Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae).
Animals
Arvicolinae
/ parasitology
Base Sequence
Chickens
Cryptosporidiosis
/ epidemiology
Cryptosporidium
/ classification
Czech Republic
DNA, Protozoan
/ chemistry
Feces
/ parasitology
Female
Gastrointestinal Tract
/ parasitology
Genetic Variation
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Microscopy, Electron, Scanning
Microscopy, Fluorescence
Microscopy, Interference
Murinae
Phylogeny
Polymerase Chain Reaction
Prevalence
RNA, Ribosomal
/ genetics
Rats
Rodent Diseases
/ epidemiology
Sequence Alignment
/ veterinary
Experimental infection
Rodentia
molecular analyses
oocyst size
phylogeny
voles
Journal
Parasitology
ISSN: 1469-8161
Titre abrégé: Parasitology
Pays: England
ID NLM: 0401121
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
pubmed:
18
7
2018
medline:
8
1
2020
entrez:
18
7
2018
Statut:
ppublish
Résumé
Fecal samples from wild-caught common voles (n = 328) from 16 locations in the Czech Republic were screened for Cryptosporidium by microscopy and PCR/sequencing at loci coding small-subunit rRNA, Cryptosporidium oocyst wall protein, actin and 70 kDa heat shock protein. Cryptosporidium infections were detected in 74 voles (22.6%). Rates of infection did not differ between males and females nor between juveniles and adults. Phylogenetic analysis revealed the presence of eight Cryptosporidium species/genotypes including two new species, C. alticolis and C. microti. These species from wild-caught common voles were able to infect common and meadow voles under experimental conditions, with a prepatent period of 3-5 days post-infection (DPI), but they were not infectious for various other rodents or chickens. Meadow voles lost infection earlier than common voles (11-14 vs 13-16 DPI) and had significantly lower infection intensity. Cryptosporidium alticolis infects the anterior small intestine and has larger oocysts (5.4 × 4.9 µm), whereas C. microti infects the large intestine and has smaller oocysts (4.3 × 4.1 µm). None of the rodents developed clinical signs of infection. Genetic and biological data support the establishment of C. alticolis and C. microti as separate species of the genus Cryptosporidium.
Identifiants
pubmed: 30012231
pii: S0031182018001142
doi: 10.1017/S0031182018001142
pmc: PMC6994189
mid: NIHMS1063511
doi:
Substances chimiques
DNA, Protozoan
0
RNA, Ribosomal
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
220-233Subventions
Organisme : NIAID NIH HHS
ID : R15 AI122152
Pays : United States
Références
Eur J Protistol. 2018 Apr;63:1-12
pubmed: 29360041
Appl Environ Microbiol. 2005 Aug;71(8):4446-54
pubmed: 16085835
Lancet Infect Dis. 2015 Jan;15(1):85-94
pubmed: 25278220
Folia Parasitol (Praha). 1998;45(2):173-4
pubmed: 9684328
J Clin Microbiol. 2013 Jan;51(1):360-2
pubmed: 23100342
Vet Parasitol. 2015 Sep 15;212(3-4):404-7
pubmed: 26186985
J Parasitol. 2004 Aug;90(4):769-73
pubmed: 15357067
Vet Parasitol. 2009 Dec 3;166(1-2):32-7
pubmed: 19713046
Appl Environ Microbiol. 2007 Dec;73(23):7693-6
pubmed: 17921267
Vet Parasitol. 2007 Feb 28;143(3-4):229-33
pubmed: 16997473
Ann Agric Environ Med. 2015;22(1):1-5
pubmed: 25780818
Appl Environ Microbiol. 2009 Dec;75(24):7692-9
pubmed: 19820152
Infect Genet Evol. 2015 Dec;36:287-293
pubmed: 26437239
Vet Parasitol. 2014 May 28;202(3-4):113-8
pubmed: 24768316
Exp Parasitol. 2010 Jan;124(1):90-7
pubmed: 19303009
J Wildl Dis. 2007 Oct;43(4):586-96
pubmed: 17984253
Vet Parasitol. 2004 Jul 14;122(3):183-92
pubmed: 15219359
J Wildl Dis. 1994 Jan;30(1):110-1
pubmed: 8151812
Vet Parasitol. 2011 Mar 10;176(2-3):120-5
pubmed: 21131129
Folia Parasitol (Praha). 2008 Jun;55(2):81-94
pubmed: 18666410
Vet Parasitol. 1999 Jan 28;80(4):273-80
pubmed: 9950333
Exp Parasitol. 2010 Jan;124(1):128-37
pubmed: 19084007
Folia Parasitol (Praha). 2008 Jun;55(2):95-9
pubmed: 18666411
Parasitol Res. 2012 Jul;111(1):471-3
pubmed: 22302477
J Parasitol. 2002 Apr;88(2):388-94
pubmed: 12054017
FEMS Microbiol Lett. 1997 May 15;150(2):209-17
pubmed: 9170264
J Vet Med B Infect Dis Vet Public Health. 2006 Jun;53(5):239-43
pubmed: 16732883
Emerg Infect Dis. 2014 Feb;20(2):217-24
pubmed: 24447504
Vet Parasitol. 2011 Jun 30;179(1-3):216-9
pubmed: 21429669
Clin Microbiol Rev. 2004 Jan;17(1):72-97
pubmed: 14726456
Appl Environ Microbiol. 1999 Apr;65(4):1578-83
pubmed: 10103253
Parasitol Res. 2007 Feb;100(3):455-60
pubmed: 17004098
Infect Genet Evol. 2013 Jun;16:5-12
pubmed: 23376410
Appl Environ Microbiol. 2004 Dec;70(12):7574-7
pubmed: 15574965
Appl Environ Microbiol. 2007 Oct;73(20):6475-83
pubmed: 17720824
Appl Environ Microbiol. 2001 Mar;67(3):1154-62
pubmed: 11229905
Parasitology. 2002 Jul;125(Pt 1):21-34
pubmed: 12166517
Mol Biol Evol. 2013 Dec;30(12):2725-9
pubmed: 24132122
Syst Biol. 2003 Oct;52(5):696-704
pubmed: 14530136
Parasitol Res. 2005 Nov;97(5):373-9
pubmed: 16151744
Parasitol Res. 1997;83(5):478-82
pubmed: 9197396
Vet Parasitol. 2007 Jun 20;147(1-2):176-84
pubmed: 17466459
Parasitology. 2018 Mar;145(3):326-334
pubmed: 28870264
Vet Parasitol. 2000 Oct 20;92(4):253-60
pubmed: 10996736
Parasitol Res. 2008 Dec;104(1):1-17
pubmed: 18791739
Int J Parasitol. 2013 Sep;43(10):805-17
pubmed: 23791796
Appl Environ Microbiol. 2000 Jun;66(6):2385-91
pubmed: 10831415
Vet Parasitol. 2012 Mar 23;184(2-4):122-5
pubmed: 21917378
J Comp Pathol. 2016 Jul;155(1 Suppl 1):S54-74
pubmed: 26687277
Eur J Protistol. 2018 Apr;63:96-104
pubmed: 29506004
J Eukaryot Microbiol. 1996 Sep-Oct;43(5):89S
pubmed: 8822880
Folia Parasitol (Praha). 1985;32(1):50
pubmed: 2580763
J Parasitol. 2003 Oct;89(5):1053-5
pubmed: 14627156
Infect Genet Evol. 2015 Jun;32:113-23
pubmed: 25772204
Acta Trop. 2017 Aug;172:29-35
pubmed: 28433573