Mitochondrial Redox Sensor for Drosophila Female Germline Stem Cells.
Animals
Animals, Genetically Modified
/ genetics
Biosensing Techniques
Cell Communication
Drosophila Proteins
/ genetics
Drosophila melanogaster
/ genetics
Female
Genes, Reporter
Glutaredoxins
/ genetics
Green Fluorescent Proteins
/ genetics
Mitochondria
/ genetics
Mitophagy
Oogonial Stem Cells
/ metabolism
Oxidation-Reduction
Promoter Regions, Genetic
RNA-Binding Proteins
/ genetics
Reactive Oxygen Species
/ analysis
Recombinant Fusion Proteins
/ metabolism
Drosophila
Germline stem cells
Mito-roGFP2-Grx1
Mitophagy
Redox
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
pubmed:
22
7
2018
medline:
5
6
2019
entrez:
21
7
2018
Statut:
ppublish
Résumé
Mitochondrial reactive oxygen species (mROS), a major source of ROS within cells, functions as an important signaling molecule and has the ability to damage cellular macromolecules including DNA and proteins. Monitoring mROS levels is therefore essential to understand cell-cell communication and programmed cell death in all types of cell including stem cells. Here, we describe generation and characterization of a redox sensor for mROS that is specifically expressed in the germline stem cells (GSCs) in Drosophila. This redox sensor can be used to monitor the production of mROS and mitophagy in the GSCs during oogenesis.
Identifiants
pubmed: 30027507
doi: 10.1007/7651_2018_167
doi:
Substances chimiques
Drosophila Proteins
0
Glutaredoxins
0
RNA-Binding Proteins
0
Reactive Oxygen Species
0
Recombinant Fusion Proteins
0
nos protein, Drosophila
142661-95-8
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM