Ethylamine, beyond the synthetic precursor of theanine: CsCBF4-CsAlaDC module promoted ethylamine synthesis to enhance osmotic tolerance in tea plants.
CBF family
CsAlaDC
EA
ROS scavenger
osmotic stress
tea plants
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
30 Oct 2024
30 Oct 2024
Historique:
revised:
30
09
2024
received:
14
03
2024
accepted:
07
10
2024
medline:
30
10
2024
pubmed:
30
10
2024
entrez:
30
10
2024
Statut:
aheadofprint
Résumé
The tea plant (Camellia sinensis) is a perennial green plant, and its tender leaves are rich in secondary metabolites, such as theanine. Ethylamine (EA), a small amine, is an important prerequisite for theanine synthesis. However, beyond its involvement in theanine synthesis, the other physiological functions of EA in tea plants remain unknown. In vitro experiments indicate that EA may function as scavengers of reactive oxygen species (ROS) to protect the plant against damage caused by osmotic stress. Additionally, a significant correlation between EA levels and osmotic tolerance has been observed in different tea varieties. From the results, alanine decarboxylase (CsAlaDC)-silenced tea leaves and overexpressed CsAlaDC Arabidopsis thaliana lines decreased and increased EA levels, respectively, and mediated ROS homeostasis, thus exhibiting a sensitive and tolerant phenotype. In addition, the transcription factor (TF) CsCBF4 was functionally identified, which can directly bind to the CsAlaDC promoter. CsCBF4-silenced tea leaves significantly reduced the expression levels of CsAlaDC and in turn EA content, resulting in excess ROS accumulation and an osmotic-sensitive phenotype. Taken together, these results established a new regulatory module consisting of CBF4-CsAlaDC responsible for EA accumulation and ROS homeostasis in response to osmotic stress.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Science Foundation for Anhui Province
ID : 2022AH050919
Organisme : Key Research and Development Projects of Shaanxi Province
ID : 2017TSCXL-NY-02-02
Organisme : Scientific Research Project of Anhui Provincial Colleges and Universities
ID : 2023AH040136
Organisme : Natural Science Foundation of Anhui Province
ID : 2008085MC97
Organisme : Anhui Province Science and Technology Major Project
ID : 202203a06020014
Organisme : National Key Research and Development Program of China
ID : 2021YFD1601103
Informations de copyright
© 2024 Society for Experimental Biology and John Wiley & Sons Ltd.
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