Microdissection au laser : Questions médicales fréquentes
Questions fréquentes et termes MeSH associés
Diagnostic 5
Comment la microdissection au laser est-elle utilisée en diagnostic ?
Quels types de tissus peuvent être analysés ?
La microdissection au laser est-elle invasive ?
Quels marqueurs sont utilisés pour identifier les cellules ?
Peut-on utiliser cette technique pour le cancer ?
Symptômes 5
Quels symptômes peuvent nécessiter une microdissection au laser ?
La microdissection peut-elle aider à identifier des maladies ?
Quels signes cliniques sont associés aux tissus analysés ?
Les symptômes sont-ils toujours visibles avant la microdissection ?
Comment les symptômes influencent-ils le choix de la technique ?
Prévention 5
La microdissection au laser aide-t-elle à la prévention des maladies ?
Quels facteurs de risque peuvent être identifiés ?
Peut-on utiliser cette technique pour le dépistage ?
Comment la prévention est-elle améliorée par cette technique ?
La microdissection peut-elle aider à la recherche préventive ?
Traitements 5
La microdissection au laser est-elle un traitement ?
Comment les résultats influencent-ils le traitement ?
Peut-on combiner cette technique avec d'autres traitements ?
Quels traitements peuvent être guidés par cette technique ?
La microdissection aide-t-elle à évaluer l'efficacité des traitements ?
Complications 5
Quelles complications peuvent survenir avec cette technique ?
La microdissection au laser peut-elle causer des dommages tissulaires ?
Y a-t-il des risques d'infection ?
Comment éviter les complications lors de l'utilisation ?
Les complications affectent-elles les résultats ?
Facteurs de risque 5
Quels facteurs de risque sont associés à l'utilisation de cette technique ?
La génétique joue-t-elle un rôle dans l'indication de cette technique ?
L'environnement influence-t-il les risques associés ?
Les habitudes de vie affectent-elles les risques ?
Comment évaluer les facteurs de risque avant la microdissection ?
Contenu validé par Dr Olivier Menir
Expert en Médecine, Optimisation des Parcours de Soins et Révision Médicale
Validation scientifique effectuée le 14/04/2025
Contenu vérifié selon les dernières recommandations médicales
Auteurs principaux
Vibhav Gautam
3 publications dans cette catégorie
- National Institute of Plant Genome Research (NIPGR), New Delhi, India.
-
Improved Method of RNA Isolation from Laser Capture Microdissection (LCM)-Derived Plant Tissues.
Methods in molecular biology (Clifton, N.J.)
-
Single Cell Type Specific RNA Isolation and Gene Expression Analysis in Rice Using Laser Capture Microdissection (LCM)-Based Method.
Methods in molecular biology (Clifton, N.J.)
-
Improved laser capture microdissection (LCM)-based method for isolation of RNA, including miRNA and expression analysis in woody apple bud meristem.
Planta 2019-04-11
Ananda K Sarkar
3 publications dans cette catégorie
- National Institute of Plant Genome Research (NIPGR), New Delhi, India. aksarkar@nipgr.ac.in.
-
Improved Method of RNA Isolation from Laser Capture Microdissection (LCM)-Derived Plant Tissues.
Methods in molecular biology (Clifton, N.J.)
-
Single Cell Type Specific RNA Isolation and Gene Expression Analysis in Rice Using Laser Capture Microdissection (LCM)-Based Method.
Methods in molecular biology (Clifton, N.J.)
-
Improved laser capture microdissection (LCM)-based method for isolation of RNA, including miRNA and expression analysis in woody apple bud meristem.
Planta 2019-04-11
Yinggai Song
2 publications dans cette catégorie
- Department of Dermatology and Venereology, National Clinical Research Center for Skin and Immune Diseases, Research Center for Medical Mycology, Beijing Key Laboratory of Molecular Diagnosis on Dermatoses, Peking University First Hospital, Beijing, People's Republic of China.
