Correlative iPALM and SEM resolves virus cavity and Gag lattice defects in HIV virions.
CLEM
Gag
HIV
SEM
iPALM
Journal
European biophysics journal : EBJ
ISSN: 1432-1017
Titre abrégé: Eur Biophys J
Pays: Germany
ID NLM: 8409413
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
18
12
2017
accepted:
16
07
2018
revised:
27
03
2018
pubmed:
26
7
2018
medline:
5
2
2019
entrez:
26
7
2018
Statut:
ppublish
Résumé
Interferometric Photo-Activation-Localization-Microscopy (iPALM) localizes single fluorescent molecules with 20 nm lateral and 10 nm axial resolution. We present a method utilizing glass coverslip lithography for correlative imaging between iPALM and scanning electron microscopy (SEM). Using iPALM on HIV Gag-Dendra virus-like particles (VLPs) we localized the position of HIV Gag proteins. Based on these localizations we reconstructed the central cavity of the VLPs along with imperfections within the HIV Gag lattice. The SEM images and iPALM images overlap and show imaging from single VLPs immobilized on glass coverslips. The localization of many HIV proteins including accessory proteins and Gag-Pol remains unknown, we discuss how the specificity of iPALM coupled with SEM has the potential for resolving more of HIV proteins.
Identifiants
pubmed: 30043246
doi: 10.1007/s00249-018-1324-0
pii: 10.1007/s00249-018-1324-0
pmc: PMC6330563
doi:
Substances chimiques
gag Gene Products, Human Immunodeficiency Virus
0
Gold
7440-57-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
15-23Subventions
Organisme : Directorate for Biological Sciences
ID : 1615076
Références
Biophys J. 2002 May;82(5):2775-83
pubmed: 11964263
Tsitologiia. 2003;45(1):86-93
pubmed: 12683241
Nat Struct Mol Biol. 2004 Jul;11(7):672-5
pubmed: 15208690
Nat Methods. 2006 Oct;3(10):793-5
pubmed: 16896339
Biophys J. 2006 Dec 1;91(11):4258-72
pubmed: 16980368
Dev Cell. 2006 Oct;11(4):438-9
pubmed: 17011484
Science. 2008 Feb 8;319(5864):810-3
pubmed: 18174397
Nat Methods. 2008 Jun;5(6):527-9
pubmed: 18469823
Nature. 2008 Jul 10;454(7201):236-40
pubmed: 18500329
Cell Host Microbe. 2008 Dec 11;4(6):592-9
pubmed: 19064259
Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3125-30
pubmed: 19202073
Biophys J. 2009 Mar 4;96(5):1961-9
pubmed: 19254556
Proc Natl Acad Sci U S A. 2009 Jul 7;106(27):11090-5
pubmed: 19549863
PLoS Pathog. 2009 Nov;5(11):e1000652
pubmed: 19893629
PLoS Pathog. 2010 Nov 24;6(11):e1001173
pubmed: 21124872
Nat Cell Biol. 2011 Apr;13(4):394-401
pubmed: 21394083
Nat Cell Biol. 2011 Apr;13(4):469-74
pubmed: 21394086
Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17436-41
pubmed: 23045631
Biochem Biophys Res Commun. 2013 Oct 18;440(2):271-6
pubmed: 24055706
Biophys J. 2013 Nov 19;105(10):2262-72
pubmed: 24268138
J Vis Exp. 2014 Jan 02;(83):e51366
pubmed: 24429535
Science. 2014 Feb 7;343(6171):653-6
pubmed: 24436186
PLoS One. 2014 May 16;9(5):e96950
pubmed: 24834918
Proc Natl Acad Sci U S A. 2014 Aug 19;111(33):12211-6
pubmed: 25099357
Nature. 2015 Jan 22;517(7535):505-8
pubmed: 25363765
Proc Natl Acad Sci U S A. 2015 Jan 13;112(2):E110-8
pubmed: 25535361
PLoS Pathog. 2015 Feb 24;11(2):e1004677
pubmed: 25710462
Cell. 1989 Oct 6;59(1):103-12
pubmed: 2676191
PLoS Pathog. 2016 Jun 09;12(6):e1005657
pubmed: 27280284
Nat Protoc. 2017 May;12(5):916-946
pubmed: 28384138
Biophys J. 1976 Nov;16(11):1315-29
pubmed: 974223