Nuclear export of mRNA molecules studied by SPEED microscopy.
Active Transport, Cell Nucleus
Algorithms
Animals
Eukaryota
/ metabolism
Humans
Image Interpretation, Computer-Assisted
/ methods
Imaging, Three-Dimensional
/ methods
Kinetics
Microscopy, Fluorescence
/ methods
Nuclear Pore
/ metabolism
RNA, Messenger
/ metabolism
Ribonucleoproteins
/ metabolism
Single Molecule Imaging
/ methods
3D live-cell imaging microscopy
Nucleocytoplasmic transport
Single-particle tracking
Super-resolution microscopy
Journal
Methods (San Diego, Calif.)
ISSN: 1095-9130
Titre abrégé: Methods
Pays: United States
ID NLM: 9426302
Informations de publication
Date de publication:
15 01 2019
15 01 2019
Historique:
received:
31
05
2018
revised:
19
07
2018
accepted:
10
08
2018
pubmed:
21
8
2018
medline:
12
9
2019
entrez:
21
8
2018
Statut:
ppublish
Résumé
The nuclear exit of messenger RNA (mRNA) molecules through the nuclear pore complex (NPC) is an essential step in the translation process of all proteins. The current limitations of conventional fluorescence and electron microscopy have prevented elucidation of how mRNA exports through the NPCs of live cells. In the recent years, various single-molecule fluorescence (SMF) microscopy techniques have been developed to improve the temporal and spatial resolutions of live-cell imaging allowing a more comprehensive understanding of the dynamics of mRNA export through native NPCs. In this review, we firstly evaluate the necessity of single-molecule live-cell microscopy in the study of mRNA nuclear export. Then, we highlight the application of single-point edge-excitation sub-diffraction (SPEED) microscopy that combines high-speed SMF microscopy and a 2D-to-3D transformation algorithm in the studies of nuclear transport kinetics and route for mRNAs. Finally, we summarize the new features of mRNA nuclear export found with SPEED microscopy as well as the reliability and accuracy of SPEED microscopy in mapping the 3D spatial locations of transport routes adopted by proteins and mRNAs through the NPCs.
Identifiants
pubmed: 30125665
pii: S1046-2023(18)30093-8
doi: 10.1016/j.ymeth.2018.08.005
pmc: PMC7138453
mid: NIHMS1574309
pii:
doi:
Substances chimiques
RNA, Messenger
0
Ribonucleoproteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
46-62Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM097037
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM116204
Pays : United States
Organisme : NIGMS NIH HHS
ID : R15 GM094041
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM122552
Pays : United States
Informations de copyright
Copyright © 2018 Temple University. Published by Elsevier Inc. All rights reserved.
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