Titre : Cellules THP-1

Cellules THP-1 : Questions médicales fréquentes

Termes MeSH sélectionnés :

Observer Variation
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Cellules THP-1", "description": "Comment identifier les cellules THP-1 en laboratoire ?\nQuels tests sont utilisés pour caractériser les cellules THP-1 ?\nLes cellules THP-1 peuvent-elles être contaminées ?\nQuelle est la source des cellules THP-1 ?\nLes cellules THP-1 sont-elles sensibles à des médicaments ?", "url": "https://questionsmedicales.fr/mesh/D000074084?mesh_terms=Observer+Variation&page=5#section-diagnostic" }, { "@type": "MedicalWebPage", "name": "Symptômes", "headline": "Symptômes sur Cellules THP-1", "description": "Quels sont les marqueurs d'activation des cellules THP-1 ?\nLes cellules THP-1 montrent-elles des signes d'inflammation ?\nComment les cellules THP-1 réagissent-elles aux pathogènes ?\nLes cellules THP-1 peuvent-elles se différencier ?\nQuels effets ont les cytokines sur les cellules THP-1 ?", "url": "https://questionsmedicales.fr/mesh/D000074084?mesh_terms=Observer+Variation&page=5#section-symptômes" }, { "@type": "MedicalWebPage", "name": "Prévention", 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"https://questionsmedicales.fr/mesh/D000074084?mesh_terms=Observer+Variation&page=5#section-traitements" }, { "@type": "MedicalWebPage", "name": "Complications", "headline": "Complications sur Cellules THP-1", "description": "Quelles complications peuvent survenir lors de l'utilisation des cellules THP-1 ?\nLes cellules THP-1 peuvent-elles induire des réponses immunitaires indésirables ?\nQuels risques sont associés à la manipulation des cellules THP-1 ?\nLes cellules THP-1 peuvent-elles être affectées par des agents externes ?\nComment minimiser les complications lors des expériences avec THP-1 ?", "url": "https://questionsmedicales.fr/mesh/D000074084?mesh_terms=Observer+Variation&page=5#section-complications" }, { "@type": "MedicalWebPage", "name": "Facteurs de risque", "headline": "Facteurs de risque sur Cellules THP-1", "description": "Quels facteurs influencent la croissance des cellules THP-1 ?\nLes cellules THP-1 sont-elles sensibles à des agents environnementaux ?\nQuels traitements peuvent affecter les cellules THP-1 ?\nLes cellules THP-1 sont-elles affectées par le stress oxydatif ?\nQuels facteurs génétiques influencent les cellules THP-1 ?", "url": "https://questionsmedicales.fr/mesh/D000074084?mesh_terms=Observer+Variation&page=5#section-facteurs de risque" } ] }, { "@type": "FAQPage", "mainEntity": [ { "@type": "Question", "name": "Comment identifier les cellules THP-1 en laboratoire ?", "position": 1, "acceptedAnswer": { "@type": "Answer", "text": "Les cellules THP-1 sont identifiées par leur morphologie et leur expression de marqueurs spécifiques." } }, { "@type": "Question", "name": "Quels tests sont utilisés pour caractériser les cellules THP-1 ?", "position": 2, "acceptedAnswer": { "@type": "Answer", "text": "Des tests comme la cytométrie en flux et l'immunofluorescence sont utilisés." } }, { "@type": "Question", "name": "Les cellules THP-1 peuvent-elles être contaminées ?", "position": 3, "acceptedAnswer": { "@type": "Answer", "text": "Oui, comme toutes les lignées cellulaires, elles peuvent être contaminées par des bactéries ou des mycoplasmes." } }, { "@type": "Question", "name": "Quelle est la source des cellules THP-1 ?", "position": 4, "acceptedAnswer": { "@type": "Answer", "text": "Les cellules THP-1 proviennent de la moelle osseuse d'un patient atteint de leucémie." } }, { "@type": "Question", "name": "Les cellules THP-1 sont-elles sensibles à des médicaments ?", "position": 5, "acceptedAnswer": { "@type": "Answer", "text": "Oui, elles sont souvent utilisées pour tester la sensibilité à divers agents pharmacologiques." } }, { "@type": "Question", "name": "Quels sont les marqueurs d'activation des cellules THP-1 ?", "position": 6, "acceptedAnswer": { "@type": "Answer", "text": "Les marqueurs incluent CD14, CD11b et CD68, indiquant leur activation." } }, { "@type": "Question", "name": "Les cellules THP-1 montrent-elles des signes d'inflammation ?", "position": 7, "acceptedAnswer": { "@type": "Answer", "text": "Oui, elles produisent des cytokines pro-inflammatoires lorsqu'elles sont activées." } }, { "@type": "Question", "name": "Comment les cellules THP-1 réagissent-elles aux pathogènes ?", "position": 8, "acceptedAnswer": { "@type": "Answer", "text": "Elles phagocytent les pathogènes et sécrètent des médiateurs inflammatoires." } }, { "@type": "Question", "name": "Les cellules THP-1 peuvent-elles se différencier ?", "position": 9, "acceptedAnswer": { "@type": "Answer", "text": "Oui, elles peuvent se différencier en macrophages sous l'influence de certains facteurs." } }, { "@type": "Question", "name": "Quels effets ont les cytokines sur les cellules THP-1 ?", "position": 10, "acceptedAnswer": { "@type": "Answer", "text": "Les cytokines modulent leur activation et leur capacité à produire d'autres médiateurs." } }, { "@type": "Question", "name": "Comment