Non-invasive SARS-CoV-2 RNA detection and human transcriptome analysis using skin surface lipids.

Humans SARS-CoV-2 / genetics COVID-19 / genetics RNA, Viral / genetics Skin / metabolism Gene Expression Profiling / methods Female Male Middle Aged Adult Lipids Aged Transcriptome Ubiquitins / genetics Myxovirus Resistance Proteins / genetics Cytokines

COVID-19 SARS-CoV-2 Sebum Skin Skin surface lipid Transcriptome

Journal

Scientific reports

ISSN: 2045-2322

Titre abrégé: Sci Rep

Pays: England

ID NLM: 101563288

Informations de publication

Date de publication:
30 10 2024

Historique:

received: 14 06 2024

accepted: 25 10 2024

medline: 30 10 2024

pubmed: 30 10 2024

entrez: 30 10 2024

Statut: epublish

Résumé

There have been several reports of skin manifestations in patients with coronavirus disease 2019 (COVID-19). However, it is unclear whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA can be detected on the skin surface, including the sebum, of these patients. In this study, SARS-CoV-2 RNA was detected using real-time reverse-transcription polymerase chain reaction (RT-PCR) assay of skin surface lipids (SSLs) collected using an oil-blotting film from the faces of hospitalized patients with COVID-19. Human transcriptome analysis was also performed using the same samples. In facial SSLs of patients with COVID-19, the RT-PCR positivity rate was 84.6% (11/13 samples) within 5 days and 30.4% (7/23 samples) by 6-10 days of symptom onset. In the transcriptome analysis, the most characteristic SSL-RNA profile was the upregulation of interferon-stimulated gene (ISG)-related genes, such as ISG15, IFITM1, and MX1. This study presents an alternative technique using SSLs for non-invasive SARS-CoV-2 RNA detection and simultaneous analysis of human molecular pathogenesis in patients with COVID-19.

Identifiants

DOI: 10.1038/s41598-024-77862-0 PMID: 39472469

pubmed: 39472469

doi: 10.1038/s41598-024-77862-0

pii: 10.1038/s41598-024-77862-0

doi:

Substances chimiques

RNA, Viral 0

Lipids 0

ISG15 protein, human 60267-61-0

Ubiquitins 0

Myxovirus Resistance Proteins 0

Cytokines 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

26057

Subventions

Organisme : Japan Agency for Medical Research and Development

ID : JP21fk0108104

Informations de copyright

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