Distinct branches of the N-end rule pathway modulate the plant immune response.
Arabidopsis
/ genetics
Arabidopsis Proteins
/ genetics
Ascomycota
/ physiology
Ethylenes
/ metabolism
Hordeum
/ genetics
Oxidation-Reduction
Plant Diseases
/ immunology
Plant Growth Regulators
/ metabolism
Plant Immunity
Plant Stomata
/ genetics
Proteolysis
Pseudomonas syringae
/ physiology
Ubiquitin-Protein Ligases
/ genetics
Group VII Ethylene Response Factor transcription factor
N-end rule pathway
amino-terminal glutamine amidase
plant immunity
proteostasis
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
18
05
2018
accepted:
11
07
2018
pubmed:
18
8
2018
medline:
19
2
2020
entrez:
18
8
2018
Statut:
ppublish
Résumé
The N-end rule pathway is a highly conserved constituent of the ubiquitin proteasome system, yet little is known about its biological roles. Here we explored the role of the N-end rule pathway in the plant immune response. We investigated the genetic influences of components of the pathway and known protein substrates on physiological, biochemical and metabolic responses to pathogen infection. We show that the glutamine (Gln) deamidation and cysteine (Cys) oxidation branches are both components of the plant immune system, through the E3 ligase PROTEOLYSIS (PRT)6. In Arabidopsis thaliana Gln-specific amino-terminal (Nt)-amidase (NTAQ1) controls the expression of specific defence-response genes, activates the synthesis pathway for the phytoalexin camalexin and influences basal resistance to the hemibiotroph pathogen Pseudomonas syringae pv tomato (Pst). The Nt-Cys ETHYLENE RESPONSE FACTOR VII transcription factor substrates enhance pathogen-induced stomatal closure. Transgenic barley with reduced HvPRT6 expression showed enhanced resistance to Ps. japonica and Blumeria graminis f. sp. hordei, indicating a conserved role of the pathway. We propose that that separate branches of the N-end rule pathway act as distinct components of the plant immune response in flowering plants.
Substances chimiques
Arabidopsis Proteins
0
Ethylenes
0
Plant Growth Regulators
0
ethylene
91GW059KN7
PRT6 protein, Arabidopsis
EC 2.3.2.27
Ubiquitin-Protein Ligases
EC 2.3.2.27
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
988-1000Subventions
Organisme : Deutsche Forschungsgemeinschaft
Pays : International
Organisme : Pla de formació Universitat Jaume I
ID : PI-1B2015-33
Pays : International
Organisme : Landesgraduiertenförderung Sachsen-Anhalt
Pays : International
Organisme : Austrian Science Fund
ID : I1464-B16
Pays : International
Organisme : ScienceCampus Halle - Plant-based Bioeconomy
Pays : International
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/K000063/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/K000144/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M029441/1
Pays : United Kingdom
Organisme : Spanish Ministry of Economy and Competitiveness
ID : BIO2015-68130
Pays : International
Informations de copyright
© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust.