Physiological phenotyping of transpiration response to vapour pressure deficit in wheat.
Drought stress
High-throughput phenotyping
Transpiration restriction
Vapour pressure deficit
Water use efficiency
Wheat
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
30 Oct 2024
30 Oct 2024
Historique:
received:
24
04
2024
accepted:
11
10
2024
medline:
31
10
2024
pubmed:
31
10
2024
entrez:
31
10
2024
Statut:
epublish
Résumé
Precision phenotyping of short-term transpiration response to environmental conditions and transpiration patterns throughout wheat development enables a better understanding of specific trait compositions that lead to improved transpiration efficiency. Transpiration and related traits were evaluated in a set of 79 winter wheat lines using the custom-built "DroughtSpotter XXL" facility. The 120 l plant growth containers implemented in this phenotyping platform enable gravimetric quantification of water use in real-time under semi-controlled, yet field-like conditions across the entire crop life cycle. The resulting high-resolution data enabled identification of significant developmental stage-specific variation for genotype rankings in transpiration efficiency. In addition, for all examined genotypes we identified the genotype-specific breakpoint in transpiration in response to increasing vapour pressure deficit, with breakpoints ranging between 2.75 and 4.1 kPa. Continuous monitoring of transpiration efficiency and diurnal transpiration patterns enables identification of hidden, heritable genotypic variation for transpiration traits relevant for wheat under drought stress. Since the unique experimental setup mimics field-like growth conditions, the results of this study have good transferability to field conditions.
Sections du résumé
BACKGROUND
BACKGROUND
Precision phenotyping of short-term transpiration response to environmental conditions and transpiration patterns throughout wheat development enables a better understanding of specific trait compositions that lead to improved transpiration efficiency. Transpiration and related traits were evaluated in a set of 79 winter wheat lines using the custom-built "DroughtSpotter XXL" facility. The 120 l plant growth containers implemented in this phenotyping platform enable gravimetric quantification of water use in real-time under semi-controlled, yet field-like conditions across the entire crop life cycle.
RESULTS
RESULTS
The resulting high-resolution data enabled identification of significant developmental stage-specific variation for genotype rankings in transpiration efficiency. In addition, for all examined genotypes we identified the genotype-specific breakpoint in transpiration in response to increasing vapour pressure deficit, with breakpoints ranging between 2.75 and 4.1 kPa.
CONCLUSION
CONCLUSIONS
Continuous monitoring of transpiration efficiency and diurnal transpiration patterns enables identification of hidden, heritable genotypic variation for transpiration traits relevant for wheat under drought stress. Since the unique experimental setup mimics field-like growth conditions, the results of this study have good transferability to field conditions.
Identifiants
pubmed: 39478466
doi: 10.1186/s12870-024-05692-3
pii: 10.1186/s12870-024-05692-3
doi:
Substances chimiques
Water
059QF0KO0R
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1032Informations de copyright
© 2024. The Author(s).
Références
IPPC. Climate Change 2022: Mitigation of Climate Change. Contribution of Working Group III to the Sixth Assessment Report of the Intergovernmental Panel on Climate Change. 2022.
Lobell DB, Hammer GL, McLean G, Messina C, Roberts MJ, Schlenker W. The critical role of extreme heat for maize production in the United States. Nat Clim Change. 2013;3(5):497–501.
Snowdon RJ, Wittkop B, Chen TW, Stahl A. Crop adaptation to climate change as a consequence of long-term breeding. Theor Appl Genet. 2021;134(6):1613–23.
pubmed: 33221941
doi: 10.1007/s00122-020-03729-3
Tardieu F. Any trait or trait-related allele can confer drought tolerance: just design the right drought scenario. J Exp Bot. 2012;63(1):25–31.
pubmed: 21963615
doi: 10.1093/jxb/err269
Vadez V, Kholova J, Medina S, Kakkera A, Anderberg H. Transpiration efficiency: new insights into an old story. J Exp Bot. 2014;65(21):6141–53.
pubmed: 24600020
doi: 10.1093/jxb/eru040
Kirkegaard JA, Hunt JR. Increasing productivity by matching farming system management and genotype in water-limited environments. J Exp Bot. 2010;61(15):4129–43.
pubmed: 20709725
doi: 10.1093/jxb/erq245
Passioura J. Grain yield, harvest index, and water use of wheat. J Aust Inst Agric Sci. 1977;43:117–20.
