Vascular Stress Markers Following Inhalation of Heated Tobacco Products: A Study on Extracellular Vesicles.
Endothelial function
Extracellular vesicles
Heat not burn products
Heated tobacco products
Microvesicles
Nicotine
Journal
Cardiovascular toxicology
ISSN: 1559-0259
Titre abrégé: Cardiovasc Toxicol
Pays: United States
ID NLM: 101135818
Informations de publication
Date de publication:
29 Oct 2024
29 Oct 2024
Historique:
received:
22
06
2024
accepted:
18
10
2024
medline:
30
10
2024
pubmed:
30
10
2024
entrez:
30
10
2024
Statut:
aheadofprint
Résumé
The advent of heated tobacco products (HTPs) has introduced new variables in the study of nicotine delivery systems and their health implications. Amidst concerns over cardiovascular effects, this study aims to elucidate the acute impact of HTP inhalation on extracellular vesicles (EV) levels in young, healthy individuals. In this controlled, acute exposure study, 23 young, healthy volunteers were subjected to HTP inhalation. EV levels of endothelial and platelet origin were quantified through flow cytometry before and after exposure. Data analysis was performed using multiple measures ANOVA to assess changes in EV concentrations. Our findings reveal a significant increase in EVs of endothelial and platelet origin following short-term HTP inhalation with nicotine. Notably, no significant change was observed in leukocyte- and neutrophil-derived EVs. This increase in EVs suggests acute vascular stress, with peak levels observed 4 h post-exposure. The rise in endothelial and platelet-derived EVs aligns with documented responses to acute vascular injury, paralleling the effects seen with traditional cigarette and e-cigarette use. Despite HTPs being marketed as safer alternatives, our results indicate that nicotine-containing HTPs may still pose significant vascular risks. These findings contribute to the growing body of evidence cautioning against the perceived safety of HTPs and reinforce the importance of regulatory oversight and public health initiatives targeting nicotine delivery technologies. Trial Registrations: ClinicalTrials.gov ID: NCT04824495, registered 2021-01-07.
Identifiants
pubmed: 39472409
doi: 10.1007/s12012-024-09934-6
pii: 10.1007/s12012-024-09934-6
doi:
Banques de données
ClinicalTrials.gov
['NCT04824495']
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2024. The Author(s).
Références
Collaborators, G. B. D. T. (2021). Spatial, temporal, and demographic patterns in prevalence of smoking tobacco use and attributable disease burden in 204 countries and territories, 1990–2019: A systematic analysis from the Global Burden of Disease Study 2019. Lancet, 397, 2337–2360.
doi: 10.1016/S0140-6736(21)01169-7
REPORT FROM THE COMMISSION: on the establishment of a substantial change of circumstances for heated tobacco products in line with Directive 2014/40/EU. European Comission. Available from: https://eur-lex.europa.eu/legal-content/EN/TXT/HTML/?uri=CELEX:52022DC0279 . Accessed 220922.
Upadhyay, S., Rahman, M., Johanson, G., Palmberg, L. and Ganguly, K. (2023). Heated Tobacco Products: Insights into Composition and Toxicity. Toxics 11.
Davis, B., Williams, M., & Talbot, P. (2019). iQOS: Evidence of pyrolysis and release of a toxicant from plastic. Tobacco Control, 28, 34–41.
doi: 10.1136/tobaccocontrol-2017-054104
Auer, R., Concha-Lozano, N., Jacot-Sadowski, I., Cornuz, J., & Berthet, A. (2017). Heat-not-burn tobacco cigarettes: Smoke by any other name. JAMA Internal Medicine, 177, 1050–1052.
doi: 10.1001/jamainternmed.2017.1419
Bekki, K., Inaba, Y., Uchiyama, S., & Kunugita, N. (2017). Comparison of chemicals in mainstream smoke in heat-not-burn tobacco and combustion cigarettes. Journal of UOEH, 39, 201–207.
doi: 10.7888/juoeh.39.201
Mitova, M. I., Campelos, P. B., Goujon-Ginglinger, C. G., Maeder, S., Mottier, N., Rouget, E. G., Tharin, M., & Tricker, A. R. (2016). Comparison of the impact of the Tobacco Heating System 2.2 and a cigarette on indoor air quality. Regulatory Toxicology and Pharmacology : RTP, 80, 91–101.
doi: 10.1016/j.yrtph.2016.06.005
Koike, S., Sato, K., Sawa, M., Inaba, Y., Hattori, K., Nakadate, K., Ushiyama, A. and Ogasawara, Y. (2022). Exposure to Heated Tobacco Products Aerosol Causes Acute Stress Responses in the Lung of Mouse. Antioxidants (Basel) 11.
