Protective role of soluble adenylyl cyclase against reperfusion-induced injury of cardiac cells.
Adenylyl Cyclases
/ metabolism
Animals
Cell Line
Cell Survival
Cyclic AMP
Cyclic Nucleotide Phosphodiesterases, Type 2
/ metabolism
Cytosol
/ metabolism
Disease Models, Animal
Hydrogen-Ion Concentration
Male
Mitochondria, Heart
/ metabolism
Myocytes, Cardiac
/ metabolism
Necrosis
Rats
Rats, Wistar
Reperfusion Injury
/ drug therapy
Signal Transduction
Cardiomyocytes
Mitochondrial cAMP
Phosphodiesterase 2
Reperfusion injury
Soluble adenylyl cyclase
Journal
Biochimica et biophysica acta. Molecular basis of disease
ISSN: 1879-260X
Titre abrégé: Biochim Biophys Acta Mol Basis Dis
Pays: Netherlands
ID NLM: 101731730
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
14
01
2018
revised:
15
06
2018
accepted:
17
07
2018
pubmed:
26
7
2018
medline:
14
8
2019
entrez:
26
7
2018
Statut:
ppublish
Résumé
Disturbance of mitochondrial function significantly contributes to the myocardial injury that occurs during reperfusion. Increasing evidence suggests a role of intra-mitochondrial cyclic AMP (cAMP) signaling in promoting respiration and ATP synthesis. Mitochondrial levels of cAMP are controlled by type 10 soluble adenylyl cyclase (sAC) and phosphodiesterase 2 (PDE2), however their role in the reperfusion-induced injury remains unknown. Here we aimed to examine whether sAC may support cardiomyocyte survival during reperfusion. Adult rat cardiomyocytes or rat cardiac H9C2 cells were subjected to metabolic inhibition and recovery as a model of simulated ischemia and reperfusion. Cytosolic Ca sAC plays an essential role in supporting cardiomyocytes viability during reperfusion. Elevation of mitochondrial cAMP pool either by sAC overexpression or by PDE2 inhibition beneficially affects cardiomyocyte survival during reperfusion.
Identifiants
pubmed: 30044950
pii: S0925-4439(18)30270-9
doi: 10.1016/j.bbadis.2018.07.021
pii:
doi:
Substances chimiques
Cyclic AMP
E0399OZS9N
Cyclic Nucleotide Phosphodiesterases, Type 2
EC 3.1.4.17
Adenylyl Cyclases
EC 4.6.1.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
252-260Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.