First comparative genomics analysis of Corynebacterium auriscanis.
Journal
Memorias do Instituto Oswaldo Cruz
ISSN: 1678-8060
Titre abrégé: Mem Inst Oswaldo Cruz
Pays: Brazil
ID NLM: 7502619
Informations de publication
Date de publication:
2024
2024
Historique:
received:
15
07
2024
accepted:
05
09
2024
medline:
31
10
2024
pubmed:
30
10
2024
entrez:
30
10
2024
Statut:
epublish
Résumé
Corynebacterium auriscanis is a bacterial species frequently isolated from dogs with external otitis or dermatitis and a zoonotic pathogen transmitted by dog bite. It is considered an opportunistic pathogen, but its pathogenicity mechanisms are poorly studied. Comparative genomics can identify virulence and niche factors that could contribute to understanding its lifestyle. The objectives of this project was to compare genomes of C. auriscanis to identify genes related to its virulence and lifestyle. The genome of strain 32 was sequenced using Illumina HiSeq 2500 (Illumina, CA, USA) and assembled using Unicycler. The two other non-redundant genomes from the same species available in GenBank were included in the analysis. All genomes were annotated and checked for taxonomy, assembly quality, mobile elements, CRISPR-Cas systems, and virulence and antimicrobial resistance genes. The virulence genes in the three genomes were compared to the ones from other pathogens commonly isolated with C. auriscanis. The species has 42 virulence factors that can be classified as niche factors, due to the absence of true virulence factors found in primary pathogens. The gene rbpA could confer basal levels of resistance to rifampin. The absence of true virulence factors in the three genomes suggests C. auriscanis has an opportunistic pathogen lifestyle.
Sections du résumé
BACKGROUND
BACKGROUND
Corynebacterium auriscanis is a bacterial species frequently isolated from dogs with external otitis or dermatitis and a zoonotic pathogen transmitted by dog bite. It is considered an opportunistic pathogen, but its pathogenicity mechanisms are poorly studied. Comparative genomics can identify virulence and niche factors that could contribute to understanding its lifestyle.
OBJECTIVES
OBJECTIVE
The objectives of this project was to compare genomes of C. auriscanis to identify genes related to its virulence and lifestyle.
METHODS
METHODS
The genome of strain 32 was sequenced using Illumina HiSeq 2500 (Illumina, CA, USA) and assembled using Unicycler. The two other non-redundant genomes from the same species available in GenBank were included in the analysis. All genomes were annotated and checked for taxonomy, assembly quality, mobile elements, CRISPR-Cas systems, and virulence and antimicrobial resistance genes. The virulence genes in the three genomes were compared to the ones from other pathogens commonly isolated with C. auriscanis.
FINDINGS
RESULTS
The species has 42 virulence factors that can be classified as niche factors, due to the absence of true virulence factors found in primary pathogens. The gene rbpA could confer basal levels of resistance to rifampin.
MAIN CONCLUSIONS
CONCLUSIONS
The absence of true virulence factors in the three genomes suggests C. auriscanis has an opportunistic pathogen lifestyle.
Identifiants
pubmed: 39476150
pii: S0074-02762024000101132
doi: 10.1590/0074-02760240156
pii:
doi:
Substances chimiques
Virulence Factors
0
Types de publication
Journal Article
Comparative Study
Langues
eng
Sous-ensembles de citation
IM