DPP an extracellular matrix molecule induces Wnt5a mediated signaling to promote the differentiation of adult stem cells into odontogenic lineage.
Wnt-5a Protein
/ metabolism
Animals
Cell Differentiation
Odontogenesis
/ genetics
Humans
Mice
Adult Stem Cells
/ metabolism
Dental Pulp
/ cytology
Phosphoproteins
/ metabolism
Wnt Signaling Pathway
Extracellular Matrix Proteins
/ metabolism
Sialoglycoproteins
/ metabolism
Mice, Knockout
beta Catenin
/ metabolism
Odontoblasts
/ metabolism
Cell Lineage
Signal Transduction
Extracellular Matrix
/ metabolism
Frizzled Receptors
/ metabolism
Low Density Lipoprotein Receptor-Related Protein-6
/ metabolism
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
31 10 2024
31 10 2024
Historique:
received:
18
03
2024
accepted:
10
10
2024
medline:
31
10
2024
pubmed:
31
10
2024
entrez:
31
10
2024
Statut:
epublish
Résumé
Dentin phosphophoryn (DPP) an extracellular matrix protein activates Wnt signaling in DPSCs (dental pulp stem cells). Wnt/β catenin signaling is essential for tooth development but the role of DPP-mediated Wnt5a signaling in odontogenesis is not well understood. Wnt5a is typically considered as a non-canonical Wnt ligand that elicits intracellular signals through association with a specific cohort of receptors and co-receptors in a cell and context-dependent manner. In this study, DPP facilitated the interaction of Wnt5a with Frizzled 5 and LRP6 to induce nuclear translocation of β-catenin. β-catenin has several nuclear binding partners that promote the activation of Wnt target genes responsible for odontogenic differentiation. Interestingly, steady increase in the expression of Vangl2 receptor suggest planar cell polarity signaling during odontogenic differentiation. In vitro observations were further strengthened by the low expression levels of Wnt5a and β-catenin in the teeth of DSPP KO mice which exhibit impaired odontoblast differentiation and defective dentin mineralization. Together, this study suggests that the DPP-mediated Wnt5a signaling could be exploited as a therapeutic approach for the differentiation of dental pulp stem cells into functional odontoblasts and dentin regeneration.
Identifiants
pubmed: 39478025
doi: 10.1038/s41598-024-76069-7
pii: 10.1038/s41598-024-76069-7
doi:
Substances chimiques
Wnt-5a Protein
0
Phosphoproteins
0
Extracellular Matrix Proteins
0
dentin sialophosphoprotein
0
Sialoglycoproteins
0
WNT5A protein, human
0
beta Catenin
0
Wnt5a protein, mouse
0
Frizzled Receptors
0
Low Density Lipoprotein Receptor-Related Protein-6
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
26187Subventions
Organisme : the National Institutes of Health
ID : R01 DE028531 and R01 DE031737
Organisme : the National Institutes of Health
ID : R01 DE028531 and R01 DE031737
Organisme : the National Institutes of Health
ID : R01 DE028531 and R01 DE031737
Organisme : the National Institutes of Health
ID : R01 DE028531 and R01 DE031737
Informations de copyright
© 2024. The Author(s).
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