Impact of red and blue monochromatic light on the visual system and dopamine pathways in juvenile zebrafish.
Blue light
Dopamine pathway
Monochromatic light
Red light
Visual development
Zebrafish
Journal
BMC ophthalmology
ISSN: 1471-2415
Titre abrégé: BMC Ophthalmol
Pays: England
ID NLM: 100967802
Informations de publication
Date de publication:
31 Oct 2024
31 Oct 2024
Historique:
received:
14
05
2024
accepted:
22
10
2024
medline:
1
11
2024
pubmed:
1
11
2024
entrez:
1
11
2024
Statut:
epublish
Résumé
The development of the zebrafish visual system is significantly influenced by exposure to monochromatic light, yet investigations into its effects during juvenile stages are lacking. This study evaluated the impacts of varying intensities and durations of red and blue monochromatic light on the visual system and dopamine pathways in juvenile zebrafish. Juvenile zebrafish were exposed to red (650 nm) and blue (440 nm, 460 nm) monochromatic lights over four days at intensities ranging from 500 to 10,000 lx, for durations of 6, 10, and 14 h daily. A control group was maintained under standard laboratory conditions. Post-exposure assessments included the optokinetic response (OKR), retinal structural analysis, ocular dopamine levels, and the expression of genes related to dopamine pathways (Th, Dat, and Mao). (1) OKR enhancement was observed with increased 440 nm light intensity, while 460 nm and 650 nm light exposures showed initial improvements followed by declines at higher intensities. (2) Retinal thinning in the outer nuclear layer was observed under the most intense (10,000 lx for 14 h) light conditions in the 440 nm and 650 nm groups, while the 460 nm group remained unaffected. (3) Dopamine levels increased with higher intensities in the 440 nm group, whereas the 460 nm group exhibited initial increases followed by decreases. The 650 nm group displayed similar trends but were statistically insignificant compared to the control group. (4) Th expression increased with light intensity in the 440 nm group. Dat showed a rising and then declining pattern, and Mao expression significantly decreased. The 460 nm group exhibited similar patterns for Th and Dat to the behavioral observations, but an inverse pattern for Mao. The 650 nm group presented significant fluctuations in Th and Dat expressions, with pronounced variations in Mao. Specific red and blue monochromatic light conditions promote visual system development in juvenile zebrafish. However, exceeding these optimal conditions may impair visual function, highlighting the critical role of dopamine pathway in modulating light-induced effects on the visual system.
Sections du résumé
BACKGROUND
BACKGROUND
The development of the zebrafish visual system is significantly influenced by exposure to monochromatic light, yet investigations into its effects during juvenile stages are lacking. This study evaluated the impacts of varying intensities and durations of red and blue monochromatic light on the visual system and dopamine pathways in juvenile zebrafish.
METHODS
METHODS
Juvenile zebrafish were exposed to red (650 nm) and blue (440 nm, 460 nm) monochromatic lights over four days at intensities ranging from 500 to 10,000 lx, for durations of 6, 10, and 14 h daily. A control group was maintained under standard laboratory conditions. Post-exposure assessments included the optokinetic response (OKR), retinal structural analysis, ocular dopamine levels, and the expression of genes related to dopamine pathways (Th, Dat, and Mao).
RESULTS
RESULTS
(1) OKR enhancement was observed with increased 440 nm light intensity, while 460 nm and 650 nm light exposures showed initial improvements followed by declines at higher intensities. (2) Retinal thinning in the outer nuclear layer was observed under the most intense (10,000 lx for 14 h) light conditions in the 440 nm and 650 nm groups, while the 460 nm group remained unaffected. (3) Dopamine levels increased with higher intensities in the 440 nm group, whereas the 460 nm group exhibited initial increases followed by decreases. The 650 nm group displayed similar trends but were statistically insignificant compared to the control group. (4) Th expression increased with light intensity in the 440 nm group. Dat showed a rising and then declining pattern, and Mao expression significantly decreased. The 460 nm group exhibited similar patterns for Th and Dat to the behavioral observations, but an inverse pattern for Mao. The 650 nm group presented significant fluctuations in Th and Dat expressions, with pronounced variations in Mao.
