Copper stress response in yeast Rhodotorula mucilaginosa AN5 isolated from sea ice, Antarctic.
Antarctic Regions
Antioxidants
/ metabolism
Copper
/ toxicity
Drug Resistance, Fungal
Ice Cover
/ microbiology
Microscopy, Electron, Scanning
Oxidation-Reduction
Proteome
/ analysis
Rhodotorula
/ chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Stress, Physiological
Surface Properties
Trace Elements
/ toxicity
Antarctica yeast
adaptive responses
copper stress
proteomics
Journal
MicrobiologyOpen
ISSN: 2045-8827
Titre abrégé: Microbiologyopen
Pays: England
ID NLM: 101588314
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
received:
26
12
2017
revised:
24
03
2018
accepted:
26
04
2018
pubmed:
22
6
2018
medline:
18
5
2019
entrez:
22
6
2018
Statut:
ppublish
Résumé
Heavy metal pollution in Antarctic is serious by anthropogenic emissions and atmospheric transport. To dissect the heavy metal adaptation mechanisms of sea-ice organisms, a basidiomycetous yeast strain AN5 was isolated and its cellular changes were analyzed. Morphological, physiological, and biochemical characterization indicated that this yeast strain belonged to Rhodotorula mucilaginosa AN5. Heavy metal resistance pattern of Cd > Pb = Mn > Cu > Cr > Hg was observed. Scanning electron microscopic (SEM) results exhibited altered cell surface morphology under the influence of copper metal compared to that with control. The determination of physiological and biochemical changes manifested that progressive copper treatment significantly increased antioxidative reagents content and enzymes activity in the red yeast, which quench the active oxygen species to maintain the intercellular balance of redox state and ensure the cellular fission and growth. Comparative proteomic analysis revealed that, under 2 mM copper stress, 95 protein spots were tested reproducible changes of at least 10-fold in cells. Among 95 protein spots, 43 were elevated and 52 were decreased synthesis. After MALDI TOF MS/MS analysis, 51 differentially expressed proteins were identified successfully and classified into six functional groups, including carbohydrate and energy metabolism, nucleotide and protein metabolism, protein folding, antioxidant system, signaling, and unknown function proteins. Function analysis indicated that carbohydrate and energy metabolism-, nucleotide and protein metabolism-, and protein folding-related proteins played central role to the heavy metal resistance of Antarctic yeast. Generally, the results revealed that the yeast has a great capability to cope with heavy metal stress and activate the physiological and protein mechanisms, which allow more efficient recovery after copper stress. Our studies increase understanding of the molecular resistance mechanism of polar yeast to heavy metal, which will be benefitted for the sea-ice isolates to be a potential candidate for bioremediation of metal-contaminated environments.
Identifiants
pubmed: 29926536
doi: 10.1002/mbo3.657
pmc: PMC6436437
doi:
Substances chimiques
Antioxidants
0
Proteome
0
Trace Elements
0
Copper
789U1901C5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e00657Informations de copyright
© 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
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