Design an Energy-Conserving Pathway for Efficient Biosynthesis of 1,5-Pentanediol and 5-Amino-1-Pentanol.
1,5‐pentanediol
5‐amino‐1‐pentanol
Escherichia coli
biosynthesis
energy‐conserving
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
31 Oct 2024
31 Oct 2024
Historique:
revised:
09
10
2024
received:
03
09
2024
accepted:
16
10
2024
medline:
1
11
2024
pubmed:
1
11
2024
entrez:
1
11
2024
Statut:
aheadofprint
Résumé
1,5-Pentanediol (1,5-PDO) is an important five-carbon alcohol, widely used in polymer and pharmaceutical industries. Considering the substantial energy (ATP and NADPH) requirements of previous pathways, an energy-conserving artificial pathway with a higher theoretical yield (0.75 mol/mol glucose) was designed and constructed in this study. In this pathway, lysine is converted into 1,5-PDO by decarboxylation, two transamination, and two reduction reactions. For the purpose of full pathway construction, 5-aminopetanal reductase and 5-amino-1-pentanol (5-APO) transaminase were identified and characterized. By implementing strategies such as modular optimization of gene expression, enhancing lysine biosynthesis and increasing NADPH supply, the engineered strains were able to produce 1502.8 mg/L 5-APO and 726.2 mg/L 1,5-PDO in shake flasks and 11.7 g/L 1,5-PDO in a 3 L bioreactor. This work provides a new and promising pathway for the efficient production of 5-APO and 1,5-PDO.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : This work was supported by the National Key Research and Development Program of China (2022YFC2106100), the National Natural Science Foundation of China (22322802, 22238001, 32271488), and the Fundamental Research Funds for the Central Universities (QNTD2023-01).
Informations de copyright
© 2024 Wiley Periodicals LLC.
Références
Cann, A. F., and J. C. Liao. 2010. “Pentanol Isomer Synthesis in Engineered Microorganisms.” Applied Microbiology and Biotechnology 85, no. 4: 893–899. https://doi.org/10.1007/s00253-009-2262-7.
Cen, X., Y. Liu, B. Chen, D. Liu, and Z. Chen. 2021. “Metabolic Engineering of Escherichia coli for De Novo Production of 1,5‐Pentanediol From Glucose.” ACS Synthetic Biology 10, no. 1: 192–203. https://doi.org/10.1021/acssynbio.0c00567.
Cen, X., Y. Liu, F. Zhu, D. Liu, and Z. Chen. 2022. “Metabolic Engineering of Escherichia coli for High Production of 1,5‐Pentanediol via a Cadaverine‐Derived Pathway.” Metabolic Engineering 74: 168–177. https://doi.org/10.1016/j.ymben.2022.10.012.
Chen, Y., W. Song, G. Wang, et al. 2024. “Metabolic Engineering of High l‐Lysine‐Producing Escherichia coli for De Novo Production of l‐Lysine‐Derived Compounds.” ACS Synthetic Biology 13: 2948–2959. https://doi.org/10.1021/acssynbio.4c00356.
Datsenko, K. A., and B. L. Wanner. 2000. “One‐Step Inactivation of Chromosomal Genes in Escherichia coli K‐12 Using PCR Products.” Proceedings of the National Academy of Sciences of the United States of America 97, no. 12: 6640–6645. https://doi.org/10.1073/pnas.120163297.
Firlotte, N., D. G. Cooper, M. Marić, and J. A. Nicell. 2009. “Characterization of 1,5‐Pentanediol Dibenzoate as a Potential ‘Green’ Plasticizer for Poly(Vinyl Chloride).” Journal of Vinyl and Additive Technology 15, no. 2: 99–107. https://doi.org/10.1002/vnl.20181.
George, K. W., M. G. Thompson, A. Kang, et al. 2015. “Metabolic Engineering for the High‐Yield Production of Isoprenoid‐Based C5 Alcohols in E. coli.” Scientific Reports 5, no. 1: 11128. https://doi.org/10.1038/srep11128.
Kaulmann, U., K. Smithies, M. E. B. Smith, H. C. Hailes, and J. M. Ward. 2007. “Substrate Spectrum of ω‐Transaminase From Chromobacterium violaceum DSM30191 and Its Potential for Biocatalysis.” Enzyme and Microbial Technology 41, no. 5: 628–637. https://doi.org/10.1016/j.enzmictec.2007.05.011.