-
Application of laser capture microdissection and PCR sequencing in the diagnosis of
Emerging microbes & infections
-
Disseminated Cryptococcosis Presenting as Cellulitis Diagnosed by Laser Capture Microdissection: A Case Report and Literature Review.
Mycopathologia 2021-04-03
Ruoyu Li
2 publications dans cette catégorie
- Department of Dermatology and Venereology, National Clinical Research Center for Skin and Immune Diseases, Research Center for Medical Mycology, Beijing Key Laboratory of Molecular Diagnosis on Dermatoses, Peking University First Hospital, Beijing, People's Republic of China.
-
Application of laser capture microdissection and PCR sequencing in the diagnosis of
Emerging microbes & infections
-
Disseminated Cryptococcosis Presenting as Cellulitis Diagnosed by Laser Capture Microdissection: A Case Report and Literature Review.
Mycopathologia 2021-04-03
Wei Liu
2 publications dans cette catégorie
- Department of Dermatology and Venereology, National Clinical Research Center for Skin and Immune Diseases, Research Center for Medical Mycology, Beijing Key Laboratory of Molecular Diagnosis on Dermatoses, Peking University First Hospital, Beijing, People's Republic of China.
-
Application of laser capture microdissection and PCR sequencing in the diagnosis of
Emerging microbes & infections
-
Application of laser capture microdissection and polymerase chain reaction in the diagnosis of Trichoderma longibrachiatum infection: a promising diagnostic tool for 'fungal contaminants' infection.
Medical mycology
Jingjing Dong
2 publications dans cette catégorie
- Guangdong Provincial Engineering Research Center of Autoimmune Disease Precision Medicine, Shenzhen Engineering Research Center of Autoimmune Disease, Shenzhen Clinical Research Center for Geriatrics, Shenzhen People' s Hospital, The Second Clinical Medical College, Jinan University, Shenzhen 518020, China; Department of Nephrology, The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou 510632, China.
-
Proteomic profiling of laser capture microdissection kidneys from diabetic nephropathy patients.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2024-07-05
-
Global-feature of autoimmune glomerulonephritis using proteomic analysis of laser capture microdissected glomeruli.
Frontiers in immunology 2023-03-22
Fanna Liu
2 publications dans cette catégorie
- Department of Nephrology, The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou 510632, China. Electronic address: lfn-au@126.com.
-
Proteomic profiling of laser capture microdissection kidneys from diabetic nephropathy patients.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2024-07-05
-
Global-feature of autoimmune glomerulonephritis using proteomic analysis of laser capture microdissected glomeruli.
Frontiers in immunology 2023-03-22
Donge Tang
2 publications dans cette catégorie
- Guangdong Provincial Engineering Research Center of Autoimmune Disease Precision Medicine, Shenzhen Engineering Research Center of Autoimmune Disease, Shenzhen Clinical Research Center for Geriatrics, Shenzhen People' s Hospital, The Second Clinical Medical College, Jinan University, Shenzhen 518020, China. Electronic address: donge66@126.com.
-
Proteomic profiling of laser capture microdissection kidneys from diabetic nephropathy patients.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2024-07-05
-
Global-feature of autoimmune glomerulonephritis using proteomic analysis of laser capture microdissected glomeruli.
Frontiers in immunology 2023-03-22
Yong Dai
2 publications dans cette catégorie
- Comprehensive Health Industry Research Center, Taizhou Research Institute, Southern University of Science and Technology, Taizhou 317000, China; The First Affiliated Hospital, School of Medicine, Anhui University of Science and Technology, Huainan 232001, Anhui, China. Electronic address: daiyong22@aliyun.com.
-
Proteomic profiling of laser capture microdissection kidneys from diabetic nephropathy patients.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2024-07-05
-
Global-feature of autoimmune glomerulonephritis using proteomic analysis of laser capture microdissected glomeruli.