prévenir la contamination des cellules THP-1 ?", "position": 11, "acceptedAnswer": { "@type": "Answer", "text": "Utiliser des techniques aseptiques et vérifier régulièrement la pureté cellulaire." } }, { "@type": "Question", "name": "Quelles conditions de culture sont idéales pour les cellules THP-1 ?", "position": 12, "acceptedAnswer": { "@type": "Answer", "text": "Elles nécessitent un milieu riche en nutriments et une température contrôlée." } }, { "@type": "Question", "name": "Les cellules THP-1 nécessitent-elles des soins particuliers ?", "position": 13, "acceptedAnswer": { "@type": "Answer", "text": "Oui, elles doivent être manipulées avec précaution pour éviter le stress cellulaire." } }, { "@type": "Question", "name": "Comment assurer la viabilité des cellules THP-1 ?", "position": 14, "acceptedAnswer": { "@type": "Answer", "text": "Maintenir des conditions optimales de culture et éviter les cycles de congélation-décongélation." } }, { "@type": "Question", "name": "Les cellules THP-1 peuvent-elles être cryogénisées ?", "position": 15, "acceptedAnswer": { "@type": "Answer", "text": "Oui, elles peuvent être cryogénisées pour une conservation à long terme." } }, { "@type": "Question", "name": "Comment les cellules THP-1 sont-elles utilisées en recherche ?", "position": 16, "acceptedAnswer": { "@type": "Answer", "text": "Elles sont utilisées pour étudier les mécanismes immunitaires et tester des médicaments." } }, { "@type": "Question", "name": "Peut-on modifier génétiquement les cellules THP-1 ?", "position": 17, "acceptedAnswer": { "@type": "Answer", "text": "Oui, des techniques comme CRISPR peuvent être utilisées pour modifier leur génome." } }, { "@type": "Question", "name": "Quels traitements sont testés sur les cellules THP-1 ?", "position": 18, "acceptedAnswer": { "@type": "Answer", "text": "Des traitements anti-inflammatoires et anticancéreux sont souvent testés." } }, { "@type": "Question", "name": "Les cellules THP-1 peuvent-elles être utilisées pour des tests de toxicité ?", "position": 19, "acceptedAnswer": { "@type": "Answer", "text": "Oui, elles sont souvent utilisées pour évaluer la toxicité des composés chimiques." } }, { "@type": "Question", "name": "Comment les cellules THP-1 aident-elles à comprendre les maladies ?", "position": 20, "acceptedAnswer": { "@type": "Answer", "text": "Elles modélisent des maladies inflammatoires et infectieuses pour étudier les mécanismes." } }, { "@type": "Question", "name": "Quelles complications peuvent survenir lors de l'utilisation des cellules THP-1 ?", "position": 21, "acceptedAnswer": { "@type": "Answer", "text": "Des résultats biaisés peuvent survenir si les cellules sont contaminées ou mal caractérisées." } }, { "@type": "Question", "name": "Les cellules THP-1 peuvent-elles induire des réponses immunitaires indésirables ?", "position": 22, "acceptedAnswer": { "@type": "Answer", "text": "Oui, elles peuvent parfois produire des réponses immunitaires non spécifiques." } }, { "@type": "Question", "name": "Quels risques sont associés à la manipulation des cellules THP-1 ?", "position": 23, "acceptedAnswer": { "@type": "Answer", "text": "Le risque principal est la contamination croisée avec d'autres lignées cellulaires." } }, { "@type": "Question", "name": "Les cellules THP-1 peuvent-elles être affectées par des agents externes ?", "position": 24, "acceptedAnswer": { "@type": "Answer", "text": "Oui, des agents comme les toxines ou les médicaments peuvent altérer leur fonction." } }, { "@type": "Question", "name": "Comment minimiser les complications lors des expériences avec THP-1 ?", "position": 25, "acceptedAnswer": { "@type": "Answer", "text": "Suivre des protocoles stricts et effectuer des contrôles réguliers de qualité." } }, { "@type": "Question", "name": "Quels facteurs influencent la croissance des cellules THP-1 ?", "position": 26, "acceptedAnswer": { "@type": "Answer", "text": "Des facteurs comme la température, le pH et la composition du milieu influencent leur croissance." } }, { "@type": "Question", "name": "Les cellules THP-1 sont-elles sensibles à des agents environnementaux ?", "position": 27, "acceptedAnswer": { "@type": "Answer", "text": "Oui, elles peuvent être affectées par des agents chimiques ou physiques dans leur environnement." } }, { "@type": "Question", "name": "Quels traitements peuvent affecter les cellules THP-1 ?", "position": 28, "acceptedAnswer": { "@type": "Answer", "text": "Des traitements comme les cytokines ou les médicaments peuvent moduler leur activité." } }, { "@type": "Question", "name": "Les cellules THP-1 sont-elles affectées par le stress oxydatif ?", "position": 29, "acceptedAnswer": { "@type": "Answer", "text": "Oui, le stress oxydatif peut altérer leur fonction et leur viabilité." } }, { "@type": "Question", "name": "Quels facteurs génétiques influencent les cellules THP-1 ?", "position": 30, "acceptedAnswer": { "@type": "Answer", "text": "Des variations génétiques peuvent affecter leur réponse aux stimuli immunitaires." } } ] } ] }

