HPLC-DAD identification of polyphenols from ethyl acetate extract of Amaranthus spinosus leaves and determination of their antioxidant and antinociceptive effects.
Amaranthus spinosus
Antioxidant activity, antinociceptive
Bioactive compounds
Pain
Journal
Inflammopharmacology
ISSN: 1568-5608
Titre abrégé: Inflammopharmacology
Pays: Switzerland
ID NLM: 9112626
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
18
01
2018
accepted:
08
04
2018
pubmed:
22
4
2018
medline:
28
11
2019
entrez:
22
4
2018
Statut:
ppublish
Résumé
Amaranthus spinosus has been consumed traditionally to prevent various diseases including abdominal pain. In this study, the phytochemical composition, antioxidant and analgesic activities of an ethyl acetate extract of A. spinosus leaves (ASEA) were evaluated. The ASEA had the highest concentrations of total phenols (462.2 mg GAE/g DW), condensed tannin (5.01 mg CE/g DW) and total flavonoid contents (30.07 mg CE/g DW) compared to the chloroform, n-hexane, n-butanol and water extracts. Similarly, ASEA showed the most effective total antioxidant activity (45.45 µg/mL), DPPH scavenging activity (27.32 µg/mL) and hydrogen peroxide scavenging activity (30.60 µg/mL). ASEA with the doses of 200-600 mg/kg (p.o.) clearly demonstrated antinociceptive effects by reducing acetic acid-induced abdominal contortions with a maximal inhibition of 79.57% at 600 mg/kg and increasing latencies of the hot-plate paw-licking response. The tested doses also significantly (p < 0.001) decreased the reaction time in the formalin test at the neurogenic and inflammatory phases. ASEA contained ten polyphenols with caffeic acid being the predominant polyphenol. Overall, this study gave evidence that A. spinosus is a new antioxidant and analgesic agent, and justified its traditional use for the treatment of pain.
Identifiants
pubmed: 29679184
doi: 10.1007/s10787-018-0482-0
pii: 10.1007/s10787-018-0482-0
doi:
Substances chimiques
Acetates
0
Analgesics
0
Antioxidants
0
Flavonoids
0
Phenols
0
Plant Extracts
0
Polyphenols
0
ethyl acetate
76845O8NMZ
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
975-984Références
Amin I, Norazaidah Y, Emmy Hainida KI (2006) Antioxidant activity and phenolic content of raw and blanched Amaranthus species. Food Chem 94:47–52
doi: 10.1016/j.foodchem.2004.10.048
Bounatirou S, Smiti S, Miguel MG, Faleiro L, Rejeb MN, Neffati M, Pedro LG (2007) Chemical composition antioxidant and antibacterial activities of the essential oils isolated from Tunisian Thymus capitatus Hoff. et Link. Food Chem 105:146–155
doi: 10.1016/j.foodchem.2007.03.059
Carocho M, Ferreira IC (2013) A review on antioxidants prooxidants and related controversy natural and synthetic compounds screening and analysis methodologies and future perspectives. Food Chem Toxicol 51:15–25
doi: 10.1016/j.fct.2012.09.021
Coderre TJ, Vaccarino AL, Melzack R (1990) Central nervous system plasticity in the tonic pain response to subcutaneous formalin injection. Brain Res 535:155–158
doi: 10.1016/0006-8993(90)91835-5
Collier HOJ, Dinneen LC, Johnson CA, Schneider C (1968) The abdominal constriction response and its suppression by analgesic drugs in the mouse. Br J Pharmacol 32:295–310
Dewanto V, Wu X, Adom K, Liu RH (2002) Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 50:3010–3014
doi: 10.1021/jf0115589
Hlila MB, Mosbah H, Mssada K, Jannet HB, Aouni M, Selmi B (2015) Acetylcholinesterase inhibitory and antioxidant properties of roots extracts from the Tunisian Scabiosa arenaria Forssk. Ind Crop Prod 67:62–69