CONCLUSIONS
CONCLUSIONS
Specific red and blue monochromatic light conditions promote visual system development in juvenile zebrafish. However, exceeding these optimal conditions may impair visual function, highlighting the critical role of dopamine pathway in modulating light-induced effects on the visual system.
Identifiants
pubmed: 39482637
doi: 10.1186/s12886-024-03742-w
pii: 10.1186/s12886-024-03742-w
doi:
Substances chimiques
Dopamine
VTD58H1Z2X
Dopamine Plasma Membrane Transport Proteins
0
Monoamine Oxidase
EC 1.4.3.4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
475Subventions
Organisme : National Natural Science Foundation of China
ID : 81670900
Informations de copyright
© 2024. The Author(s).
Références
Dong J, Zhu Z, Xu H, He M. Myopia control effect of repeated low-level red-light therapy in Chinese children: a randomized, double-blind, controlled clinical trial. Ophthalmology. 2023;130(2):198–204.
doi: 10.1016/j.ophtha.2022.08.024
pubmed: 36049646
Burton MJ, Ramke J, Marques AP, Bourne RR, Congdon N, Jones I, Tong BAA, Arunga S, Bachani D, Bascaran C. The lancet global health commission on global eye health: vision beyond 2020. Lancet Glob Health. 2021;9(4):e489–551.
doi: 10.1016/S2214-109X(20)30488-5
pubmed: 33607016
pmcid: 7966694
Medina A. The cause of myopia development and progression: theory, evidence, and treatment. Surv Ophthalmol. 2022;67(2):488–509.
doi: 10.1016/j.survophthal.2021.06.005
pubmed: 34181975
Jiang D, Lin H, Li C, Liu L, Xiao H, Lin Y, Huang X, Chen Y. Longitudinal association between myopia and parental myopia and outdoor time among students in Wenzhou: a 2.5-year longitudinal cohort study. BMC Ophthalmol. 2021;21:1–8.
doi: 10.1186/s12886-020-01763-9
Grzybowski A, Kanclerz P, Tsubota K, Lanca C, Saw SM. A review on the epidemiology of myopia in school children worldwide. BMC Ophthalmol. 2020;20:1–11.
doi: 10.1186/s12886-019-1220-0
Clark R, Kneepkens SC, Plotnikov D, Shah RL, Huang Y, Tideman JWL, Klaver CC, Atan D, Williams C, Guggenheim JAJIO, et al. Time spent outdoors partly accounts for the effect of education on myopia. Invest Ophthalmol Vis Sci. 2023;64(14):38–38.
doi: 10.1167/iovs.64.14.38
pubmed: 38010695
pmcid: 10683767
Tideman W, Kneepkens S, Polling JR, Klaver CC. Time spent outdoors is important to prevent onset and reduce progression of school myopia. Invest Ophthalmol Vis Sci. 2023;64(8):1962–1962.
Dhakal R, Shah R, Huntjens B, Verkicharla PK, Lawrenson JG. Time spent outdoors as an intervention for myopia prevention and control in children: an overview of systematic reviews. Ophthalmic Physiol Opt. 2022;42(3):545–58.
doi: 10.1111/opo.12945
pubmed: 35072278
pmcid: 9305934
Chou HD, Yao TC, Huang YS, Huang CY, Yang ML, Sun MH, Chen HC, Liu CH, Chu SM, Hsu JF. Myopia in school-aged children with preterm birth: the roles of time spent outdoors and serum vitamin D. Br J Ophthalmol. 2021;105(4):468–72.
