Optimised detection of mitochondrial DNA strand breaks.

Adult DNA Breaks DNA, Mitochondrial / analysis Epithelial Cells / drug effects Humans Hydrogen Peroxide / toxicity Male Real-Time Polymerase Chain Reaction / methods
Biomarker Human Long range qPCR Mitochondrial DNA damage Oxidative stress Skin

Journal

Mitochondrion
ISSN: 1872-8278
Titre abrégé: Mitochondrion
Pays: Netherlands
ID NLM: 100968751

Informations de publication

Date de publication:
05 2019
Historique:
received: 21 12 2017
revised: 09 04 2018
accepted: 30 04 2018
pubmed: 8 5 2018
medline: 14 8 2019
entrez: 7 5 2018
Statut: ppublish

Résumé

Intrinsic and extrinsic factors that induce cellular oxidative stress damage tissue integrity and promote ageing, resulting in accumulative strand breaks to the mitochondrial DNA (mtDNA) genome. Limited repair mechanisms and close proximity to superoxide generation make mtDNA a prominent biomarker of oxidative damage. Using human DNA we describe an optimised long-range qPCR methodology that sensitively detects mtDNA strand breaks relative to a suite of short mitochondrial and nuclear DNA housekeeping amplicons, which control for any variation in mtDNA copy number. An application is demonstrated by detecting 16-36-fold mtDNA damage in human skin cells induced by hydrogen peroxide and solar simulated radiation.

Identifiants

DOI: 10.1016/j.mito.2018.04.009 PMID: 29730480
pubmed: 29730480
pii: S1567-7249(17)30345-8
doi: 10.1016/j.mito.2018.04.009
pii:
doi:

Substances chimiques

DNA, Mitochondrial 0
Hydrogen Peroxide BBX060AN9V

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Pagination

172-178

Subventions

Organisme : Biotechnology and Biological Sciences Research Council
ID : BH151766
Pays : United Kingdom

Informations de copyright

Copyright © 2018 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

Auteurs

Rebecca Hanna (R)

Dermatological Sciences, Institute of Cellular Medicine, The Medical School, Newcastle University, Newcastle NE24HH, UK.

Jonathan M Crowther (JM)

JMC Scientific Consulting Ltd., Egham, Surrey, TW208LL, UK.

Pallav A Bulsara (PA)

GSK Consumer Healthcare, 184 Liberty Corner Rd, Warren, NJ 07059, USA.

Xuying Wang (X)

GSK Consumer Healthcare, 1250 S. Collegeville Road, Collegeville, PA 19426-0989, USA.

David J Moore (DJ)

GSK Consumer Healthcare, 184 Liberty Corner Rd, Warren, NJ 07059, USA.

Mark A Birch-Machin (MA)

Dermatological Sciences, Institute of Cellular Medicine, The Medical School, Newcastle University, Newcastle NE24HH, UK. Electronic address: mark.birch-machin@ncl.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Optimised detection of mitochondrial DNA strand breaks.
questionsmedicales.fr › Phénomènes génétiques › Altération de l'ADN › Cassures de l'ADN › Optimised detection of mitochondrial DNA strand breaks.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN mitochondrial › Optimised detection of mitochondrial DNA strand breaks.
questionsmedicales.fr › Cellules › Cellules épithéliales › Optimised detection of mitochondrial DNA strand breaks.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Optimised detection of mitochondrial DNA strand breaks.
questionsmedicales.fr › Composés chimiques organiques › Radicaux libres › Peroxydes › Peroxyde d'hydrogène › Optimised detection of mitochondrial DNA strand breaks.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › Optimised detection of mitochondrial DNA strand breaks.