Serum antibodies against mimotopes of Merkel cell polyomavirus oncoproteins detected by a novel immunoassay in healthy individuals and Merkel cell carcinoma patients.
Humans
Merkel cell polyomavirus
/ immunology
Carcinoma, Merkel Cell
/ immunology
Antibodies, Viral
/ blood
Immunoassay
/ methods
Immunoglobulin G
/ blood
Sensitivity and Specificity
Oncogene Proteins, Viral
/ immunology
Aged
Middle Aged
Male
Female
Aged, 80 and over
ROC Curve
Polyomavirus Infections
/ immunology
Journal
Microbial biotechnology
ISSN: 1751-7915
Titre abrégé: Microb Biotechnol
Pays: United States
ID NLM: 101316335
Informations de publication
Date de publication:
Oct 2024
Oct 2024
Historique:
received:
07
05
2024
accepted:
08
07
2024
medline:
26
10
2024
pubmed:
26
10
2024
entrez:
26
10
2024
Statut:
ppublish
Résumé
Merkel cell polyomavirus (MCPyV) is the foremost causative factor of Merkel cell carcinoma (MCC), a rare yet highly aggressive skin cancer. Although the evaluation of circulating IgG antibodies against Merkel cell polyomavirus (MCPyV) LT/sT oncoproteins is clinically useful for MCC diagnosis/prognosis, a limited number of assays for identifying such antibodies have been developed. Herein, a novel indirect immunoassay with synthetic epitopes/mimotopes of MCPyV oncoproteins was computationally designed and experimentally validated on control sera and sera from healthy individuals and MCC patients. Upon computational design of five synthetic peptides, the performance of the immunoassay in detecting anti-oncoprotein IgGs in MCPyV-positive and -negative control sera was evaluated. The immunoassay was afterwards extended on sera from healthy individuals, and, for longitudinal analysis, MCC patients. Performance properties such as sensitivity and specificity and positive/negative predictive values were adequate. Receiver-operating characteristic (ROC) curves indicated that the areas under the curves (AUCs) were within the low/moderately accurate ranges. Immunoassay was repeatable, reproducible and accurate. As expected, the serum anti-oncoprotein IgG prevalence in healthy individuals was low (2%-5%). Anti-oncoprotein IgGs slightly increased when MCC patients experienced partial tumour remission and/or stable disease, compared to baseline. Our data indicate that the newly developed immunoassay is reliable for detecting circulating anti-oncoprotein IgGs both in healthy individuals and MCC patients.
Identifiants
pubmed: 39460382
doi: 10.1111/1751-7915.14536
doi:
Substances chimiques
Antibodies, Viral
0
Immunoglobulin G
0
Oncogene Proteins, Viral
0
Types de publication
Journal Article
Evaluation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14536Subventions
Organisme : Associazione Italiana per la Ricerca sul Cancro
ID : 21956
Organisme : Associazione Italiana per la Ricerca sul Cancro
ID : 26829
Organisme : Fondazione Umberto Veronesi
ID : 2023
Organisme : University of Ferrara, Fondo di Ateneo per la ricerca (FAR)
ID : 2021
Organisme : Bando Giovani anno 2022 per progetti di ricerca finanziati con il contributo 5 × 1000
ID : 2020
Informations de copyright
© 2024 The Author(s). Microbial Biotechnology published by John Wiley & Sons Ltd.
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