Effects of non-euphoric plant cannabinoids on muscle quality and performance of dystrophic mdx mice.


Journal

British journal of pharmacology
ISSN: 1476-5381
Titre abrégé: Br J Pharmacol
Pays: England
ID NLM: 7502536

Informations de publication

Date de publication:
05 2019
Historique:
received: 08 04 2018
revised: 08 07 2018
accepted: 12 07 2018
pubmed: 4 8 2018
medline: 22 7 2020
entrez: 4 8 2018
Statut: ppublish

Résumé

Duchenne muscular dystrophy (DMD), caused by dystrophin deficiency, results in chronic inflammation and irreversible skeletal muscle degeneration. Moreover, the associated impairment of autophagy greatly contributes to the aggravation of muscle damage. We explored the possibility of using non-euphoric compounds present in Cannabis sativa, cannabidiol (CBD), cannabidivarin (CBDV) and tetrahydrocannabidivarin (THCV), to reduce inflammation, restore functional autophagy and positively enhance muscle function in vivo. Using quantitative PCR, western blots and [Ca CBD and CBDV promoted the differentiation of murine C2C12 myoblast cells into myotubes by increasing [Ca We provide new insights into plant cannabinoid interactions with TRP channels in skeletal muscle, highlighting a potential opportunity for novel co-adjuvant therapies to prevent muscle degeneration in DMD patients. This article is part of a themed section on 8

Sections du résumé

BACKGROUND AND PURPOSE
Duchenne muscular dystrophy (DMD), caused by dystrophin deficiency, results in chronic inflammation and irreversible skeletal muscle degeneration. Moreover, the associated impairment of autophagy greatly contributes to the aggravation of muscle damage. We explored the possibility of using non-euphoric compounds present in Cannabis sativa, cannabidiol (CBD), cannabidivarin (CBDV) and tetrahydrocannabidivarin (THCV), to reduce inflammation, restore functional autophagy and positively enhance muscle function in vivo.
EXPERIMENTAL APPROACH
Using quantitative PCR, western blots and [Ca
KEY RESULTS
CBD and CBDV promoted the differentiation of murine C2C12 myoblast cells into myotubes by increasing [Ca
CONCLUSION AND IMPLICATIONS
We provide new insights into plant cannabinoid interactions with TRP channels in skeletal muscle, highlighting a potential opportunity for novel co-adjuvant therapies to prevent muscle degeneration in DMD patients.
LINKED ARTICLES
This article is part of a themed section on 8

Identifiants

pubmed: 30074247
doi: 10.1111/bph.14460
pmc: PMC6487563
doi:

Substances chimiques

Cannabinoids 0
Dystrophin 0
Endocannabinoids 0
Transient Receptor Potential Channels 0
Cannabidiol 19GBJ60SN5
tetrahydrocannabivarin 9 28172-17-0
Dronabinol 7J8897W37S
cannabidivarin I198VBV98I
Calcium SY7Q814VUP

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1568-1584

Informations de copyright

© 2018 The British Pharmacological Society.

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Auteurs

Fabio Arturo Iannotti (FA)

Endocannabinoid Research Group, Institute of Biomolecular Chemistry (ICB), National Research Council (CNR), Pozzuoli (NA), Italy.

Ester Pagano (E)

Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Naples, Italy.

Aniello Schiano Moriello (AS)

Endocannabinoid Research Group, Institute of Biomolecular Chemistry (ICB), National Research Council (CNR), Pozzuoli (NA), Italy.

Filomena Grazia Alvino (FG)

Telethon Institute of Genetics and Medicine (TIGEM), Pozzuoli, Italy.

Nicolina Cristina Sorrentino (NC)

Telethon Institute of Genetics and Medicine (TIGEM), Pozzuoli, Italy.

Luca D'Orsi (L)

Telethon Institute of Genetics and Medicine (TIGEM), Pozzuoli, Italy.

Elisabetta Gazzerro (E)

Gaslini Children's Hospital, Genoa, Italy.

Raffaele Capasso (R)

Department of Agricultural Sciences, University of Naples Federico II, Naples, Italy.

Elvira De Leonibus (E)

Telethon Institute of Genetics and Medicine (TIGEM), Pozzuoli, Italy.
Institute of Genetics and Biophysics (IGB), National Research Council, Naples, Italy.

Luciano De Petrocellis (L)

Endocannabinoid Research Group, Institute of Biomolecular Chemistry (ICB), National Research Council (CNR), Pozzuoli (NA), Italy.

Vincenzo Di Marzo (V)

Endocannabinoid Research Group, Institute of Biomolecular Chemistry (ICB), National Research Council (CNR), Pozzuoli (NA), Italy.

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