima, Japan.

Kim Rattana (K)

National Maternal and Child Health Center (NMCHC), Ministry of Health, Phnom Penh, Cambodia.

Ork Vichit (O)

National Immunization Program (NIP), Ministry of Health, Phnom Penh, Cambodia.

Kazuaki Takahashi (K)

Department of Epidemiology, Infectious Disease Control and Prevention, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami, Hiroshima, 734-8551, Japan.
Project Research Center for Epidemiology and Prevention of Viral Hepatitis and Hepatocellular Carcinoma, Hiroshima University, Hiroshima, Japan.

Junko Tanaka (J)

Department of Epidemiology, Infectious Disease Control and Prevention, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami, Hiroshima, 734-8551, Japan. jun-tanaka@hiroshima-u.ac.jp.
Project Research Center for Epidemiology and Prevention of Viral Hepatitis and Hepatocellular Carcinoma, Hiroshima University, Hiroshima, Japan. jun-tanaka@hiroshima-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › A comparative study of extraction free...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN viral › A comparative study of extraction free...
questionsmedicales.fr › Lipides › Alcools gras › Dodécan-1-ol › Dodécyl-sulfate de sodium › A comparative study of extraction free...
questionsmedicales.fr › Virus › Virus de l'hépatite › Hepadnaviridae › Orthohepadnavirus › Virus de l'hépatite B › A comparative study of extraction free...
questionsmedicales.fr › Composés chimiques organiques › Composés du soufre › Acides du soufre › Acides sulfoniques › Acides arènesulfoniques › Arènesulfonates › Benzènesulfonates › A comparative study of extraction free...
questionsmedicales.fr › Maladies de l'appareil digestif › Maladies du foie › Hépatite › Hépatites virales humaines › Hépatite B › A comparative study of extraction free...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Tensioactifs › A comparative study of extraction free...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Tests de chimie clinique › Analyse chimique du sang › Dépistage sur goutte de sang séché › A comparative study of extraction free...
questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Adulte d'âge moyen › A comparative study of extraction free...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Antigènes viraux › Antigènes d'hépatite virale › Antigènes de l'hépatite virale B › Antigènes de surface du virus de l'hépatite B › A comparative study of extraction free...