doi: 10.1136/bjophthalmol-2019-315663
pubmed: 32561534
Chen S, Zhi Z, Ruan Q, Liu Q, Li F, Wan F, Reinach PS, Chen J, Qu J, Zhou X. Bright light suppresses form-deprivation myopia development with activation of dopamine D1 receptor signaling in the ON pathway in retina. Invest Ophthalmol Vis Sci. 2017;58(4):2306–16.
doi: 10.1167/iovs.16-20402
pubmed: 28431434
Yang J, Ouyang X, Fu H, Hou X, Liu Y, Xie Y, Yu H, Wang G. Advances in biomedical study of the myopia-related signaling pathways and mechanisms. Biomed Pharmacother. 2022;145:112472.
doi: 10.1016/j.biopha.2021.112472
pubmed: 34861634
Brandies R, Yehuda S. The possible role of retinal dopaminergic system in visual performance. Neurosci Biobehav Rev. 2008;32(4):611–56.
doi: 10.1016/j.neubiorev.2007.09.004
pubmed: 18061262
Li L, Dowling JE. Effects of dopamine depletion on visual sensitivity of zebrafish. J Neurosci. 2000;20(5):1893–903.
doi: 10.1523/JNEUROSCI.20-05-01893.2000
pubmed: 10684890
pmcid: 6772905
Feldkaemper M, Schaeffel F. An updated view on the role of dopamine in myopia. Exp Eye Res. 2013;114:106–19.
doi: 10.1016/j.exer.2013.02.007
pubmed: 23434455
Lan W, Yang Z, Feldkaemper M, Schaeffel F. Changes in dopamine and ZENK during suppression of myopia in chicks by intense illuminance. Exp Eye Res. 2016;145:118–24.
doi: 10.1016/j.exer.2015.11.018
pubmed: 26657138
Jiang Y, Zhang S, Zhang X, Li N, Zhang Q, Guo X, Chi X, Tong M. Peptidomic analysis of zebrafish embryos exposed to polychlorinated biphenyls and their impact on eye development. Ecotoxicol Environ Saf. 2019;175:164–72.
doi: 10.1016/j.ecoenv.2019.03.015
pubmed: 30897415
Zhang X, Hong Q, Yang L, Zhang M, Guo X, Chi X, Tong M. PCB1254 exposure contributes to the abnormalities of optomotor responses and influence of the photoreceptor cell development in zebrafish larvae. Ecotoxicol Environ Saf. 2015;118:133–8.
doi: 10.1016/j.ecoenv.2015.04.026
pubmed: 25938693
Wei N, Zhang X, Hong Q, Jiang Y, Zhang Q, Guo X, Chi X, Tong M, Liu Q. The sonic hedgehog signaling pathway is suppressed following PCB1254 exposure during retinal development. Environ Toxicol. 2019;34(3):340–7.
doi: 10.1002/tox.22689
pubmed: 30578594
Li Z, Zhang L, Leung YF. Use of the zebrafish model to study refractive error. Expert Rev Ophthalmol. 2013;8(1):1–3.
doi: 10.1586/eop.12.78
Li F, Lin J, Liu X, Li W, Ding Y, Zhang Y, Zhou S, Guo N, Li Q. Characterization of the locomotor activities of zebrafish larvae under the influence of various neuroactive drugs. Ann Transl Med. 2018;6(10):173.
doi: 10.21037/atm.2018.04.25
pubmed: 29951495
Lin W, Huang Z, Zhang W, Ren Y. Investigating the neurotoxicity of environmental pollutants using zebrafish as a model organism: a review and recommendations for future work. Neurotoxicology. 2023;94:235–44.
doi: 10.1016/j.neuro.2022.12.009
pubmed: 36581008
LeFauve MK, Rowe CJ, Crowley-Perry M, Wiegand JL, Shapiro AG, Connaughton VP. Using a variant of the optomotor response as a visual defect detection assay in zebrafish. J Microbiol Methods. 2021;8(1):e144.
