Polyethyleneimine facilitates the growth and electrophysiological characterization of iPSC-derived motor neurons.

Induced Pluripotent Stem Cells / cytology Motor Neurons / physiology Humans Polyethyleneimine / chemistry Cell Differentiation Electrophysiological Phenomena Cells, Cultured Cell Proliferation
Extracellular matrix Matrigel Multielectrode array Poly-l-ornithine Polyethyleneimine iPSC-derived motor neurons

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
30 10 2024
Historique:
received: 13 07 2024
accepted: 24 10 2024
medline: 31 10 2024
pubmed: 31 10 2024
entrez: 31 10 2024
Statut: epublish

Résumé

Induced pluripotent stem cell (iPSC) technology, in combination with electrophysiological characterization via multielectrode array (MEA), has facilitated the utilization of iPSC-derived motor neurons (iPSC-MNs) as highly valuable models for underpinning pathogenic mechanisms and developing novel therapeutic interventions for motor neuron diseases (MNDs). However, the challenge of MN adherence to the MEA plate and the heterogeneity presented in iPSC-derived cultures raise concerns about the reproducibility of the findings obtained from these cellular models. We discovered that one novel factor modulating the electrophysiological activity of iPSC-MNs is the extracellular matrix (ECM) used in the coating to support in vitro growth, differentiation and maturation of iPSC-MNs. The current study showed that two coating conditions, namely, Poly-L-ornithine/Matrigel (POM) and Polyethyleneimine (PEI) strongly promoted attachment of iPSC-MNs on MEA culture dishes compared to three other coating conditions, and both facilitated the maturation of iPSC-MNs as characterized by the detection of extensive electrophysiological activities from the MEA plates. POM coating accelerated the maturation of the iPSC-MNs for up to 5 weeks, which suits modeling of neurodevelopmental disorders. However, the application of PEI resulted in more even distribution of the MNs on the culture dish and reduced variability of electrophysiological signals from the iPSC-MNs in 7-week cultures, which permitted the detection of enhanced excitability in iPSC-MNs from patients with amyotrophic lateral sclerosis (ALS). This study provides a comprehensive comparison of five coating conditions and offers POM and PEI as favorable coatings for in vitro modeling of neurodevelopmental and neurodegenerative disorders, respectively.

Identifiants

DOI: 10.1038/s41598-024-77710-1 PMID: 39478194
pubmed: 39478194
doi: 10.1038/s41598-024-77710-1
pii: 10.1038/s41598-024-77710-1
doi:

Substances chimiques

Polyethyleneimine 9002-98-6

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

26106

Subventions

Organisme : Science Foundation Ireland
ID : 16/RC/3948
Pays : Ireland
Organisme : Natural Science Foundation of Hebei Province
ID : C2024205026
Organisme : Doctoral Research Initiation Fund Project of Hebei Normal University
ID : L2024B36
Organisme : International Scientific and Technological Cooperation Foundation of Shenzhen
ID : GJHZ20200731095005016
Organisme : Medical-Engineering Interdisciplinary Research Foundation of ShenZhen University
ID : 00000326

Informations de copyright

© 2024. The Author(s).

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Auteurs

Meimei Yang (M)

Key Laboratory of Measurement and Evaluation in Exercise Bioinformation of Hebei Province, School of Physical Education, Hebei Normal University, Shijiazhuang, 050024, China. meimeiyang_2024@163.com.
Regenerative Medicine Institute, School of Medicine, University of Galway, Galway, H91 W2TY, Ireland. meimeiyang_2024@163.com.
FutureNeuro SFI Research Centre for Chronic and Rare Neurological Diseases and Department of Physiology and Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland. meimeiyang_2024@163.com.

Daofeng You (D)

Emergency Department, The First Hospital of Hebei Medical University, No. 89, Donggang Road, Shijiazhuang, China.

Gang Liu (G)

Department of Cardiology, Hebei Key Laboratory of Cardiac Injury Repair Mechanism Study; Hebei Key Laboratory of Heart and Metabolism, Hebei Engineering Research Center of Intelligent Medical Clinical Application, Hebei International Joint Research Center for Structural Heart Disease, The First Hospital of Hebei Medical University, Shijiazhuang, China.

Yin Lu (Y)

College of Pharmacy, Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine (TCM) Prevention and Treatment of Tumor, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210023, China.

Guangming Yang (G)

College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, Jiangsu, China.
Confucius Institute of Chinese and Regenerative Medicine, University of Galway, Galway, H91 W2TY, Ireland.

Timothy O'Brien (T)

Regenerative Medicine Institute, School of Medicine, University of Galway, Galway, H91 W2TY, Ireland.
Confucius Institute of Chinese and Regenerative Medicine, University of Galway, Galway, H91 W2TY, Ireland.

David C Henshall (DC)

FutureNeuro SFI Research Centre for Chronic and Rare Neurological Diseases and Department of Physiology and Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland.
Department of Physiology and Medical Physics, RCSI University of Medicine & Health Sciences, Dublin, D02 YN77, Ireland.

Orla Hardiman (O)

FutureNeuro SFI Research Centre for Chronic and Rare Neurological Diseases and Department of Physiology and Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland.
Academic Unit of Neurology, Trinity Biomedical Sciences Institute, Trinity College Dublin, 152-160 Pearse Street, Dublin 2, Ireland.

Li Cai (L)

Department of Ophthalmology, Shenzhen University General Hospital, Xueyuan Road 1098, Shenzhen, 518000, China. caili@szu.edu.cn.

Min Liu (M)

Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, China. minliu3649@163.com.

Sanbing Shen (S)

Regenerative Medicine Institute, School of Medicine, University of Galway, Galway, H91 W2TY, Ireland. sanbing.shen@universityofgalway.ie.
FutureNeuro SFI Research Centre for Chronic and Rare Neurological Diseases and Department of Physiology and Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland. sanbing.shen@universityofgalway.ie.
Confucius Institute of Chinese and Regenerative Medicine, University of Galway, Galway, H91 W2TY, Ireland. sanbing.shen@universityofgalway.ie.

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches pluripotentes induites › Polyethyleneimine facilitates the growth and...
questionsmedicales.fr › Cellules › Neurones › Neurones efférents › Motoneurones › Polyethyleneimine facilitates the growth and...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Polyethyleneimine facilitates the growth and...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Matières plastiques › Polyéthylènes › Polyéthylèneimine › Polyethyleneimine facilitates the growth and...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Polyethyleneimine facilitates the growth and...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes électrophysiologiques › Polyethyleneimine facilitates the growth and...
questionsmedicales.fr › Cellules › Cellules cultivées › Polyethyleneimine facilitates the growth and...
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Croissance › Processus de croissance cellulaire › Prolifération cellulaire › Polyethyleneimine facilitates the growth and...