Frontiers in immunology 2023-03-22
Karine Gousset
2 publications dans cette catégorie
- Biology Department, California State University Fresno, 2555 East San Ramon Ave M/S SB73, Fresno, CA 93740, USA. kgousset@csufresno.edu.
-
A novel Microproteomic Approach Using Laser Capture Microdissection to Study Cellular Protrusions.
International journal of molecular sciences 2019-03-07
-
Utilization of Laser Capture Microdissection Coupled to Mass Spectrometry to Uncover the Proteome of Cellular Protrusions.
Methods in molecular biology (Clifton, N.J.)
Ana Gordon
2 publications dans cette catégorie
- Biology Department, California State University Fresno, 2555 East San Ramon Ave M/S SB73, Fresno, CA 93740, USA. anagordonbc@gmail.com.
-
A novel Microproteomic Approach Using Laser Capture Microdissection to Study Cellular Protrusions.
International journal of molecular sciences 2019-03-07
-
Utilization of Laser Capture Microdissection Coupled to Mass Spectrometry to Uncover the Proteome of Cellular Protrusions.
Methods in molecular biology (Clifton, N.J.)
Suchitra Chavan
2 publications dans cette catégorie
- Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina.
-
Fixation and Laser Capture Microdissection of Plant Tissue for RNA Extraction and RNASeq Library Preparation.
Current protocols
-
Laser capture microdissection transcriptome reveals spatiotemporal tissue gene expression patterns of M. truncatula roots responding to rhizobia.
Molecular plant-microbe interactions : MPMI 2023-09-17
Elise Schnabel
2 publications dans cette catégorie
- Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina.
-
Fixation and Laser Capture Microdissection of Plant Tissue for RNA Extraction and RNASeq Library Preparation.
Current protocols
-
Laser capture microdissection transcriptome reveals spatiotemporal tissue gene expression patterns of M. truncatula roots responding to rhizobia.
Molecular plant-microbe interactions : MPMI 2023-09-17
Julia Frugoli
2 publications dans cette catégorie
- Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina.
-
Fixation and Laser Capture Microdissection of Plant Tissue for RNA Extraction and RNASeq Library Preparation.
Current protocols
-
Laser capture microdissection transcriptome reveals spatiotemporal tissue gene expression patterns of M. truncatula roots responding to rhizobia.
Molecular plant-microbe interactions : MPMI 2023-09-17
Hua Li
2 publications dans cette catégorie
- State Key Laboratory of Oncogenes and Related Genes, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
-
Robust Acquisition of Spatial Transcriptional Programs in Tissues With Immunofluorescence-Guided Laser Capture Microdissection.
Frontiers in cell and developmental biology 2022-03-25
-
Spatial proteome profiling by immunohistochemistry-based laser capture microdissection and data-independent acquisition proteomics.
Analytica chimica acta 2020-07-08
Swati Verma
2 publications dans cette catégorie
- National Institute of Plant Genome Research (NIPGR), New Delhi, India.
-
Improved Method of RNA Isolation from Laser Capture Microdissection (LCM)-Derived Plant Tissues.
Methods in molecular biology (Clifton, N.J.)
-
Improved laser capture microdissection (LCM)-based method for isolation of RNA, including miRNA and expression analysis in woody apple bud meristem.
Planta 2019-04-11
Elisa Baldelli
2 publications dans cette catégorie
- Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, VA, USA.
-
Analysis of neuroendocrine clones in NSCLCs using an immuno-guided laser-capture microdissection-based approach.
Cell reports methods 2022-08-22
-
The impact of ultraviolet- and infrared-based laser microdissection technology on phosphoprotein detection in the laser microdissection-reverse phase protein array workflow.
Clinical proteomics 2020-03-09
Emanuel F Petricoin
2 publications dans cette catégorie
- Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, VA, USA.
- School of Systems Biology, George Mason University, Manassas, VA, USA.
