Scalable production of uniform and mature organoids in a 3D geometrically-engineered permeable membrane.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
31 Oct 2024
31 Oct 2024
Historique:
received:
18
12
2023
accepted:
30
09
2024
medline:
1
11
2024
pubmed:
1
11
2024
entrez:
1
11
2024
Statut:
epublish
Résumé
The application of organoids has been limited by the lack of methods for producing uniformly mature organoids at scale. This study introduces an organoid culture platform, called UniMat, which addresses the challenges of uniformity and maturity simultaneously. UniMat is designed to not only ensure consistent organoid growth but also facilitate an unrestricted supply of soluble factors by a 3D geometrically-engineered, permeable membrane-based platform. Using UniMat, we demonstrate the scalable generation of kidney organoids with enhanced uniformity in both structure and function compared to conventional methods. Notably, kidney organoids within UniMat show improved maturation, showing increased expression of nephron transcripts, more in vivo-like cell-type balance, enhanced vascularization, and better long-term stability. Moreover, UniMat's design offers a more standardized organoid model for disease modeling and drug testing, as demonstrated by polycystic-kidney disease and acute kidney injury modeling. In essence, UniMat presents a valuable platform for organoid technology, with potential applications in organ development, disease modeling, and drug screening.
Identifiants
pubmed: 39482314
doi: 10.1038/s41467-024-53073-z
pii: 10.1038/s41467-024-53073-z
doi:
Substances chimiques
Membranes, Artificial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
9420Subventions
Organisme : National Research Foundation of Korea (NRF)
ID : NRF-2019M3A9H1103769
Organisme : National Research Foundation of Korea (NRF)
ID : RS-2023-00208702
Organisme : National Research Foundation of Korea (NRF)
ID : NRF-2022M3A91015716
Organisme : National Research Foundation of Korea (NRF)
ID : NRF-2020R1C1C1014753
Organisme : Ministry of Trade, Industry and Energy (Ministry of Trade, Industry and Energy, Korea)
ID : 20012378
Informations de copyright
© 2024. The Author(s).
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