doi: 10.1016/j.indcrop.2015.01.009
Huang B, Ke H, He J, Ban X, Zeng H, Wang Y (2011) Extracts of Halenia elliptica exhibit antioxidant properties in vitro and in vivo. Food Chem Toxicol 49:185–190
doi: 10.1016/j.fct.2010.10.015
Hunskaar S, Hole K (1987) The formalin test in mice: dissociation between inflammatory and non-inflammatory pain. Pain 30:103–114
doi: 10.1016/0304-3959(87)90088-1
Ibrahim B, Sowemimo A, van Rooyen A, Van de Venter M (2012) Antiinflammatory analgesic and antioxidant activities of Cyathula prostrata (Linn.) Blume (Amaranthaceae). J Ethnopharmacol 141:282–289
doi: 10.1016/j.jep.2012.02.032
Ishtiaq S, Ali T, Ahmad B, Anwar F, Afridi MSK, Shaheen H (2017) Phytochemical and biological evaluations of methanolic extract of Amaranthus graecizans subsp. silvestris (Vill.) Brenan. Br J Pharm Res 15:1–11
doi: 10.9734/BJPR/2017/31698
Jayaprakasam B, Zhang Y, Nair MG (2004) Tumor cell proliferation and cyclooxygenase enzyme inhibitory compounds in Amaranthus tricolor. J Agric Food Chem 52:6939–6943
doi: 10.1021/jf048836z
Jayaprakasam B, Vanisree M, Zhang Y, Dewitt DL, Nair MG (2006) Impact of alkyl esters of caffeic and ferulic acids on tumor cell proliferation, cyclooxygenase enzyme, and lipid peroxidation. J Agric Food Chem 54:5375–5381
doi: 10.1021/jf060899p
Joana Gil-Chávez G, Villa JA, Fernando Ayala-Zavala J, Basilio Heredia J, Sepulveda D, Yahia EM, González-Aguilar GA (2013) Technologies for extraction and production of bioactive compounds to be used as nutraceuticals and food ingredients an overview. Compreh Rev Food Sci Food Saf 12:5–23
doi: 10.1111/1541-4337.12005
Khoudja NK, Boulekbache-Makhlouf L, Madani K (2014) Antioxidant capacity of crude extracts and their solvent fractions of selected Algerian Lamiaceae. Ind Crop Prod 52:177–182
doi: 10.1016/j.indcrop.2013.10.004
Kim HP, Son KH, Chang HW, Kang SS (2004) Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacol Sci 96:229–245
doi: 10.1254/jphs.CRJ04003X
Kumar BSA, Lakshman K, Jayaveera KN, Shekar DS, Nandeesh R, Velmurugan C (2010) Chemoprotective and antioxidant activities of methanolic extract of Amaranthus spinosus leaves on paracetamol induced-liver damage in rats. Acta Med Salin 39:68–74
Liu J, Luo J, Ye H, Sun Y, Lu Z, Zeng X (2010) In vitro and in vivo antioxidant activity of exopolysaccharides from endophytic bacterium Paenibacillus polymyxa EJS-3. Carbohydr Polym 82:1278–1283
doi: 10.1016/j.carbpol.2010.07.008
Mariod AA, Ibrahim RM, Ismail M, Ismail N (2009) Antioxidant activity and phenolic content of phenolic rich fractions obtained from black cumin (Nigella sativa) seedcake. Food Chem 116:306–312
doi: 10.1016/j.foodchem.2009.02.051
Mehrotra A, Shanbhag R, Chamallamudi MR, Singh VP, Mudgal J (2011) Ameliorative effect of caffeic acid against inflammatory pain in rodents. Eur J Pharmacol 666:80–86
doi: 10.1016/j.ejphar.2011.05.039
Miller T (1983) Protective effects of prostaglandins against gastric mucosal damage: current knowledge and proposed mechanisms. Ame J Physiol Gastrointest Liver Physiol 245:G601–G623
doi: 10.1152/ajpgi.1983.245.5.G601
Naczk M, Shahidi F (2006) Phenolics in cereals fruits and vegetables occurrence extraction and analysis. J Pharm Biomed Anal 41:1523–1542
doi: 10.1016/j.jpba.2006.04.002