-
Analysis of neuroendocrine clones in NSCLCs using an immuno-guided laser-capture microdissection-based approach.
Cell reports methods 2022-08-22
-
The impact of ultraviolet- and infrared-based laser microdissection technology on phosphoprotein detection in the laser microdissection-reverse phase protein array workflow.
Clinical proteomics 2020-03-09
Mariaelena Pierobon
2 publications dans cette catégorie
- Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, VA, USA.
- School of Systems Biology, George Mason University, Manassas, VA, USA.
-
Analysis of neuroendocrine clones in NSCLCs using an immuno-guided laser-capture microdissection-based approach.
Cell reports methods 2022-08-22
-
The impact of ultraviolet- and infrared-based laser microdissection technology on phosphoprotein detection in the laser microdissection-reverse phase protein array workflow.
Clinical proteomics 2020-03-09
Leonie Selbach
1 publication dans cette catégorie
- Department of Computer Science, Faculty of Mathematics, Ruhr University Bochum, Bochum, Germany. leonie.selbach@rub.de.
- Center for Protein Diagnostics, Ruhr University Bochum, Bochum, Germany. leonie.selbach@rub.de.
-
Shape decomposition algorithms for laser capture microdissection.
Algorithms for molecular biology : AMB 2021-07-08
Sources (10000 au total)
Cultural Differences in Patients' Preferences for Paternalism: Comparing Mexican and American Patients' Preferences for and Experiences with Physician Paternalism and Patient Autonomy.
Following up on previous research demonstrating the high level of care realized by a paternalistic Mexican physician, the present research further explored the hypothesis that there are cultural diffe...
A stated preference survey to explore patient preferences for novel preventive migraine treatments.
The objective of this study was to explore patient preference for attributes of calcitonin gene-related peptide (CGRP) inhibitors for the preventive treatment of migraine and to describe differences i... CGRP inhibitors are a novel class of migraine drugs specifically developed for the preventive treatment of migraine. Clinicians should understand patient preferences for CGRP inhibitors to inform and ... Patients with migraine in the US and Germany were recruited to participate in an online discrete choice experiment (DCE) survey, which presented hypothetical treatment choices using five attributes: m... Of 680 who consented to participate, 506 participants completed the survey and were included in the study (US = 257; Germany = 249). Overall, participants placed highest importance (preference weight,... In this DCE assessing CGRP inhibitors attributes, the main driver of patient choice was treatment effectiveness, specifically reduced migraine severity, and consistent treatment effectiveness. Further...
Patient-Preference Diagnostics: Adapting Stated-Preference Methods to Inform Effective Shared Decision Making.
While clinical practice guidelines underscore the need to incorporate patient preferences in clinical decision making, incorporating meaningful assessment of patient preferences in clinical encounters... We propose an approach to efficiently diagnose preferences of patients for outcomes of treatment alternatives by leveraging prior information on patient preferences to generate adaptive choice questio... We identified 4 classes representing distinct preference profiles for patients who participated in a previous first-time anterior shoulder dislocation (FTASD) survey. Posterior probabilities of class ... Our results suggest that this approach could help diagnose patient preferences for treatments for a condition such as FTASD with acceptable precision using as few as 2 choice questions. Such preferenc... Approaches that combine patient preferences and clinical evidence can facilitate effective patient-provider communication and more patient-centric healthcare decisions. However, diagnosing individual-...
Patient Preferences for Lung Cancer Interception Therapy.
