Considerations of growth factor and material use in bone tissue engineering using biodegradable scaffolds in vitro and in vivo.
Tissue Scaffolds
/ chemistry
Tissue Engineering
/ methods
Animals
Bone Morphogenetic Protein 2
/ metabolism
Osteogenesis
/ drug effects
Mice
Bone and Bones
/ metabolism
Polyesters
/ chemistry
Alkaline Phosphatase
/ metabolism
Humans
Biocompatible Materials
/ chemistry
Coated Materials, Biocompatible
/ chemistry
Animal models
Bioactive coating
Biomaterial
Bone tissue engineering
CAM assay
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
28 10 2024
28 10 2024
Historique:
received:
13
04
2024
accepted:
03
10
2024
medline:
29
10
2024
pubmed:
29
10
2024
entrez:
29
10
2024
Statut:
epublish
Résumé
Bone tissue engineering aims to harness materials to develop functional bone tissue to heal 'critical-sized' bone defects. This study examined a robust, coated poly(caprolactone) trimethacrylate (PCL-TMA) 3D-printable scaffold designed to augment bone formation. Following optimisation of the coatings, three bioactive coatings were examined, i) elastin-like polypeptide (ELP), ii) poly(ethyl acrylate) (PEA), fibronectin (FN) and bone morphogenetic protein-2 (BMP-2) applied sequentially (PEA/FN/BMP-2) and iii) both ELP and PEA/FN/BMP-2 coatings applied concurrently. The scaffold material was robust and showed biodegradability. The coatings demonstrated a significant (p < 0.05) osteogenic response in vitro in alkaline phosphatase gene upregulation and alkaline phosphatase production. The PCL-TMA scaffold and coatings supported angiogenesis and displayed excellent biocompatibility following evaluation on the chorioallantoic membrane assay. No significant (p < 0.05) heterotopic bone formed on the scaffolds within a murine subcutaneous implantation model, compared to the positive control of BMP-2 loaded collagen sponge following examination by micro-computed tomography or histology. The current studies demonstrate a range of innovative coated scaffold constructs with in vitro efficacy and clearly illustrate the importance of an appropriate in vivo environment to validate in vitro functionality prior to scale up and preclinical application.
Identifiants
pubmed: 39468149
doi: 10.1038/s41598-024-75198-3
pii: 10.1038/s41598-024-75198-3
doi:
Substances chimiques
Bone Morphogenetic Protein 2
0
Polyesters
0
Alkaline Phosphatase
EC 3.1.3.1
polycaprolactone
24980-41-4
Biocompatible Materials
0
Coated Materials, Biocompatible
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
25832Subventions
Organisme : UK Regenerative Medicine Platform
ID : MR/R015651/1
Organisme : UK Regenerative Medicine Platform
ID : MR/R015651/1
Organisme : UK Regenerative Medicine Platform
ID : MR/R015651/1
Organisme : UK Regenerative Medicine Platform
ID : MR/R015651/1
Organisme : UK Regenerative Medicine Platform
ID : MR/R015651/1
Organisme : UK Regenerative Medicine Platform
ID : MR/R015651/1
Organisme : UK Regenerative Medicine Platform
ID : MR/R015651/1
Organisme : UK Regenerative Medicine Platform
ID : MR/R015651/1
Organisme : UK Regenerative Medicine Platform
ID : MR/R015651/1
Organisme : UK Regenerative Medicine Platform
ID : MR/R015651/1
Organisme : UK Regenerative Medicine Platform
ID : MR/R015651/1
Organisme : UK Regenerative Medicine Platform
ID : MR/R015651/1
Organisme : UK Regenerative Medicine Platform
ID : MR/R015651/1
Organisme : ERC proof of concept
ID : MINGRAFT
Organisme : ERC proof of concept
ID : MINGRAFT
Organisme : National Institute for Health and Care Research
ID : NIHR133314
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P017711/1
Pays : United Kingdom
Informations de copyright
© 2024. The Author(s).
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