Cameron DJ, Rassamdana F, Tam P, Dang K, Yanez C, Ghaemmaghami S, Dehkordi MI. The optokinetic response as a quantitative measure of visual acuity in zebrafish. J Vis Exp. 2013;(80):e50832.
Dehmelt FA, von Daranyi A, Leyden C, Arrenberg AB. Evoking and tracking zebrafish eye movement in multiple larvae with ZebEyeTrack. Nat Protoc. 2018;13(7):1539–68.
doi: 10.1038/s41596-018-0002-0
pubmed: 29988103
Emran F, Rihel J, Dowling JE. A behavioral assay to measure responsiveness of zebrafish to changes in light intensities. J Vis Exp. 2008;(20):e923.
Landis E, Chrenek M, Chakraborty R, Strickland R, Bergen M, Yang V, Iuvone P, Pardue M. Increased endogenous dopamine prevents myopia in mice. Exp Eye Res. 2020;193:107956.
doi: 10.1016/j.exer.2020.107956
pubmed: 32032629
Huang F, Shu Z, Huang Q, Chen K, Yan W, Wu W, Yang J, Wang Q, Wang F, Zhang C. Retinal dopamine D2 receptors participate in the development of myopia in mice. Invest Ophthalmol Vis Sci. 2022;63(1):24–24.
doi: 10.1167/iovs.63.1.24
pubmed: 35481839
Roy S, Field GD. Dopaminergic modulation of retinal processing from starlight to sunlight. J Pharmacol Sci. 2019;140(1):86–93.
doi: 10.1016/j.jphs.2019.03.006
pubmed: 31109761
Zhou X, Pardue MT, Iuvone PM, Qu J. Dopamine signaling and myopia development: what are the key challenges. Prog Retinal Eye Res. 2017;61:60–71.
doi: 10.1016/j.preteyeres.2017.06.003
Cougnard-Gregoire A, Merle BM, Aslam T, Seddon JM, Aknin I, Klaver CC, Garhöfer G, Layana AG, Minnella AM, Silva R. Blue light exposure: ocular hazards and prevention-a narrative review. Ophthalmol Ther. 2023;12(2):755–88.
doi: 10.1007/s40123-023-00675-3
pubmed: 36808601
pmcid: 9938358
Ouyang X, Yang J, Hong Z, Wu Y, Xie Y, Wang GJB, Pharmacotherapy. Mechanisms of blue light-induced eye hazard and protective measures: a review. Biomed Pharmacother. 2020;130:110577.
doi: 10.1016/j.biopha.2020.110577
pubmed: 32763817
Giannos SA, Kraft ER, Lyons LJ, Gupta PK. Spectral evaluation of eyeglass blocking efficiency of ultraviolet/high-energy visible blue light for ocular protection. Optom Vis Sci. 2019;96(7):513–22.
doi: 10.1097/OPX.0000000000001393
pubmed: 31274740
pmcid: 6615932
Petrowski K, Bührer S, Albus C, Schmalbach B. Increase in cortisol concentration due to standardized bright and blue light exposure on saliva cortisol in the morning following sleep laboratory. Stress. 2021;24(3):331–7.
doi: 10.1080/10253890.2020.1803265
pubmed: 32723201
Downie LE, Wormald R, Evans J, Virgili G, Keller PR, Lawrenson JG, Li T. Analysis of a systematic review about blue light-filtering intraocular lenses for retinal protection: understanding the limitations of the evidence. JAMA Ophthalmol. 2019;137(6):694–7.
doi: 10.1001/jamaophthalmol.2019.0019
pubmed: 30789642
pmcid: 6684842
Abdouh M, Lu M, Chen Y, Goyeneche A, Burnier JV, Burnier MN Jr. Filtering blue light mitigates the deleterious effects induced by the oxidative stress in human retinal pigment epithelial cells. Exp Eye Res. 2022;217:108978.
doi: 10.1016/j.exer.2022.108978
pubmed: 35134392