Community composition and seasonal dynamics of microplastic biota in the Eastern Mediterranean Sea.
16S
18S
Biodiversity
Diatoms
Eastern Mediterranean Sea
Metabarcoding
Microbiome
Microplastic
Nanopore
Plastisphere
Seasonality
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
30 10 2024
30 10 2024
Historique:
received:
23
06
2024
accepted:
16
09
2024
medline:
31
10
2024
pubmed:
31
10
2024
entrez:
31
10
2024
Statut:
epublish
Résumé
Marine plastic pollution poses a growing environmental threat, with microplastics accumulating in the global oceans. This study profiles the seasonal dynamics and taxonomic composition of the plastisphere, the microplastic ecosystem, in the Eastern Mediterranean Sea. Using long-read 16 S and 18 S metabarcoding, we analyzed offshore microplastic and whole seawater samples across each season over a two-year period. The analysis revealed a higher richness of prokaryotic communities on microplastics compared to seawater, which was predominantly composed of Proteobacteria and Bacteroidota and exhibited notable seasonal variability. Benthic eukaryotes were enriched on microplastics compared to the surrounding seawater. Diatoms (Bacillariophyceae), in particular, showed significant enrichment within the microplastic eukaryotic community with primarily pennate diatoms of Amphora, Navicula, and Nitzschia genera, whereas the seawater included mostly centric diatoms. Seasonal fluctuations were less pronounced in the microplastic communities than in seawater, highlighting the relative stability of this new human-made ecosystem. These findings underscore the unique ecological niche of microplastic-associated communities in marine environments.
Identifiants
pubmed: 39477997
doi: 10.1038/s41598-024-73281-3
pii: 10.1038/s41598-024-73281-3
doi:
Substances chimiques
Microplastics
0
Water Pollutants, Chemical
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
26131Subventions
Organisme : Ministry of Science and Technology, Israel
ID : 3-1650
Informations de copyright
© 2024. The Author(s).
Références
Borrelle, S. B. et al. Predicted growth in plastic waste exceeds efforts to mitigate plastic pollution. Science 369, 1515–1518 (2020).
pubmed: 32943526
doi: 10.1126/science.aba3656
Lincoln, S. et al. Marine litter and climate change: inextricably connected threats to the world’s oceans. Sci. Total Environ. 837, 155709 (2022).
pubmed: 35525371
doi: 10.1016/j.scitotenv.2022.155709
Frias, J. P., Nash, R. & Microplastics Finding a consensus on the definition. Mar. Pollut. Bull. 138, 145–147 (2019).
pubmed: 30660255
doi: 10.1016/j.marpolbul.2018.11.022
Shahul Hamid, F. et al. Worldwide distribution and abundance of microplastic: how dire is the situation? Waste Manag. Res. 36, 873–897 (2018).
pubmed: 30103651
doi: 10.1177/0734242X18785730
Chen, B. et al. Global distribution of marine microplastics and potential for biodegradation. J. Hazard. Mater. 451, 131198 (2023).
pubmed: 36921415
doi: 10.1016/j.jhazmat.2023.131198
Cózar, A. et al. Plastic debris in the open ocean. Proc. Natl. Acad. Sci. 111, 10239–10244 (2014).
pubmed: 24982135
pmcid: 4104848
doi: 10.1073/pnas.1314705111
Hale, R. C., Seeley, M. E., Guardia, L., Mai, M. J. & Zeng, E. L. Y. A global perspective on microplastics. J. Geophys. Res. Oceans 125, e2018JC014719 (2020).
Marsay, K. S. et al. High-resolution screening for marine prokaryotes and eukaryotes with selective preference for polyethylene and polyethylene terephthalate surfaces. Front. Microbiol. 13, 845144 (2022).
pubmed: 35495680
pmcid: 9042255
doi: 10.3389/fmicb.2022.845144
Zettler, E. R., Mincer, T. J. & Amaral-Zettler, L. A. Life in the plastisphere: microbial communities on plastic marine debris. Environ. Sci. Technol. 47, 7137–7146 (2013).
pubmed: 23745679
doi: 10.1021/es401288x
Amaral-Zettler, L. A., Zettler, E. R. & Mincer, T. J. Ecology of the plastisphere. Nat. Rev. Microbiol. 18, 139–151 (2020).
pubmed: 31937947
doi: 10.1038/s41579-019-0308-0
Wang, Z. et al. Plastisphere enrich antibiotic resistance genes and potential pathogenic bacteria in sewage with pharmaceuticals. Sci. Total Environ. 768, 144663 (2021).
pubmed: 33454495
doi: 10.1016/j.scitotenv.2020.144663
Marsay, K. S. et al. The geographical and seasonal effects on the composition of marine microplastic and its microbial communities: the case study of Israel and Portugal. Front. Microbiol. 14, 1089926 (2023).
pubmed: 36910177
doi: 10.3389/fmicb.2023.1089926
Lv, S., Li, Y., Zhao, S. & Shao, Z. Biodegradation of typical plastics: from Microbial Diversity to metabolic mechanisms. Int. J. Mol. Sci. 25, 593 (2024).