Vadez V. Root hydraulics: the forgotten side of roots in drought adaptation. Field Crops Res. 2014;165:15–24.
doi: 10.1016/j.fcr.2014.03.017
Manschadi AM, Christopher J, deVoil P, Hammer GL, Manschadi AM, Christopher J, et al. The role of root architectural traits in adaptation of wheat to water-limited environments. Funct Plant Biol. 2006;33(9):823–37.
Lilley JM, Kirkegaard JA. Benefits of increased soil exploration by wheat roots. Field Crops Res. 2011;122(2):118–30.
doi: 10.1016/j.fcr.2011.03.010
Schoppach R, Wauthelet D, Jeanguenin L, Sadok W. Conservative water use under high evaporative demand associated with smaller root metaxylem and limited trans-membrane water transport in wheat. Funct Plant Biol. 2014;41(3):257.
pubmed: 32480986
doi: 10.1071/FP13211
Maurel C, Boursiac Y, Luu DT, Santoni V, Shahzad Z, Verdoucq L. Aquaporins in plants. Physiol Rev. 2015;95(4):1321–58.
pubmed: 26336033
doi: 10.1152/physrev.00008.2015
Patel J, Mishra A. Plant aquaporins alleviate drought tolerance in plants by modulating cellular biochemistry, root-architecture, and photosynthesis. Physiol Plant. 2021;172(2):1030–44.
pubmed: 33421148
doi: 10.1111/ppl.13324
Calvo-Polanco M, Ribeyre Z, Dauzat M, Reyt G, Hidalgo-Shrestha C, Diehl P, et al. Physiological roles of Casparian strips and suberin in the transport of water and solutes. New Phytol. 2021;232(6):2295–307.
pubmed: 34617285
pmcid: 9298204
doi: 10.1111/nph.17765
Richards R, Passioura J. A breeding program to reduce the diameter of the major xylem vessel in the seminal roots of wheat and its effect on grain yield in rain-fed environments. Aust J Agric Res. 1989;40(5):943.
doi: 10.1071/AR9890943
Kholová J, Hash CT, Kumar PL, Yadav RS, Kočová M, Vadez V. Terminal drought-tolerant pearl millet [Pennisetum glaucum (L.) R. Br.] have high leaf ABA and limit transpiration at high vapour pressure deficit. J Exp Bot. 2010;61(5):1431–40.
Vadez V, Kholová J, Yadav RS, Hash CT. Small temporal differences in water uptake among varieties of pearl millet (Pennisetum glaucum (L.) R. Br.) are critical for grain yield under terminal drought. Plant Soil. 2013;371(1):447–62.
Sinclair TR. Effective water use required for improving crop growth rather than transpiration efficiency. Front Plant Sci. 2018;9:1442. https://doi.org/10.3389/fpls.2018.01442
Schoppach R, Sadok W. Differential sensitivities of transpiration to evaporative demand and soil water deficit among wheat elite cultivars indicate different strategies for drought tolerance. Environ Exp Bot. 2012;84:1–10.
doi: 10.1016/j.envexpbot.2012.04.016
Vadez V, Kholova J, Zaman-Allah M, Belko N. Water: the most important ‘molecular’ component of water stress tolerance research. Funct Plant Biol. 2013;40(12):1310–22.
pubmed: 32481197
doi: 10.1071/FP13149
Sinclair TR, Devi J, Shekoofa A, Choudhary S, Sadok W, Vadez V, et al. Limited-transpiration response to high vapor pressure deficit in crop species. Plant Sci. 2017;260:109–18.
pubmed: 28554468
doi: 10.1016/j.plantsci.2017.04.007
Sinclair TR, Zwieniecki MA, Holbrook NM. Low leaf hydraulic conductance associated with drought tolerance in soybean. Physiol Plant. 2008;132(4):446–51.
pubmed: 18333998
doi: 10.1111/j.1399-3054.2007.01028.x
Sadok W, Sinclair TR. Transpiration response of ‘slow-wilting’ and commercial soybean (Glycine max (L.) Merr.) genotypes to three aquaporin inhibitors. J Exp Bot. 2010;61(3):821–9.
pubmed: 19969533
doi: 10.1093/jxb/erp350
Sadok W, Sinclair TR. Genetic variability of transpiration response of soybean [Glycine max (L.) Merr.] shoots to leaf hydraulic conductance inhibitor AgNO
Choudhary S, Sinclair T, Messina C, Cooper M. Hydraulic conductance of maize hybrids differing in transpiration response to vapor pressure deficit. Crop Sci. 2014;54:1147.