Sources (10000 au total)

A 10-Year Review of Intraoral Salivary Gland Tumor Diagnoses: Diagnostic Challenges and Inter-Observer Agreement.

Salivary gland tumors (SGT) are a diverse group of neoplasms arising from the major and minor glands. The oral cavity is the most common site for minor SGT (IMSGT), and these lesions frequently pose a... We conducted a retrospective analysis of 248 IMSGT received between 2010 and 2019. We evaluated the diagnostic challenge of the cases by stratifying according to whether a definitive, favored, or inde... Of the 248 biopsies, 191 had a definitive diagnosis, 38 favored diagnoses, and 19 were indeterminate. The predominant diagnoses considered for the indeterminate category were pleomorphic adenoma/myoep... Diagnostic challenges in IMSGT incisional biopsies were infrequent, especially if multiple pathologists were consulted. PA, PAC, AdCC, and adenocarcinoma NOS were the histologic types more commonly po...

Intra- and Inter-observer Agreement of High Resolution Antroduodenal Manometry in Pediatric Patients Among Single Center Experts.

Studies in adults have suggested that high-resolution technology increases the diagnostic yield of antroduodenal manometry (ADM). However, there is no study comparing high-resolution with low-resoluti... Twenty-four anonymized ADM studies were blindly analyzed by 3 experienced pediatric neurogastroenterologists. All studies had been performed using a low-compliance water-perfused system with a 20-chan... Analysis with high-resolution CLP revealed a substantial inter-observer agreement among the 3 observers regarding the diagnosis (Krippendorff's alpha: 0.832; average pairwise percentage agreement: 88.... Our study demonstrated higher diagnostic accuracy for high-resolution ADM compared to the low-resolution recordings. However, although it is well established for other motility investigations, PTP is ...

Evaluation of inter-observer variability regarding aortic and mitral valve findings on transesophageal echocardiograms ordered for suspected endocarditis.

Transesophageal echocardiography (TEE) is the gold standard for the detection of valvular vegetations (VV). Differentiating small VV from degenerative changes is challenging and prone to inter-observe... A total of 349 consecutive TEEs were evaluated. Studies were classified as "definite, possible, or no" IE with valve masses classified further by morphology. Nine faculty echocardiographers scored ran... Positive blood cultures were present in 81% and 45% had definite IE by the modified Duke criteria. There was moderate reader agreement regarding the presence of a valvular mass for both the AV (Kf = .... In a large academic center, the inter-observer agreement for the presence of a left sided valvular mass was moderate and agreement regarding the final diagnosis of IE was fair to moderate, with better...