Nardini M, Leonardi F, Scaccini C, Virgili F (2001) Modulation of ceramide-induced NF-κB binding activity and apoptotic response by caffeic acid in U937 cells: comparison with other antioxidants. Free Rad Biol Med 30:722–733
doi: 10.1016/S0891-5849(00)00515-3
Nwidu LL, Nwafor PA, Da Silva VC, Rodrigues CM, dos Santos LC, Vilegas W, Nunes-de-Souza RL (2011) Anti-nociceptive effects of Carpolobia lutea G. Don (Polygalaceae) leaf fractions in animal models. Inflammopharmacol 19:215–225
doi: 10.1007/s10787-010-0076-y
Okoli CO, Akah PA, Nwafor SV, Anisiobi AI, Ibegbunam IN, Erojikwe O (2007) Anti-inflammatory activity of hexane leaf extract of Aspilia africana CD Adams. J Ethnopharmacol 109:219–225
doi: 10.1016/j.jep.2006.07.037
Prasad KN, Yang B, Yang S, Chen Y, Zhao M, Ashraf M, Jiang Y (2009) Identification of phenolic compounds and appraisal of antioxidant and antityrosinase activities from litchi (Litchi sinensis Sonn.) seeds. Food Chem 116:1–7
doi: 10.1016/j.foodchem.2009.01.079
Rjeibi I, Saad AB, Hfaiedh N (2016) Oxidative damage and hepatotoxicity associated with deltamethrin in rats: the protective effects of Amaranthus spinosus seed extract. Biomed Pharmacother 84:853–860
doi: 10.1016/j.biopha.2016.10.010
Schmauss C, Yaksh TL (1984) In vivo studies on spinal opiate receptor systems mediating antinociception. II. Pharmacological profiles suggesting a differential association of mu delta and kappa receptors with visceral chemical and cutaneous thermal stimuli in the rat. J Pharmacol Exp Ther 228:1–12
pubmed: 6319664
Sun B, Ricardo-da-Silva JM, Spranger I (1998) Critical factors of vanillin assay for catechins and proanthocyanidins. J Agric Food Chem 46:4267–4274
doi: 10.1021/jf980366j
Tanmoy G, Arijit M, Tanushree S, Jagadish S, Kumar MT (2014) Pharmacological actions and phytoconstituents of Amaranthus spinosus Linn a review. Intern J Pharmacogn Phytochem Res 6:405–413
Tlili N, Elfalleh W, Hannachi H, Yahia Y, Khaldi A, Ferchichi A, Nasri N (2013) Screening of natural antioxidants from selected medicinal plants. Int J Food Prop 16:1117–1126
doi: 10.1080/10942912.2011.576360
Vaz ZR, Mata LV, Calixto JB (1997) Analgesic effect of the herbal medicine catuama in thermal and chemical models of nociception in mice. Phytother Res 11:101–110
doi: 10.1002/(SICI)1099-1573(199703)11:2<101::AID-PTR28>3.0.CO;2-U
Wheeler-Aceto H, Porreca F, Cowan A (1990) The rat paw formalin test: comparison of noxious agents. Pain 40:229–238
doi: 10.1016/0304-3959(90)90073-M
Xu Y, Lin D, Yu X, Xie X, Wang L, Lian L, Huang X (2016) The antinociceptive effects of ferulic acid on neuropathic pain: involvement of descending monoaminergic system and opioid receptors. Oncotarget 7:20455–20468
pubmed: 26967251
pmcid: 4991467
Ye Küpeli E, Silada E (2007) Flavonoids with anti-inflammatory and antinociceptive activity from Cistus laurifolius L. leaves through bioassay-guided procedures. J Ethnopharmacol 112:524–530
doi: 10.1016/j.jep.2007.04.011
Ymele EV, Dongmo AB, Dimo T (2013) Analgesic and anti-inflammatory effect of aqueous extract of the stem bark of Allanblackia gabonensis (Guttiferae). Inflammopharmacol 21:21–30
doi: 10.1007/s10787-011-0096-2
Zeashan H, Amresh G, Singh S, Rao CV (2008) Hepatoprotective activity of Amaranthus spinosus in experimental animals. Food Chem Toxicol 46:3417–3421
doi: 10.1016/j.fct.2008.08.013
Zeashan H, Amresh G, Rao CV, Singh S (2009) Antinociceptive activity of Amaranthus spinosus in experimental animals. J Ethnopharmacol 122:492–496
doi: 10.1016/j.jep.2009.01.031