Husari, A., El-Harakeh, M., Shihadeh, A., Daou, M. A. Z., Bitar, H., Karaoghlanian, N., Zaatari, G., & El-Sabban, M. (2023). The substitution of fifty percent of combustible tobacco smoke exposure with either electronic cigarettes or heated tobacco products did not attenuate acute lung injury in an animal model. Nicotine & Tobacco Research, 25, 1361–1368.
doi: 10.1093/ntr/ntad045
Nabavizadeh, P., Liu, J., Havel, C. M., Ibrahim, S., Derakhshandeh, R., Jacob Iii, P., & Springer, M. L. (2018). Vascular endothelial function is impaired by aerosol from a single IQOS HeatStick to the same extent as by cigarette smoke. Tobacco Control, 27, s13–s19.
doi: 10.1136/tobaccocontrol-2018-054325
Yáñez-Mó, M., Siljander, P. R., Andreu, Z., Zavec, A. B., Borràs, F. E., Buzas, E. I., Buzas, K., Casal, E., Cappello, F., Carvalho, J., Colás, E., Cordeiro-da Silva, A., Fais, S., Falcon-Perez, J. M., Ghobrial, I. M., Giebel, B., Gimona, M., Graner, M., Gursel, I., … De Wever, O. (2015). Biological properties of extracellular vesicles and their physiological functions. J Extracell Vesicles, 4, 27066.
doi: 10.3402/jev.v4.27066
Wang, Z., Cai, W., Hu, S., Xia, Y., Wang, Y., Zhang, Q. and Chen, L. (2017). A Meta-Analysis of Circulating Microvesicles in Patients with Myocardial Infarction. Arq Bras Cardiol. 109: 0.
Huo, S., Kränkel, N., Nave, A. H., Sperber, P. S., Rohmann, J. L., Piper, S. K., Heuschmann, P. U., Landmesser, U., Endres, M., Siegerink, B., & Liman, T. G. (2021). Endothelial and Leukocyte-Derived Microvesicles and Cardiovascular Risk After Stroke: PROSCIS-B. Neurology, 96, e937–e946.
doi: 10.1212/WNL.0000000000011223
Yuan, Y., Maitusong, M., & Muyesai, N. (2020). Association of endothelial and red blood cell microparticles with acute myocardial infarction in Chinese: A retrospective study. Ann Palliat Med, 9, 1564–1570.
doi: 10.21037/apm-20-397
Chatterjee, V., Yang, X., Ma, Y., Wu, M. H., & Yuan, S. Y. (2020). Extracellular vesicles: New players in regulating vascular barrier function. American Journal of Physiology. Heart and Circulatory Physiology, 319, H1181-h1196.
doi: 10.1152/ajpheart.00579.2020
Mobarrez, F., Antoniewicz, L., Bosson, J. A., Kuhl, J., Pisetsky, D. S., & Lundback, M. (2014). The effects of smoking on levels of endothelial progenitor cells and microparticles in the blood of healthy volunteers. PLoS ONE, 9, e90314.
doi: 10.1371/journal.pone.0090314
Mobarrez, F., Antoniewicz, L., Hedman, L., Bosson, J.A. and Lundback, M. (2020). Electronic cigarettes containing nicotine increase endothelial and platelet derived extracellular vesicles in healthy volunteers. Atherosclerosis. https://doi.org/10.1016/j.atherosclerosis.2020.02.010
Lyytinen, G., Melnikov, G., Brynedal, A., Anesäter, E., Antoniewicz, L., Blomberg, A., Wallén, H., Bosson, J. A., Hedman, L., Tehrani, S., & Lundbäck, M. (2023). Use of heated tobacco products (IQOS) causes an acute increase in arterial stiffness and platelet thrombus formation. Atherosclerosis. https://doi.org/10.1016/j.atherosclerosis.2023.117335
doi: 10.1016/j.atherosclerosis.2023.117335
Peng, M., Liu, X., & Xu, G. (2020). Extracellular Vesicles as Messengers in Atherosclerosis. Journal of cardiovascular translational research, 13, 121–130.