Adaptation of a channelized Hotelling observer model to accommodate anthropomorphic backgrounds and moving test objects in X-ray angiography.

Prior implementations of the channelized Hotelling observer (CHO) model have succeeded in assessing the performance of X-ray angiography systems under a variety of imaging conditions. However, often t... This work builds up on prior established CHO methods and introduces a new approach to switch from the already established "multiple-sample" CHO implementation to a "single-sample" technique. The propo... To assess the proposed method, two image data sets were acquired with a clinical X-ray angiography system. The first set consisted of a uniform background in combination with static test objects while... For the uniform background data set, considering all detectability indices greater or equal than 1, the ratio between the detectability indices of the proposed single-sample approach versus the multip... A new single-sample variant of the CHO model to assess the performance of X-ray angiography imaging systems is proposed. The new approach is consistent with quantum-limited image quality theory and wi...

A standardized method to measure the membranous urethral length (MUL) on MRI of the prostate with high inter- and intra-observer agreement.

The membranous urethral length (MUL), defined as the length between the apex and penile base as measured on preoperative prostate magnetic resonance imaging (MRI), is an important predictor for urinar... Prostate cancer patients underwent a preoperative MRI and robot-assisted radical prostatectomy (RARP) at one high-volume RARP center. MUL measurement was based on well-defined landmarks on sagittal T2... The initial inter-observer agreement was ICC 0.63; 95% confidence interval (CI) 0.28-0.81. Radiologist 3 measured the MUL mean 3.9 mm (SD 3.3) longer than the other readers, interpreting the caudal po... The MUL can be measured reliably with high agreement among radiologists.... • After discussion on the correct anatomical definition, the inter- and intra - observer agreements of membranous urethral length (MUL) measurement on magnetic resonance imaging (MRI) were high. • A r...

Assessment of the reliability of virtual preanesthetic airway evaluation compared to traditional in-person airway evaluation: a prospective observational study.

Airway evaluation is a fundamental component of the preanesthetic examination. Virtual care has increased during the COVID-19 pandemic. We aimed to assess the reliability of a virtual preanesthetic ai... This prospective observational study compared the inter-rater agreement of an in-person airway evaluation performed by a consultant anesthesiologist with a virtual airway evaluation (VAE) performed by... One hundred out of 111 participants completed all three evaluations. The in-person anesthesiologist airway evaluations had fair and good levels of agreement of total scores with the VAEs of the anesth... Virtual airway evaluations performed by anesthesiologists and medical students had fair and good inter-rater agreement, respectively, with in-person anesthesiologist airway evaluations. Further study ...

Inter-observer variability in library plan selection on iterative CBCT and synthetic CT images of cervical cancer patients.

In the Library-of-Plans (LoP) approach, correct plan selection is essential for delivering radiotherapy treatment accurately. However, poor image quality of the cone-beam computed tomography (CBCT) ma... CBCT images of 12 patients were used to investigate the inter-observer variability of plan selection based on different CBCT image types. Six observers were asked to individually select a plan based o... The agreement between observers was significantly higher on sCT compared to CBCT. The consensus meeting improved the duration and inter-observer variability. In this manuscript, the guidelines attribu... The use of sCTs can increase the agreement of plan selection among observers and the gold standard was indicated to be selected more often. It is important that clear guidelines for plan selection are...

A comparative study of the inter-observer variability on Gleason grading against Deep Learning-based approaches for prostate cancer.

Among all the cancers known today, prostate cancer is one of the most commonly diagnosed in men. With modern advances in medicine, its mortality has been considerably reduced. However, it is still a l... In this work, the inter-observer variability of a local dataset of 80 whole-slide images annotated by a team of 5 pathologists from the same group was analyzed at both area and label level. Four appro... An inter-observer variability of 0.6946 κ was obtained, with 46% discrepancy in terms of area size of the annotations performed by the pathologists. The best trained models achieved 0.826±0.014κ on th... The obtained results show that deep learning-based automatic diagnosis systems could help reduce the widely-known inter-observer variability that is present among pathologists and support them in thei...