Functionalized Magnetic Bacterial Cellulose Beads as Carrier for Lecitase® Ultra Immobilization.
Bacterial cellulose
Immobilization
Lecitase® Ultra
Modification
Journal
Applied biochemistry and biotechnology
ISSN: 1559-0291
Titre abrégé: Appl Biochem Biotechnol
Pays: United States
ID NLM: 8208561
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
12
03
2018
accepted:
07
06
2018
pubmed:
19
6
2018
medline:
13
2
2019
entrez:
19
6
2018
Statut:
ppublish
Résumé
Bacterial cellulose spheres subjected to amination and inlaid modification with superparamagnetic molecules were analyzed with regard to possibility of their application as an immobilization carrier of Lecitase® Ultra (LU) enzyme. The starting point to obtain the carrier was synthesis of bacterial cellulose spheres performed in shaking cultures of Komagataeibacter xylinus. These spheres were subsequently subjected to a multi-stage modification to increase the efficiency of the immobilization process and to separate product from the reaction medium. Maximal yield of Lecitase® Ultra immobilization equaled 70%. It was also found that immobilization process did not affect the pH and LU temperature optimum. Moreover, immobilized enzyme exhibited similar temperature stability profile as its native form. The immobilization process did not significantly affect the enzyme K
Identifiants
pubmed: 29911267
doi: 10.1007/s12010-018-2816-1
pii: 10.1007/s12010-018-2816-1
pmc: PMC6326999
doi:
Substances chimiques
Enzymes, Immobilized
0
Cellulose
9004-34-6
Lipase
EC 3.1.1.3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
176-193Subventions
Organisme : National Centre for Research and Development in Poland
ID : LIDER/011/221/L-5/13/NCBR/2014
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