Effect of extracorporeal low-energy shock wave on diabetic gastroparesis in a rat model.
Animals
Axons
/ physiology
Diabetes Complications
Diabetes Mellitus, Experimental
Disease Models, Animal
Extracorporeal Shockwave Therapy
Gastric Emptying
Gastrointestinal Motility
Gastroparesis
/ etiology
High-Energy Shock Waves
Male
Myenteric Plexus
/ pathology
Nerve Regeneration
Rats, Wistar
Stomach
/ innervation
Streptozocin
diabetes mellitus
gastric motility
gastroparesis
low-energy shock wave
rat model
Journal
Journal of gastroenterology and hepatology
ISSN: 1440-1746
Titre abrégé: J Gastroenterol Hepatol
Pays: Australia
ID NLM: 8607909
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
20
03
2018
revised:
20
06
2018
accepted:
20
06
2018
pubmed:
3
7
2018
medline:
10
7
2019
entrez:
3
7
2018
Statut:
ppublish
Résumé
Delayed gastric emptying occurs in more than 50% of chronic diabetic patients, and this is associated with significant impairments in quality of life. Traditional therapy for delayed gastric emptying has focused on supportive treatment, and there is no significant effective therapy. The effect of low-energy shock wave on gastric motility is never studied. We investigated low-energy shock wave on gastric motility in a diabetic rat model. Twenty-eight male Wistar rats were studied and separated in three groups in randomized order as control, diabetic rats received shock wave, and diabetic rats received the sham procedure. Antral area and motility were recorded using the transabdominal ultrasound. Blood was taken for measurement of gastric motility peptides. Subjects were killed for immunohistochemical stain analysis of enteric plexus of the stomach. We successfully induced 20 diabetic rats and set ultrasound for measuring rat gastric contract and emptying model and demonstrated that 6 weeks of low-energy shock wave could promote gastric contraction and emptying in diabetic rats. Moreover, we demonstrated that shock wave could increase defecation and feces and decrease serum cholesterol and triglycerol. However, no effect on glycohemoglobin and gastric motility peptides was recorded. In the immunohistochemical staining, shock wave increased expression of gastric myenteric neuron plexus. Low-energy shock wave can increase gastric contraction and emptying by activating axonal regeneration and increasing myenteric plexus, but not related with motility peptides.
Sections du résumé
BACKGROUND AND AIM
OBJECTIVE
Delayed gastric emptying occurs in more than 50% of chronic diabetic patients, and this is associated with significant impairments in quality of life. Traditional therapy for delayed gastric emptying has focused on supportive treatment, and there is no significant effective therapy. The effect of low-energy shock wave on gastric motility is never studied. We investigated low-energy shock wave on gastric motility in a diabetic rat model.
METHODS
METHODS
Twenty-eight male Wistar rats were studied and separated in three groups in randomized order as control, diabetic rats received shock wave, and diabetic rats received the sham procedure. Antral area and motility were recorded using the transabdominal ultrasound. Blood was taken for measurement of gastric motility peptides. Subjects were killed for immunohistochemical stain analysis of enteric plexus of the stomach.
RESULTS
RESULTS
We successfully induced 20 diabetic rats and set ultrasound for measuring rat gastric contract and emptying model and demonstrated that 6 weeks of low-energy shock wave could promote gastric contraction and emptying in diabetic rats. Moreover, we demonstrated that shock wave could increase defecation and feces and decrease serum cholesterol and triglycerol. However, no effect on glycohemoglobin and gastric motility peptides was recorded. In the immunohistochemical staining, shock wave increased expression of gastric myenteric neuron plexus.
CONCLUSION
CONCLUSIONS
Low-energy shock wave can increase gastric contraction and emptying by activating axonal regeneration and increasing myenteric plexus, but not related with motility peptides.
Substances chimiques
Streptozocin
5W494URQ81
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
720-727Subventions
Organisme : Chang Gung Memorial Hospital- Kaohsiung
ID : CMRPG8F0491
Informations de copyright
© 2018 Journal of Gastroenterology and Hepatology Foundation and John Wiley & Sons Australia, Ltd.