Bioinspired skin-like in vitro model for investigating catheter-related bloodstream infections.
Bacterial adhesion
Catheter-related bloodstream infections (CRBSIs)
Intravenous therapy
Skin-like replicas
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
30 10 2024
30 10 2024
Historique:
received:
17
07
2024
accepted:
15
10
2024
medline:
31
10
2024
pubmed:
31
10
2024
entrez:
31
10
2024
Statut:
epublish
Résumé
Intravenous (IV) catheter-related bloodstream infections (CRBSIs) cause significant risks in healthcare, necessitating advancements in catheter design and materials. This study investigates the effectiveness of Ecoflex, a silicone-based material, in studying CRBSIs through the development of skin-like replicas that mimic human skin properties for use in wearable sensing devices. We characterized the replica's bioinspired surface roughness, wettability, bacterial adhesion, and mechanical properties and validated its performance using in vitro IV simulation. The results demonstrated that the bioinspired model replicates human skin textures with less than 7.5% error for surface roughness ranging from 0.05 μm to 6.3 μm. Wettability tests revealed that the artificial sebum application significantly reduced the static contact angles for deionized water and artificial sweat. Comprehensive mechanical testing revealed material high elasticity and resilience, suitable for dynamic biomedical applications. Bacterial adhesion studies using Staphylococcus epidermidis showed varying adhesion patterns influenced by surface roughness, highlighting the potential for material texture to impact infection risk. In IV therapy simulations, we observed bacterial growth dynamics over the incubation period. Our findings suggest that Ecoflex-based skin-like replicas can serve as a valuable tool for developing and testing new catheters, while the potential for use in other medical innovation devices, including wearable sensing devices, ultimately contributes to improved patient outcomes and infection control strategies.
Identifiants
pubmed: 39478046
doi: 10.1038/s41598-024-76652-y
pii: 10.1038/s41598-024-76652-y
doi:
Substances chimiques
Silicones
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
26167Subventions
Organisme : NIGMS NIH HHS
ID : R21GM150104
Pays : United States
Organisme : NIGMS NIH HHS
ID : R21GM150104
Pays : United States
Organisme : NIGMS NIH HHS
ID : R21GM150104
Pays : United States
Organisme : Department of Defense Office of Naval Research
ID : N00014-23-1-2225
Organisme : National Science Foundation
ID : 1648451
Informations de copyright
© 2024. The Author(s).
Références
Gahlot, R., Nigam, C., Kumar, V., Yadav, G. & Anupurba, S. Catheter-related bloodstream infections. Int. J. Crit. Illn. Inj Sci. 4, 162–167 (2014).
doi: 10.4103/2229-5151.134184
pubmed: 25024944
pmcid: 4093967
Otto, M. Staphylococcus epidermidis – the accidental pathogen. Nat. Rev. Microbiol. 7, 555–567 (2009).
doi: 10.1038/nrmicro2182
pubmed: 19609257
pmcid: 2807625
Pascual, A. Pathogenesis of catheter-related infections: Lessons for new designs. Clin. Microbiol. Infect. 8, 256–264 (2002).
doi: 10.1046/j.1469-0691.2002.00418.x
pubmed: 12047402
Sitges-Serra, A. Strategies for prevention of catheter-related bloodstream infections. Support Care Cancer 7, 391–395 (1999).
doi: 10.1007/s005200050298
pubmed: 10541980
Severn, M. M. & Horswill, A. R. Staphylococcus epidermidis and its dual lifestyle in skin health and infection. Nat. Rev. Microbiol. 21, 97–111 (2023).
doi: 10.1038/s41579-022-00780-3
pubmed: 36042296
Le, K. Y., Park, M. D. & Otto, M. Immune evasion mechanisms of staphylococcus epidermidis biofilm infection. Front. Microbiol. 9, 359 (2018).
doi: 10.3389/fmicb.2018.00359
pubmed: 29541068
pmcid: 5835508
Sharma, S. et al. Microbial Biofilm: A review on formation, infection, antibiotic resistance, control measures, and innovative treatment. Microorganisms 11, 1614 (2023).
doi: 10.3390/microorganisms11061614
pubmed: 37375116
pmcid: 10305407
Cheung, E., Baerlocher, M. O., Asch, M. & Myers, A. Venous access. Can. Fam Physician 55, 494–496 (2009).