Larroy, C., M. R. Fernández, E. González, X. Parés, and J. A. Biosca. 2001. “Characterization of the Saccharomyces cerevisiae YMR318C (ADH6) Gene Product as a Broad Specificity NADPH‐Dependent Alcohol Dehydrogenase: Relevance in Aldehyde Reduction.” Biochemical Journal 361, no. 1: 163–172. https://doi.org/10.1042/bj3610163.
Li, W., L. Ma, X. Shen, et al. 2019. “Targeting Metabolic Driving and Intermediate Influx in Lysine Catabolism for High‐Level Glutarate Production.” Nature Communications 10, no. 1: 3337. https://doi.org/10.1038/s41467-019-11289-4.
Liu, S., X. Zhang, F. Liu, et al. 2019. “Designing of a Cofactor Self‐Sufficient Whole‐Cell Biocatalyst System for Production of 1,2‐Amino Alcohols From Epoxides.” ACS Synthetic Biology 8, no. 4: 734–743. https://doi.org/10.1021/acssynbio.8b00364.
Liu, Y., W. Wang, and A. P. Zeng. 2022. “Biosynthesizing Structurally Diverse Diols via a General Route Combining Oxidative and Reductive Formations of OH‐Groups.” Nature Communications 13, no. 1: 1595. https://doi.org/10.1038/s41467-022-29216-5.
Martin, S. F., J. M. Humphrey, A. Ali, and M. C. Hillier. 1999. “Enantioselective Total Syntheses of Ircinal A and Related Manzamine Alkaloids.” Journal of the American Chemical Society 121, no. 4: 866–867. https://doi.org/10.1021/ja9829259.
Rodriguez, G. M., and S. Atsumi. 2014. “Toward Aldehyde and Alkane Production by Removing Aldehyde Reductase Activity in Escherichia coli.” Metabolic Engineering 25: 227–237. https://doi.org/10.1016/j.ymben.2014.07.012.
Rowe, E., B. O. Palsson, and Z. A. King. 2018. “Escher‐FBA: A Web Application for Interactive Flux Balance Analysis.” BMC Systems Biology 12, no. 1: 84. https://doi.org/10.1186/s12918-018-0607-5.
Waegeman, H., J. Beauprez, H. Moens, et al. 2011. “Effect of iclR and arcA Knockouts on Biomass Formation and Metabolic Fluxes in Escherichia coli K12 and Its Implications on Understanding the Metabolism of Escherichia coli BL21 (DE3).” BMC Microbiology 11, no. 1: 70. https://doi.org/10.1186/1471-2180-11-70.
Wang, J., C. Li, Y. Zou, and Y. Yan. 2020. “Bacterial Synthesis of C3‐C5 Diols via Extending Amino Acid Catabolism.” Proceedings of the National Academy of Sciences of the United States of America 117, no. 32: 19159–19167. https://doi.org/10.1073/pnas.2003032117.
Wilding, M., T. S. Peat, J. Newman, and C. Scott. 2016. “A β‐Alanine Catabolism Pathway Containing a Highly Promiscuous ω‐Transaminase in the 12‐Aminododecanate‐Degrading Pseudomonas sp. Strain AAC.” Applied and Environmental Microbiology 82, no. 13: 3846–3856. https://doi.org/10.1128/AEM.00665-16.
Yamamoto, K., and A. Ishihama. 2003. “Two Different Modes of Transcription Repression of the Escherichia coli Acetate Operon by IclR.” Molecular Microbiology 47, no. 1: 183–194. https://doi.org/10.1046/j.1365-2958.2003.03287.x.
Yu, Y., X. Zhu, H. Xu, and X. Zhang. 2019. “Construction of an Energy‐Conserving Glycerol Utilization Pathways for Improving Anaerobic Succinate Production in Escherichia coli.” Metabolic Engineering 56: 181–189. https://doi.org/10.1016/j.ymben.2019.10.002.
Zhang, K., M. R. Sawaya, D. S. Eisenberg, and J. C. Liao. 2008. “Expanding Metabolism for Biosynthesis of Nonnatural Alcohols.” Proceedings of the National Academy of Sciences of the United States of America 105, no. 52: 20653–20658. https://doi.org/10.1073/pnas.0807157106.
Zhao, W., X. Bai, X. Lin, et al. 2023. “Selective and Efficient Production of 1,5‐pentanediol from Tetrahydrofurfuryl Alcohol Using Ni‐La(OH)3 Catalysts.” Fuel 354: 129312. https://doi.org/10.1016/j.fuel.2023.129312.