doi: 10.1007/s12265-019-09923-z
Ferroni, P., Martini, F., Riondino, S., La Farina, F., Magnapera, A., Ciatti, F., & Guadagni, F. (2009). Soluble P-selectin as a marker of in vivo platelet activation. Clinica Chimica Acta, 399, 88–91.
doi: 10.1016/j.cca.2008.09.018
Ishida, M., Sakai, C., Kobayashi, Y., & Ishida, T. (2024). Cigarette smoking and atherosclerotic cardiovascular disease. Journal of Atherosclerosis and Thrombosis, 31, 189–200.
doi: 10.5551/jat.RV22015
Benedikter, B.J., Wouters, E.F.M., Savelkoul, P.H.M., Rohde, G.G.U. and Stassen, F.R.M. (2018). Extracellular vesicles released in response to respiratory exposures: implications for chronic disease. Journal of Toxicology and Environmental Health Part B. 21: 142-160.
Strulovici-Barel, Y., Staudt, M. R., Krause, A., Gordon, C., Tilley, A. E., Harvey, B. G., Kaner, R. J., Hollmann, C., Mezey, J. G., Bitter, H., Pillai, S. G., Hilton, H., Wolff, G., Stevenson, C. S., Visvanathan, S., Fine, J. S., & Crystal, R. G. (2016). Persistence of circulating endothelial microparticles in COPD despite smoking cessation. Thorax, 71, 1137–1144.
doi: 10.1136/thoraxjnl-2015-208274
Antoniewicz, L., Bosson, J. A., Kuhl, J., Abdel-Halim, S. M., Kiessling, A., Mobarrez, F., & Lundback, M. (2016). Electronic cigarettes increase endothelial progenitor cells in the blood of healthy volunteers. Atherosclerosis, 255, 179–185.
doi: 10.1016/j.atherosclerosis.2016.09.064
Badrnya, S., Baumgartner, R., & Assinger, A. (2014). Smoking alters circulating plasma microvesicle pattern and microRNA signatures. Thrombosis and haemostasis, 112, 128–136.
doi: 10.1160/TH13-11-0977
Daiber, A., Kuntic, M., Oelze, M., Hahad, O., & Münzel, T. (2023). E-cigarette effects on vascular function in animals and humans. Pflugers Archiv. European Journal of Physiology, 475, 783–796.
doi: 10.1007/s00424-023-02813-z
Calenic, B., Miricescu, D., Greabu, M., Kuznetsov, A.V., Troppmair, J., Ruzsanyi, V. and Amann, A. (2015). Oxidative stress and volatile organic compounds: interplay in pulmonary, cardio-vascular, digestive tract systems and cancer. Open Chemistry 13.
Rahmandar, M. H., & Gribben, V. (2022). E-cigarette disparities: Who are the targets? Current Problems in Pediatric and Adolescent Health Care, 52, 101201.
doi: 10.1016/j.cppeds.2022.101201
Fu, X., Zong, T., Yang, P., Li, L., Wang, S., Wang, Z., Li, M., Li, X., Zou, Y., Zhang, Y., Htet Aung, L. H., Yang, Y., & Yu, T. (2021). Nicotine: Regulatory roles and mechanisms in atherosclerosis progression. Food and chemical toxicology : An international journal published for the British Industrial Biological Research Association, 151, 112154.
doi: 10.1016/j.fct.2021.112154
Anfossi, G., & Trovati, M. (1996). Role of catecholamines in platelet function: Pathophysiological and clinical significance. European journal of clinical investigation, 26, 353–370.
doi: 10.1046/j.1365-2362.1996.150293.x
Yao, Y., Mao, J., Xu, S., Zhao, L., Long, L., Chen, L., Li, D., & Lu, S. (2019). Rosmarinic acid inhibits nicotine-induced C-reactive protein generation by inhibiting NLRP3 inflammasome activation in smooth muscle cells. Journal of Cellular Physiology, 234, 1758–1767.
doi: 10.1002/jcp.27046
Wu, X., Zhang, H., Qi, W., Zhang, Y., Li, J., Li, Z., Lin, Y., Bai, X., Liu, X., Chen, X., Yang, H., Xu, C., Zhang, Y., & Yang, B. (2018). Nicotine promotes atherosclerosis via ROS-NLRP3-mediated endothelial cell pyroptosis. Cell Death & Disease, 9, 171.