pubmed: 19439704
pmcid: 2682308
Hull, E. L., Nichols, M. G. & Foster, T. H. Quantitative broadband near-infrared spectroscopy of tissue-simulating phantoms containing erythrocytes. Phys. Med. Biol. 43, 3381 (1998).
doi: 10.1088/0031-9155/43/11/014
pubmed: 9832022
Spinelli, L. et al. Determination of reference values for optical properties of liquid phantoms based on Intralipid and India ink. Biomed. Opt. Express BOE. 5, 2037–2053 (2014).
doi: 10.1364/BOE.5.002037
pubmed: 25071947
Bir, C. A., Resslar, M. & Stewart, S. Skin penetration surrogate for the evaluation of less lethal kinetic energy munitions. Forensic Sci. Int. 220, 126–129 (2012).
doi: 10.1016/j.forsciint.2012.02.008
pubmed: 22405483
Owda, A. Y. & Casson, A. J. Investigating gelatine based head phantoms for electroencephalography compared to electrical and Ex vivo porcine skin models. IEEE Access. 9, 96722–96738 (2021).
doi: 10.1109/ACCESS.2021.3095220
Große Perdekamp, M. et al. Experimental simulation of reentry shots using a skin-gelatine composite model. Int. J. Legal Med. 123, 419–425 (2009).
doi: 10.1007/s00414-009-0363-6
pubmed: 19636582
Chahat, N., Zhadobov, M., Sauleau, R. & Alekseev, S. I. New method for determining Dielectric properties of skin and phantoms at millimeter waves based on heating kinetics. IEEE Trans. Microwave Theory Tech. 60, 827–832 (2012).
doi: 10.1109/TMTT.2011.2176746
Nebuya, S., Noshiro, M., Brown, B. H., Smallwood, R. H. & Milnes, P. Detection of emboli in vessels using electrical impedance measurements—phantom and electrodes. Physiol. Meas. 26, 111 (2005).
doi: 10.1088/0967-3334/26/2/011
Kirkpatrick, S. J., Wang, R. K., Duncan, D. D., Kulesz-Martin, M. & Lee, K. Imaging the mechanical stiffness of skin lesions by in vivo acousto-optical elastography. Opt. Express OE. 14, 9770–9779 (2006).
doi: 10.1364/OE.14.009770
Manohar, S. et al. SPIE,. Photoacoustic imaging of inhomogeneities embedded in breast tissue phantoms. in Biomedical Optoacoustics IV vol. 4960 64–75 (2003).
Gabriel, C. Tissue equivalent material for hand phantoms. Phys. Med. Biol. 52, 4205 (2007).
doi: 10.1088/0031-9155/52/14/012
pubmed: 17664603
Khan, G. M., Frum, Y., Sarheed, O., Eccleston, G. M. & Meidan, V. M. Assessment of drug permeability distributions in two different model skins. Int. J. Pharm. 303, 81–87 (2005).
doi: 10.1016/j.ijpharm.2005.07.005
pubmed: 16102922
Morales-Hurtado, M., Zeng, X., Gonzalez-Rodriguez, P., Elshof, T., van der Heide, E. & J. E. & A new water absorbable mechanical epidermal skin equivalent: the combination of hydrophobic PDMS and hydrophilic PVA hydrogel. J. Mech. Behav. Biomed. Mater. 46, 305–317 (2015).
doi: 10.1016/j.jmbbm.2015.02.014
pubmed: 25840121
Hwang, H. Y. Piezoelectric particle-reinforced polyurethane for tactile sensing robot skin. Mech. Compos. Mater. 47, 137–144 (2011).
doi: 10.1007/s11029-011-9192-z
Elleuch, K., Elleuch, R. & Zahouani, H. Comparison of elastic and tactile behavior of human skin and elastomeric materials through tribological tests. Polym. Eng. Sci. 46, 1715–1720 (2006).
doi: 10.1002/pen.20637
Moonen, M., et al. A versatile artificial skin platform for sweat sensor development. Lab. Chip. 23, 2268–2275 (2023).
doi: 10.1039/D3LC00109A
pubmed: 37043225
Fitzpatrick, T. B. The validity and practicality of sun-reactive skin types I through VI. Arch. Dermatol. 124, 869–871 (1988).
doi: 10.1001/archderm.1988.01670060015008
pubmed: 3377516
Bloemen, M. C. T., van Gerven, M. S., van der Wal, M. B. A., Verhaegen, P. D. H. M. & Middelkoop, E. An objective device for measuring surface roughness of skin and scars. J. Am. Acad. Dermatol. 64, 706–715 (2011).
doi: 10.1016/j.jaad.2010.03.006
pubmed: 21216493
Hani, A. F. M., Prakasa, E., Nugroho, H., Affandi, A. M. & Hussein, S. H. Sample area for surface roughness determination of skin surfaces. in 4th International Conference on Intelligent and Advanced Systems (ICIAS2012) 1, 328–332 (2012). (2012).