Interception therapy requires individuals to undergo treatment to prevent a future medical event, but little is known about preferences of individuals at high risk for lung cancer and whether they wou... To explore preferences of individuals at high risk for lung cancer for potential interception therapies to reduce this risk.... This survey study used a discrete-choice experiment and included hypothetical lung cancer interception treatments with 4 attributes: reduction in lung cancer risk over 3 years, injection site reaction... Attribute-level preference weights were estimated, and conditional relative attribute importance, maximum acceptable risks, and minimum acceptable benefits were calculated. Characteristics of responde... Of the 803 survey respondents, 495 (61.6%) were female, 138 (17.2%) were African American or Black, 55 (6.8%) were Alaska Native, American Indian, or Native American, 44 (5.5%) were Asian or Native Ha... In this survey study of individuals at high risk of lung cancer, most respondents were willing to consider interception therapy. These results suggest the importance of benefit-risk assessments for fu...
Adaptation of the WOMAC for Use in a Patient Preference Study.
To adapt a patient-reported outcome (PRO) measure, the Western Ontario McMaster Universities Osteoarthritis Index (WOMAC), into efficacy attributes for a discrete choice experiment (DCE) survey design... The adaptation comprised four steps: (1) selecting domains of interest; (2) determining presentation and framing of selected attributes; (3) determining attribute levels; and (4) developing choice tas... The WOMAC pain and function domains were selected for adaption to two efficacy attributes. Two versions of the discrete choice experiment (DCE) instrument were created to compare efficacy using (1) to... This study adds to the growing literature regarding adapting PRO measures for patient preference studies. Such adaptation is important for designing a preference study that can incorporate a clinical ...
Patient Preferences for Waiting Time and Kidney Quality.
Approximately 20% of deceased donor kidneys are discarded each year in the United States. Some of these kidneys could benefit patients who are waitlisted. Understanding patient preferences regarding a... This study uses a discrete choice experiment that presents a deceased donor kidney to patients who are waiting for, or have received, a kidney transplant. The choices involve trade-offs between accept... In total, 605 participants completed the discrete choice experiment. Respondents made trade-offs between kidney quality and waiting time. The average respondent would accept a kidney today, with 6.5 y... Participants preferred accepting a lower-quality kidney in return for shorter waiting time, particularly those who were older and had lower functional status....
Preference of acromegaly patients for treatment attributes in Spain.
Acromegaly is a rare disease caused by increased growth hormone secretion and a subsequent increase in insulin-like growth factor I (IGF-I) levels. Patients display multiple comorbidities that affect ... A cross-sectional study based on interviews and a discrete choice experiment (DCE) in a Spanish cohort.... Adult patients diagnosed with acromegaly ≥1 year before the start of the study and under treatment were included. Treatment attributes were collected from patient testimony during face-to-face intervi... Sixty-seven patients completed the study. QoL improvement was the most important treatment attribute (37%), followed by IGF-I control (20%), blood sugar control (17%) and tumour control (13%). Seconda... QoL greatly influences patient treatment preference. Since acromegaly patients are informed and aware of their disease, treatment choices should always be shared with patients....
How culture influences patient preferences for patient-centered care with their doctors.
Patient-centered care (PCC) is the prevailing model of care globally. However, most research on PCC has been conducted in Westernized countries or has focused on only two facets of PCC: decision-makin... Participants (... Participants from all four countries had similar preferences for empathy and shared decision-making. For other facets of PCC, participants in the Philippines and Australia expressed somewhat similar p... Empathy, information exchange, and shared decision-making are values shared across countries, while preferences for how the information is shared, and the importance of the doctor-patient relationship...
Understanding treatment preferences and cognitive outcomes in patients with gliomas.
Understanding how glioma patients value cognitive outcomes is essential to personalizing their treatment plans. The purpose of this study was to identify the modifiable cognitive functions most affect... Patients with gliomas were prospectively enrolled in focus groups and individual interviews using a standardized guide focusing on cognitive functions until saturation was achieved. Patient values and... Twenty participants participated, of whom 60% were female. Racial identification consisted of 75% White, 15% Black/African American, and 10% Other Racial Identification. The cognitive functions most e... Glioma patients in this study prioritized cognitive functions according to memory, personal identity, and their ability to communicate with loved ones independently of experiencing deficits in these f...