Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport.
Actin Cytoskeleton
/ metabolism
Animals
CRISPR-Cas Systems
Cells, Cultured
Intermediate Filaments
/ metabolism
Keratin-18
/ metabolism
Keratin-8
/ metabolism
Kinesins
/ antagonists & inhibitors
Mice
Mice, Knockout
Microscopy, Fluorescence
Microtubules
/ metabolism
Retinal Pigment Epithelium
/ metabolism
Vimentin
/ metabolism
KIF5B
photoconversion
vimentin
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
pubmed:
27
6
2018
medline:
10
7
2019
entrez:
27
6
2018
Statut:
ppublish
Résumé
Keratin intermediate filaments (IFs) are the major cytoskeletal component in epithelial cells. The dynamics of keratin IFs have been described to depend mostly on the actin cytoskeleton, but the rapid transport of fully polymerized keratin filaments has not been reported. In this work, we used a combination of photoconversion experiments and clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeats-associated protein 9 genome editing to study the role of microtubules and microtubule motors in keratin filament transport. We found that long keratin filaments, like other types of IFs, are transported along microtubules by kinesin-1. Our data revealed that keratin and vimentin are nonconventional kinesin-1 cargoes because their transport did not require kinesin light chains, which are a typical adapter for kinesin-dependent cargo transport. Furthermore, we found that the same domain of the kinesin heavy chain tail is involved in keratin and vimentin IF transport, strongly suggesting that multiple types of IFs move along microtubules using an identical mechanism.-Robert, A., Tian, P., Adam, S. A., Kittisopikul, M., Jaqaman, K., Goldman, R. D., Gelfand, V. I. Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport.
Identifiants
pubmed: 29944446
doi: 10.1096/fj.201800604R
pmc: PMC6355078
doi:
Substances chimiques
Keratin-18
0
Keratin-8
0
Krt8 protein, mouse
0
Vim protein, mouse
0
Vimentin
0
Kif5b protein, mouse
EC 3.6.1.-
Kinesins
EC 3.6.4.4
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
388-399Subventions
Organisme : NIGMS NIH HHS
ID : P01 GM096971
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM052111
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM119619
Pays : United States
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