pubmed: 38203764
doi: 10.3390/ijms25010593
Davidov, K. et al. Identification of plastic-associated species in the Mediterranean Sea using DNA metabarcoding with Nanopore MinION. Sci. Rep. 10, 17533 (2020).
pubmed: 33067509
doi: 10.1038/s41598-020-74180-z
Pinto, M., Langer, T. M., Hüffer, T., Hofmann, T. & Herndl, G. J. The composition of bacterial communities associated with plastic biofilms differs between different polymers and stages of biofilm succession. PloS One 14, e0217165 (2019).
pubmed: 31166981
doi: 10.1371/journal.pone.0217165
Wen, B. et al. Community structure and functional diversity of the plastisphere in aquaculture waters: does plastic color matter? Sci. Total Environ. 740, 140082 (2020).
pubmed: 32927571
doi: 10.1016/j.scitotenv.2020.140082
Bhagwat, G. et al. Exploring the composition and functions of plastic microbiome using whole-genome sequencing. Environ. Sci. Technol. 55, 4899–4913 (2021).
pubmed: 33686859
doi: 10.1021/acs.est.0c07952
Jacquin, J. et al. Microbial ecotoxicology of marine plastic debris: a review on colonization and biodegradation by the plastisphere. Front. Microbiol. 10, 865 (2019).
pubmed: 31073297
doi: 10.3389/fmicb.2019.00865
Oberbeckmann, S., Osborn, A. M. & Duhaime, M. B. Microbes on a bottle: substrate, season and geography influence community composition of microbes colonizing marine plastic debris. PLoS One 11, e0159289 (2016).
pubmed: 27487037
doi: 10.1371/journal.pone.0159289
Sérvulo, T. et al. Plastisphere composition in a subtropical estuary: influence of season, incubation time and polymer type on plastic biofouling. Environ. Pollut. 332, 121873 (2023).
pubmed: 37244532
doi: 10.1016/j.envpol.2023.121873
Oberbeckmann, S., Loeder, M. G., Gerdts, G. & Osborn, A. M. Spatial and seasonal variation in diversity and structure of microbial biofilms on marine plastics in northern European waters. FEMS Microbiol. Ecol. 90, 478–492 (2014).
pubmed: 25109340
doi: 10.1111/1574-6941.12409
Zhang, B. et al. Spatial and seasonal variations in biofilm formation on microplastics in coastal waters. Sci. Total Environ. 770, 145303 (2021).
pubmed: 33515883
doi: 10.1016/j.scitotenv.2021.145303
Dong, X. et al. Seasonal biofilm formation on floating microplastics in coastal waters of intensified marinculture area. Mar. Pollut. Bull. 171, 112914 (2021).
pubmed: 34488149
doi: 10.1016/j.marpolbul.2021.112914
Sharma, S., Sharma, V. & Chatterjee, S. Microplastics in the Mediterranean Sea: sources, pollution intensity, sea health, and regulatory policies. Front. Mar. Sci. 8, 634934 (2021).
doi: 10.3389/fmars.2021.634934
van der Hal, N., Ariel, A. & Angel, D. L. Exceptionally high abundances of microplastics in the oligotrophic Israeli Mediterranean coastal waters. Mar. Pollut. Bull. 116, 151–155 (2017).
pubmed: 28063700
doi: 10.1016/j.marpolbul.2016.12.052
Karamitros, T. & Magiorkinis, G. Multiplexed targeted sequencing for oxford nanopore MinION: a detailed library preparation procedure. Next Generation Sequencing Methods Protocols, 43–51 (2018).
Bolyen, E. et al. Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2. Nat. Biotechnol. 37, 852–857 (2019).
pubmed: 31341288
pmcid: 7015180
doi: 10.1038/s41587-019-0209-9
Quast, C. et al. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res. 41, D590–D596 (2012).
pubmed: 23193283
pmcid: 3531112
doi: 10.1093/nar/gks1219
Lu, Y. et al. MicrobiomeAnalyst 2.0: comprehensive statistical, functional and integrative analysis of microbiome data. Nucleic Acids Res. 51, W310–W318 (2023).
pubmed: 37166960
pmcid: 10320150
doi: 10.1093/nar/gkad407
Mallick, H. et al. Multivariable association discovery in population-scale meta-omics studies. PLoS Comput. Biol. 17, e1009442 (2021).
pubmed: 34784344
pmcid: 8714082
doi: 10.1371/journal.pcbi.1009442
Cózar, A. et al. Plastic accumulation in the Mediterranean Sea. PloS One 10, e0121762 (2015).
pubmed: 25831129
doi: 10.1371/journal.pone.0121762
Reich, T. et al. A year in the life of the Eastern Mediterranean: monthly dynamics of phytoplankton and bacterioplankton in an ultra-oligotrophic sea. Deep Sea Res. Part I 182, 103720 (2022).
doi: 10.1016/j.dsr.2022.103720
Mincer, T. J., Zettler, E. R. & Amaral-Zettler, L. A. Biofilms on plastic debris and their influence on marine nutrient cycling, productivity, and hazardous chemical mobility. Hazard. Chem. Assoc. Plast. Mar. Environ. 221–233 (2019).