doi: 10.2135/cropsci2013.05.0303
Collins B, Chapman S, Hammer G, Chenu K. Limiting transpiration rate in high evaporative demand conditions to improve Australian wheat productivity. in silico Plants. 2021;3(1):diab006. https://doi.org/10.1093/insilicoplants/diab006
Schoppach R, Fleury D, Sinclair TR, Sadok W. Transpiration sensitivity to evaporative demand across 120 years of breeding of Australian wheat cultivars. J Agron Crop Sci. 2017;203(3):219–26.
doi: 10.1111/jac.12193
Zaman-Allah M, Jenkinson DM, Vadez V. Chickpea genotypes contrasting for seed yield under terminal drought stress in the field differ for traits related to the control of water use. Funct Plant Biol. 2011;38(4):270–81.
pubmed: 32480883
doi: 10.1071/FP10244
Kholová J, Murugesan T, Kaliamoorthy S, Malayee S, Baddam R, Hammer GL, et al. Modelling the effect of plant water use traits on yield and stay-green expression in sorghum. Funct Plant Biol. 2014;41(11):1019–34.
pubmed: 32481055
doi: 10.1071/FP13355
Messina CD, Sinclair TR, Hammer GL, Curan D, Thompson J, Oler Z, et al. Limited-transpiration trait may increase maize drought tolerance in the US corn belt. Agron J. 2015;107(6):1978–86.
doi: 10.2134/agronj15.0016
Medina S, Vicente R, Nieto-Taladriz MT, Aparicio N, Chairi F, Vergara-Diaz O, et al. The plant-transpiration response to vapor pressure deficit (VPD) in durum wheat is associated with differential yield performance and specific expression of genes involved in primary metabolism and water transport. Front Plant Sci. 2019;9:1994.
pubmed: 30697225
pmcid: 6341309
doi: 10.3389/fpls.2018.01994
Gosa SC, Lupo Y, Moshelion M. Quantitative and comparative analysis of whole-plant performance for functional physiological traits phenotyping: New tools to support pre-breeding and plant stress physiology studies. Plant Sci. 2019;282:49–59.
pubmed: 31003611
doi: 10.1016/j.plantsci.2018.05.008
Negin B, Moshelion M. The advantages of functional phenotyping in pre-field screening for drought-tolerant crops. Funct Plant Biol. 2017;44(1):107–18.
doi: 10.1071/FP16156
Sadras VO. Effective phenotyping applications require matching trait and platform and more attention to theory. Front Plant Sci. 2019;10:1339. https://doi.org/10.3389/fpls.2019.01339
Langstroff A, Heuermann MC, Stahl A, Junker A. Opportunities and limits of controlled-environment plant phenotyping for climate response traits. Theor Appl Genet. 2022;135(1):1–16.
pubmed: 34302493
doi: 10.1007/s00122-021-03892-1
Stahl A, Wittkop B, Snowdon RJ. High-resolution digital phenotyping of water uptake and transpiration efficiency. Trends Plant Sci. 2020;25(5):429–33.
pubmed: 32304656
doi: 10.1016/j.tplants.2020.02.001
Poorter H, Bühler J, van Dusschoten D, Climent J, Postma JA. Pot size matters: a meta-analysis of the effects of rooting volume on plant growth. Funct Plant Biol. 2012;39(11):839.
pubmed: 32480834
doi: 10.1071/FP12049
Poorter H, Fiorani F, Pieruschka R, Wojciechowski T, van der Putten WH, Kleyer M, et al. Pampered inside, pestered outside? Differences and similarities between plants growing in controlled conditions and in the field. New Phytol. 2016;212(4):838–55.
pubmed: 27783423
doi: 10.1111/nph.14243
Hohmann M, Stahl A, Rudloff J, Wittkop B, Snowdon RJ. Not a load of rubbish: simulated field trials in large-scale containers. Plant Cell Environ. 2016;39(9):2064–73.
pubmed: 27144906
doi: 10.1111/pce.12737
Vadez V, Kholová J, Hummel G, Zhokhavets U, Gupta SK, Hash CT. LeasyScan: a novel concept combining 3D imaging and lysimetry for high-throughput phenotyping of traits controlling plant water budget. J Exp Bot. 2015;66(18):5581.
pubmed: 26034130
pmcid: 4585418
doi: 10.1093/jxb/erv251
Knoch D, Abbadi A, Grandke F, Meyer RC, Samans B, Werner CR, et al. Strong temporal dynamics of QTL action on plant growth progression revealed through high-throughput phenotyping in canola. Plant Biotechnol J. 2020;18(1):68–82.
pubmed: 31125482
doi: 10.1111/pbi.13171
Ryan AC, Dodd IC, Rothwell SA, Jones R, Tardieu F, Draye X, et al. Gravimetric phenotyping of whole plant transpiration responses to atmospheric vapour pressure deficit identifies genotypic variation in water use efficiency. Plant Sci. 2016;251:101–9.
