Structure of the turnover-ready state of an ancestral respiratory complex I.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
29 Oct 2024
29 Oct 2024
Historique:
received:
19
08
2024
accepted:
21
10
2024
medline:
30
10
2024
pubmed:
30
10
2024
entrez:
30
10
2024
Statut:
epublish
Résumé
Respiratory complex I is pivotal for cellular energy conversion, harnessing energy from NADH:ubiquinone oxidoreduction to drive protons across energy-transducing membranes for ATP synthesis. Despite detailed structural information on complex I, its mechanism of catalysis remains elusive due to lack of accompanying functional data for comprehensive structure-function analyses. Here, we present the 2.3-Å resolution structure of complex I from the α-proteobacterium Paracoccus denitrificans, a close relative of the mitochondrial progenitor, in phospholipid-bilayer nanodiscs. Three eukaryotic-type supernumerary subunits (NDUFS4, NDUFS6 and NDUFA12) plus a novel L-isoaspartyl-O-methyltransferase are bound to the core complex. Importantly, the enzyme is in a single, homogeneous resting state that matches the closed, turnover-ready (active) state of mammalian complex I. Our structure reveals the elements that stabilise the closed state and completes P. denitrificans complex I as a unified platform for combining structure, function and genetics in mechanistic studies.
Identifiants
pubmed: 39472559
doi: 10.1038/s41467-024-53679-3
pii: 10.1038/s41467-024-53679-3
doi:
Substances chimiques
Electron Transport Complex I
EC 7.1.1.2
Bacterial Proteins
0
Protein Subunits
0
Methyltransferases
EC 2.1.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
9340Subventions
Organisme : RCUK | Medical Research Council (MRC)
ID : MC_UU_00015/2
Organisme : RCUK | Medical Research Council (MRC)
ID : MC_UU_00015/2
Organisme : RCUK | Medical Research Council (MRC)
ID : MC_UU_00015/2
Organisme : RCUK | Medical Research Council (MRC)
ID : MC_UU_00015/2
Informations de copyright
© 2024. The Author(s).
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