Identification, expression and function of myosin heavy chain family genes in Tribolium castaneum.

Animals Gene Expression Regulation, Developmental Insect Proteins / chemistry Multigene Family Myosin Heavy Chains / chemistry Pupa / genetics RNA, Messenger / genetics Tribolium / genetics
Mhc Myo20 Myosin RNAi Tribolium castaneum

Journal

Genomics
ISSN: 1089-8646
Titre abrégé: Genomics
Pays: United States
ID NLM: 8800135

Informations de publication

Date de publication:
07 2019
Historique:
received: 26 09 2017
revised: 12 04 2018
accepted: 13 04 2018
pubmed: 21 4 2018
medline: 31 12 2019
entrez: 21 4 2018
Statut: ppublish

Résumé

Functions of myosin heavy chain (myosin) family genes are poorly understood in some insects. To address this, we determined the expression and function of myosin family genes in Tribolium castaneum. TcMyo15 is predominantly expressed in early embryos, late larvae and early adults, but TcMyo7B transcripts significantly increased in late larvae. TcMyo20 transcripts are abundant in pre-adults, whereas TcMhc1 is highly expressed in post-embryonic stages. TcMhc2 shows peak expression in late pupae. TcMyo9 transcripts reached their highest levels in late pupae. TcMyo15, TcMyo7B and TcMhc2 are abundantly expressed in the adult epidermis, gut and testis, respectively. TcMyo9 and TcMyo20 are highly expressed in the epidermis, fat body and ovary, and TcMhc1 exhibits high mRNA levels in the epidermis and accessory gland. TcMyo20 RNAi reduced wing and leg size, fertility and egg hatchability. TcMyo9 knockdown completely inhibited eclosion and fecundity, but TcMyo15 or TcMhc1 silencing only impaired eclosion. TcMhc2 RNAi affected pupation and wing development. This study suggests myosin genes as potential targets of pesticides due to their critical roles in insect development and reproduction.

Identifiants

DOI: 10.1016/j.ygeno.2018.04.010 PMID: 29674165
pubmed: 29674165
pii: S0888-7543(18)30238-6
doi: 10.1016/j.ygeno.2018.04.010
pii:
doi:

Substances chimiques

Insect Proteins 0
RNA, Messenger 0
Myosin Heavy Chains EC 3.6.4.1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

719-728

Informations de copyright

Copyright © 2018 Elsevier Inc. All rights reserved.

Auteurs

Chengjun Li (C)

Institute of Life Sciences, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.

Juanjuan Liu (J)

Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.

Peng Lü (P)

Institute of Life Sciences, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: penglu@ujs.edu.cn.

Shangshang Ma (S)

Institute of Life Sciences, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.

Keming Zhu (K)

Institute of Life Sciences, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: uegzkg@sina.com.cn.

Lu Gao (L)

Institute of Life Sciences, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.

Bin Li (B)

Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China. Electronic address: libin@njnu.edu.cn.

Keping Chen (K)

Institute of Life Sciences, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: kpchen@ujs.edu.cn.

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Classifications MeSH

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