doi: 10.1038/s41419-017-0257-3
Bodega, G., Alique, M., Puebla, L., Carracedo, J., & Ramírez, R. M. (2019). Microvesicles: ROS scavengers and ROS producers. J Extracell Vesicles, 8, 1626654.
doi: 10.1080/20013078.2019.1626654
Cardenas, H. L., Evanoff, N. G., Fandl, H. K., Berry, A. R., Wegerson, K. N., Ostrander, E. I., Greiner, J. J., Dufresne, S. R., Kotlyar, M., Dengel, D. R., DeSouza, C. A., & Garcia, V. P. (2023). Endothelial-derived extracellular vesicles associated with electronic cigarette use impair cerebral microvascular cell function. Journal of Applied Physiology, 135, 271–278.
doi: 10.1152/japplphysiol.00243.2023
Lyytinen, G., Brynedal, A., Anesäter, E., Antoniewicz, L., Blomberg, A., Wallén, H., Bosson, J. A., Hedman, L., Mobarrez, F., Tehrani, S., & Lundbäck, M. (2023). Electronic cigarette vaping with nicotine causes increased thrombogenicity and impaired microvascular function in healthy volunteers: A randomised clinical trial. Cardiovascular Toxicology, 23, 255–264.
doi: 10.1007/s12012-023-09802-9
Li, W., Du, D. Y., Liu, Y., Jiang, F., Zhang, P., & Li, Y. T. (2017). Long-term nicotine exposure induces dysfunction of mouse endothelial progenitor cells. Experimental and therapeutic medicine, 13, 85–90.
doi: 10.3892/etm.2016.3916
Zainalabidin, S., Budin, S. B., Ramalingam, A., & Lim, Y. C. (2014). Aortic remodelling in chronic nicotine-administered rat. Korean J Physiol Pharmacol, 18, 411–418.
doi: 10.4196/kjpp.2014.18.5.411
Arefalk, G., Hambraeus, K., Lind, L., Michaelsson, K., Lindahl, B., & Sundstrom, J. (2014). Discontinuation of smokeless tobacco and mortality risk after myocardial infarction. Circulation, 130, 325–332.
doi: 10.1161/CIRCULATIONAHA.113.007252
Byhamre, M. L., Araghi, M., Alfredsson, L., Bellocco, R., Engström, G., Eriksson, M., Galanti, M. R., Jansson, J. H., Lager, A., Lundberg, M., Östergren, P. O., Pedersen, N. L., Trolle Lagerros, Y., Ye, W., Wennberg, P., & Magnusson, C. (2021). Swedish snus use is associated with mortality: A pooled analysis of eight prospective studies. International journal of epidemiology, 49, 2041–2050.
doi: 10.1093/ije/dyaa197
Antoniewicz, L., Kabele, M., Nilsson, U., Pourazar, J., Rankin, G., Bosson, J. A., & Lundbäck, M. (2022). Chronic snus use in healthy males alters endothelial function and increases arterial stiffness. PLoS ONE, 17, e0268746.
doi: 10.1371/journal.pone.0268746
Joseph, A. M., Norman, S. M., Ferry, L. H., Prochazka, A. V., Westman, E. C., Steele, B. G., Sherman, S. E., Cleveland, M., Antonuccio, D. O., Hartman, N., & McGovern, P. G. (1996). The safety of transdermal nicotine as an aid to smoking cessation in patients with cardiac disease. The New England journal of medicine, 335, 1792–1798.
doi: 10.1056/NEJM199612123352402
Woolf, K. J., Zabad, M. N., Post, J. M., McNitt, S., Williams, G. C., & Bisognano, J. D. (2012). Effect of nicotine replacement therapy on cardiovascular outcomes after acute coronary syndromes. American Journal of Cardiology, 110, 968–970.
doi: 10.1016/j.amjcard.2012.05.028
Benowitz, N.L., Hukkanen, J. and Jacob, P., 3rd. (2009). Nicotine chemistry, metabolism, kinetics and biomarkers. Handbook of experimental pharmacology: 29–60.
Li, J., Liu, F., Liang, F., Yang, Y., Lu, X., & Gu, D. (2023). Air pollution exposure and vascular endothelial function: A systematic review and meta-analysis. Environmental Science and Pollution Research International, 30, 28525–28549.
doi: 10.1007/s11356-023-25156-9