Ohtsuki, R., Sakamaki, T. & Tominaga, S. Analysis of skin surface roughness by visual assessment and surface measurement. Opt. Rev. 20, (2013).
Maiti, R. et al. In vivo measurement of skin surface strain and sub-surface layer deformation induced by natural tissue stretching. J. Mech. Behav. Biomed. Mater. 62, 556–569 (2016).
doi: 10.1016/j.jmbbm.2016.05.035
pubmed: 27310571
Maiti, R. et al. Morphological parametric mapping of 21 skin sites throughout the body using optical coherence tomography. J. Mech. Behav. Biomed. Mater. 102, 103501 (2020).
doi: 10.1016/j.jmbbm.2019.103501
pubmed: 31877514
Suhail, S. et al. Engineered skin tissue equivalents for product evaluation and therapeutic applications. Biotechnol. J. 14, e1900022 (2019).
doi: 10.1002/biot.201900022
pubmed: 30977574
pmcid: 6615970
Bolle, E. C. L. et al. An in vitro reconstructed human skin equivalent model to study the role of skin integration around percutaneous devices against bacterial infection. Front. Microbiol. 11, (2020).
Islam, S. U., Glover, A., MacFarlane, R. J., Mehta, N. & Waseem, M. The anatomy and biomechanics of the elbow. TOORTHJ 14, 95–99 (2020).
doi: 10.2174/1874325002014010095
Ecoflex™ 00–35 FAST Product Information. Smooth-On, Inc. https://www.smooth-on.com/products/ecoflex-00-35/
Ginn, M. E., Noyes, C. M. & Jungermann, E. The contact angle of water on viable human skin. J. Colloid Interface Sci. 26, 146–151 (1968).
doi: 10.1016/0021-9797(68)90306-8
pubmed: 5650915
Zheng, S. et al. Implication of surface properties, bacterial motility, and hydrodynamic conditions on bacterial surface sensing and their initial adhesion. Front. Bioeng. Biotechnol. 9, (2021).
Wang, X., Liu, Y., Cheng, H. & Ouyang, X. Surface wettability for skin-interfaced sensors and devices. Adv. Funct. Mater. 32, 2200260 (2022).
doi: 10.1002/adfm.202200260
pubmed: 36176721
pmcid: 9514151
Kovalev, A. E., Dening, K., Persson, B. N. J. & Gorb, S. N. Surface topography and contact mechanics of dry and wet human skin. Beilstein J. Nanotechnol 5, 1341–1348 (2014).
doi: 10.3762/bjnano.5.147
pubmed: 25247117
pmcid: 4168723
Yoda, I. et al. Effect of surface roughness of biomaterials on Staphylococcus epidermidis adhesion. BMC Microbiol. 14, 234 (2014).
doi: 10.1186/s12866-014-0234-2
pubmed: 25179448
pmcid: 4161769
Dunne, C. P. et al. Anti-microbial coating innovations to prevent infectious diseases (AMiCI): Cost action ca15114. Bioengineered 8, 679–685 (2017).
doi: 10.1080/21655979.2017.1323593
pubmed: 28453429
pmcid: 5736330
Hawas, S., Verderosa, A. D. & Totsika, M. Combination therapies for biofilm inhibition and eradication: A comparative review of Laboratory and Preclinical studies. Front. Cell. Infect. Microbiol. 12, (2022).
Son, M. S. & Taylor, R. K. Growth and maintenance of escherichia coli laboratory strains. Curr. Protoc. 1, e20 (2021).
doi: 10.1002/cpz1.20
pubmed: 33484484
pmcid: 8006063
Oliveira, F., França, Â. & Cerca, N. Staphylococcus epidermidis is largely dependent on iron availability to form biofilms. Int. J. Med. Microbiol. 307, 552–563 (2017).
doi: 10.1016/j.ijmm.2017.08.009
pubmed: 28939440