pubmed: 27593468
doi: 10.1016/j.plantsci.2016.05.018
Luo T, Hu L, Zhang H, Passioura J, Luo T, Hu L, et al. Genotypic variation of conservative and profligate water use in the vegetative and reproductive stages of canola (Brassica napus L). Funct Plant Biol. 2022;49(3):231–44.
pubmed: 34991784
doi: 10.1071/FP21239
Vukasovic S, Eckert A, Moritz A, Borsch C, Rudloff S, Snowdon R, et al. Effect of a QTL on wheat chromosome 5B associated with enhanced root dry mass on transpiration and nitrogen uptake under contrasting drought scenarios in wheat. BMC Plant Biol. 2024;24.
Kjaer KH, Ottosen CO. 3D laser triangulation for plant phenotyping in challenging environments. Sens. 2015;15(6):13533–47.
doi: 10.3390/s150613533
Sadok W, Sinclair TR. Genetic variability of transpiration response to vapor pressure deficit among soybean cultivars. Crop Sci. 2009;49(3):955–60.
doi: 10.2135/cropsci2008.09.0560
Piepho HP, Möhring J. Computing heritability and selection response from unbalanced plant breeding trials. Genet. 2007;177(3):1881–8.
doi: 10.1534/genetics.107.074229
Muggeo VMR. Interval estimation for the breakpoint in segmented regression: a smoothed score-based approach. Aust N Z J Stat. 2017;59(3):311–22.
doi: 10.1111/anzs.12200
Kuznetsova A, Brockhoff PB, Christensen RHB. lmerTest Package: tests in Linear mixed effects models. J Stat Softw. 2017;82:1–26.
doi: 10.18637/jss.v082.i13
Lenth RV. emmeans: estimated marginal means, aka least-squares means. 2021. https://CRAN.R-project.org/package=emmeans
Hothorn T, Bretz F, Westfall P. Simultaneous inference in general parametric models. Biom J Biom Z. 2008;50(3):346–63.
doi: 10.1002/bimj.200810425
Poorter H, Fiorani F, Stitt M, Schurr U, Finck A, Gibon Y, et al. The art of growing plants for experimental purposes: a practical guide for the plant biologist. Funct Plant Biol. 2012;39(11):821.
pubmed: 32480833
doi: 10.1071/FP12028
Kar S, Tanaka R, Korbu LB, Kholová J, Iwata H, Durbha SS, et al. Automated discretization of ‘transpiration restriction to increasing VPD’ features from outdoors high-throughput phenotyping data. Plant Methods. 2020;16(1):140.
pubmed: 33072176
pmcid: 7565372
doi: 10.1186/s13007-020-00680-8
Blum A. Effective use of water (EUW) and not water-use efficiency (WUE) is the target of crop yield improvement under drought stress. Field Crops Res. 2009;112(2):119–23.
doi: 10.1016/j.fcr.2009.03.009
Fletcher AL, Sinclair TR, Allen LH. Transpiration responses to vapor pressure deficit in well watered ‘slow-wilting’ and commercial soybean. Environ Exp Bot. 2007;61(2):145–51.
doi: 10.1016/j.envexpbot.2007.05.004
Gholipoor M, Choudhary S, Sinclair TR, Messina CD, Cooper M. Transpiration response of maize hybrids to atmospheric vapour pressure deficit. J Agron Crop Sci. 2013;199(3):155–60.
doi: 10.1111/jac.12010
Shekoofa A, Balota M, Sinclair TR. Limited-transpiration trait evaluated in growth chamber and field for sorghum genotypes. Environ Exp Bot. 2014;99:175–9.
doi: 10.1016/j.envexpbot.2013.11.018
Devi MJ, Sinclair TR, Vadez V. Genotypic variation in peanut for transpiration response to vapor pressure deficit. Crop Sci. 2010;50(1):191–6.
doi: 10.2135/cropsci2009.04.0220
Schoppach R, Sadok W. Transpiration sensitivities to evaporative demand and leaf areas vary with night and day warming regimes among wheat genotypes. Funct Plant Biol. 2013;40(7):708–18.
doi: 10.1071/FP13028
Zhang HY, Liu MR, Feng ZH, Song L, Li X, Liu WD, et al. Estimations of water use efficiency in winter wheat based on multi-angle remote sensing. Front Plant Sci. 2021;12:614417.
doi: 10.3389/fpls.2021.614417
pubmed: 35399196
pmcid: 8759039