Conversion of glioma cells into neuron-like cells by small molecules.

Cellular neuroscience Molecular neuroscience Neuroscience

Journal

iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038

Informations de publication

Date de publication:
15 Nov 2024
Historique:
received: 12 03 2024
revised: 25 06 2024
accepted: 29 09 2024
medline: 1 11 2024
pubmed: 1 11 2024
entrez: 1 11 2024
Statut: epublish

Résumé

Currently, researchers are exploring the conversion of astrocytes into functional mature neurons and gradually exploring the conversion of glioma into neurons. We report that SLCDS (SB431542, LDN193189, CHIR99021, DAPT, and SKL2001) has been shown to convert human glioma cells into mature neuron-like cells. The converted cells exhibited upregulation of DCX, TuJ1, MAP2, NeuN, and GAD67, while the expressions of EGFR, PDGFR, Ki67, and vimentin were inhibited. The nTFs, such as NeuroD1 and Sox2, were upregulated, along with TF genes associated with neurogenesis and tumor suppression. We have finally confirmed that overexpressing nTFs can induce the conversion of glioma cells into neuronal cells. This study demonstrates that SLCDS can activate the expression of nTFs in human glioma cells and induce the conversion of human glioma cells into neuron-like cells. Additionally, SLCDS inhibits the expressions of EGFR, PDGFR, Ki67, and Vimentin in gliomas. Our findings offer a potential approach for treating glioma.

Identifiants

DOI: 10.1016/j.isci.2024.111091 PMID: 39483145 PMC: PMC11525470
pubmed: 39483145
doi: 10.1016/j.isci.2024.111091
pii: S2589-0042(24)02316-2
pmc: PMC11525470
doi:

Types de publication

Journal Article

Langues

eng

Pagination

111091

Informations de copyright

© 2024 The Author(s).

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Auteurs

Yongjun Yi (Y)

Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, The Neurosurgery Institute of Guangdong Province, Guangzhou 510632, P.R. China.
Department of Neurosurgery, The First Affiliated Hospital of Jinan University, Guangzhou 510630, P.R. China.

Wenqiang Che (W)

Department of Neurosurgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510080, P.R. China.
Department of Neurosurgery, The First Affiliated Hospital of Jinan University, Guangzhou 510630, P.R. China.

Ping Xu (P)

Department of Orthopedics, The First Affiliated Hospital of Jinan University, Guangzhou 510630, P.R. China.

Chuxiao Mao (C)

Guangdong-Hongkong-Macau CNS Regeneration Institute of Jinan University, Key Laboratory of CNS Regeneration (Jinan University)-Ministry of Education, Guangdong Key Laboratory of Non-human Primate Research, Guangzhou 510630, P.R. China.

Zhizhong Li (Z)

Department of Orthopedics, The First Affiliated Hospital of Jinan University, Guangzhou 510630, P.R. China.

Qingsong Wang (Q)

Guangdong-Hongkong-Macau CNS Regeneration Institute of Jinan University, Key Laboratory of CNS Regeneration (Jinan University)-Ministry of Education, Guangdong Key Laboratory of Non-human Primate Research, Guangzhou 510630, P.R. China.

Jun Lyu (J)

Department of Clinical Research, The First Affiliated Hospital of Jinan University, Guangzhou 510632, P.R. China.

Xiangyu Wang (X)

Department of Neurosurgery, The First Affiliated Hospital of Jinan University, Guangzhou 510630, P.R. China.

Classifications MeSH