Wright, R. J., Erni-Cassola, G., Zadjelovic, V., Latva, M. & Christie-Oleza, J. A. Marine plastic debris: a new surface for microbial colonization. Environ. Sci. Technol. 54, 11657–11672 (2020).
pubmed: 32886491
doi: 10.1021/acs.est.0c02305
A Bryant, J. et al. Diversity and activity of communities inhabiting plastic debris in the North Pacific Gyre. MSystems 1, 101128msystems00024–101128msystems00016 (2016).
doi: 10.1128/mSystems.00024-16
Amaral-Zettler, L. A. et al. Diversity and predicted inter-and intra-domain interactions in the Mediterranean plastisphere. Environ. Pollut. 286, 117439 (2021).
pubmed: 34438479
doi: 10.1016/j.envpol.2021.117439
Agostini, L. et al. Deep-sea plastisphere: long-term colonization by plastic-associated bacterial and archaeal communities in the Southwest Atlantic Ocean. Sci. Total Environ. 793, 148335 (2021).
pubmed: 34174607
doi: 10.1016/j.scitotenv.2021.148335
Wang, Q. et al. Colonization characteristics and dynamic transition of archaea communities on polyethylene and polypropylene microplastics in the sediments of mangrove ecosystems. J. Hazard. Mater. 471, 134343 (2024).
pubmed: 38640671
doi: 10.1016/j.jhazmat.2024.134343
Vaksmaa, A. et al. Microbial communities on plastic polymers in the Mediterranean Sea. Front. Microbiol. 12, 673553 (2021).
pubmed: 34220756
doi: 10.3389/fmicb.2021.673553
Nguyen, D., Masasa, M., Ovadia, O. & Guttman, L. Ecological insights into the resilience of marine plastisphere throughout a storm disturbance. Sci. Total Environ. 858, 159775 (2023).
pubmed: 36309286
doi: 10.1016/j.scitotenv.2022.159775
Junaid, M., Siddiqui, J. A., Sadaf, M., Liu, S. & Wang, J. Enrichment and dissemination of bacterial pathogens by microplastics in the aquatic environment. Sci. Total Environ. 830, 154720 (2022).
pubmed: 35337880
doi: 10.1016/j.scitotenv.2022.154720
Haram, L. E. et al. Extent and reproduction of coastal species on plastic debris in the North Pacific Subtropical Gyre. Nat. Ecol. Evol. 7, 687–697 (2023).
pubmed: 37069334
pmcid: 10172146
doi: 10.1038/s41559-023-01997-y
Reisser, J. et al. Millimeter-sized marine plastics: a new pelagic habitat for microorganisms and invertebrates. PloS One 9, e100289 (2014).
pubmed: 24941218
pmcid: 4062529
doi: 10.1371/journal.pone.0100289
Benoiston, A. S. et al. The evolution of diatoms and their biogeochemical functions. Philos. Trans. R. Soc. B Biol. Sci. 372, 20160397 (2017).
doi: 10.1098/rstb.2016.0397
Rousseaux, C. S. & Gregg, W. W. Interannual variation in phytoplankton primary production at a global scale. Remote Sens. 6, 1–19 (2013).
doi: 10.3390/rs6010001
Tréguer, P. et al. Influence of diatom diversity on the ocean biological carbon pump. Nat. Geosci. 11, 27–37 (2018).
doi: 10.1038/s41561-017-0028-x
Baumas, C. & Bizic, M. A focus on different types of organic particles and their significance in the open ocean carbon cycle. Prog. Oceanogr., 103233 (2024).
Kooi, M., Nes, E. H., v., Scheffer, M. & Koelmans, A. A. Ups and downs in the ocean: effects of biofouling on vertical transport of microplastics. Environ. Sci. Technol. 51, 7963–7971 (2017).
pubmed: 28613852
pmcid: 6150669
doi: 10.1021/acs.est.6b04702
Bondoc-Naumovitz, K. G. & Cohn, S. A. Diatom Gliding Motil. 77–109 (2021).
Poulsen, N. & Davutoglu, M. G. & Zackova Suchanova, J. In The Molecular life of Diatoms, 367–393 (Springer, 2022).
Molino, P. J. & Wetherbee, R. The biology of biofouling diatoms and their role in the development of microbial slimes. Biofouling 24, 365–379 (2008).
pubmed: 18604655
doi: 10.1080/08927010802254583
Delacuvellerie, A., Geron, A., Gobert, S. & Wattiez, R. New insights into the functioning and structure of the PE and PP plastispheres from the Mediterranean Sea. Environ. Pollut. 295, 118678 (2022).
pubmed: 34915097
doi: 10.1016/j.envpol.2021.118678